Influence of supplement administration of omega‐3 on the subcutaneous tissue response of endodontic sealers in Wistar rats

Oliveira, Pedro Henrique Chaves de; Gomes‐Filho, João Eduardo; Rodrigues, Michael Júnio da Silva; Silva, Cristiane Cantiga da; Cardoso, C B M; Cosme‐Silva, Leopoldo; Ervolino, Edílson; Cintra, Luciano Tavares Ângelo

doi:10.1111/iej.13795

Cited by 5 publications

(4 citation statements)

References 41 publications

(101 reference statements)

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“…The number of animals was established following the protocols of previous studies, using an alpha error of 0.05% and 95% power to recognize a signi cant difference of 1 in the median scores, a minimum of seven animals per group was considered necessary. Considering that complications could occur during the study, one more animal was added for each period of analysis (7 and 30 days) [20,23].…”

Section: Subcutaneous Implantsmentioning

confidence: 99%

Cytotoxicity, biocompatibility and calcium deposition capacity of 45S5 bioglass experimental paste and Bio-C Temp: in vitro and in vivo study using Wistar rats

Benetti

Oliveira

Andrade

et al. 2023

Preprint

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Objectives To evaluate cytotoxicity, biocompatibility, calcium deposition, and collagen maturation of 45S5 bioglass experimental paste and Bio-C Temp compared to calcium hydroxide (Ca(OH)2) paste. Materials and methods 45S5 bioglass and Ca(OH)2 powder were mixed with distilled water (ratio 2:1) and Bio-C Temp is ready-for-use. Dental pulp cells were exposed to material extracts (½ and ¼ dilutions; 24, 48, 72h) for MTT and live/dead analysis. Polyethylene tubes filled with 45S5 bioglass, Bio-C Temp, and Ca(OH)2 pastes, or empty for control, were implanted on the dorsum of 16 rats. After 7 and 30 days (n=8/period), the rats were euthanised and the specimens processed for haematoxylin-eosin staining, von Kossa (vK) technique, and picrosirius red (PSR), or without staining for polarized light birefringence analysis (PL). Statistical analysis was applied (p<0.05). Results In vitro, there was no difference in cell viability among the Ca(OH)2, 45S5 bioglass and control, in all periods and extracts dilutions (p>0.05). Bio-C Temp was more cytotoxic than control in all periods and dilutions (p<0.05). In addition, it was more cytotoxic than 45S5 bioglass in 24 hours in 1:2 and 1:4 dilutions, and in 48 hours in the 1:2 dilution (p<0.05). In vivo, there was no difference between groups for any of the periods (p>0.05). However, there was a reduction in inflammation from 7 to 30 days for all groups. The fibrous capsule was thick for all groups at 7 days, and thin at 30 days. All materials showed positive structures for vK and birefringent in PL, which did not occur with the control. At 7 days, there were more immature fibers in control and 45S5 bioglass compared to other materials (p<0.05); at 30 days, 45S5 bioglass had more immature than mature fibers, being different from the other groups (p<0.05). Conclusions Bio-C Temp presented cytotoxicity superior to the experimental 45S5 bioglass and Ca(OH)2 pastes. On the other hand, the three pastes showed biocompatibility and ability to induce calcium deposition. In addition, the experimental bioglass paste allowed a marked and continuous proliferation of collagen fibers. Clinical relevance Bioglass-based intracanal medications are potential substitutes for traditional intracanal medications

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Section: Subcutaneous Implantsmentioning

confidence: 99%

Cytotoxicity, biocompatibility and calcium deposition capacity of 45S5 bioglass experimental paste and Bio-C Temp: in vitro and in vivo study using Wistar rats

Benetti

Oliveira

Andrade

et al. 2023

Preprint

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“…Considering three observation times, 48 animals were necessary in this study [4,27,29], They were caged in a controlled temperature and light cycle environment (22 ± 1 ° C, 70% humidity at 12 hours light and 12 hours dark), received solid diet and ad libitum water, except for the rst 24 hours after the intervention. The experimental procedures were approved and performed according to the guidelines of the ethical committee (process no.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…The implanted tubes were removed with the surrounding tissue and xed in 1% formalin solution at a pH of 7.0. The specimens were imbibed in para n and sectioned into 5-μm cuts for staining with hematoxylin-eosin, picrosirius red (PSR), immunohistochemistry for IL-6 and TNF-α and 10-μm cuts for staining with von Kossa and not stained for polarized light technique [27,34].…”

Section: Subcutaneous Implantmentioning

confidence: 99%

“…Considering that endodontic sealers can develop local in ammatory reactions on connective tissues and that melatonin is a potential regulator of the in ammatory process, the present study evaluated the in uence of melatonin supplementation on tissue's response to endodontic sealers by a consolidated method of exploring the biomaterials response, the implantation of polyethylene tubes in subcutaneous tissues of Wistar rats [27]. Null hypothesis: Melatonin would not interfere with the tissue's response to endodontic sealers.…”

Section: Introductionmentioning

confidence: 99%

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Influence of melatonin supplementation on tissue response of endodontic sealers in Wistar rats

de Oliveira,

Rodrigues,

Silva

et al. 2023

Preprint

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Objectives: Evaluate the influence of melatonin supplementation on tissue’s response to endodontic sealers in Wistar rats. Material and methods: Forty-eight Wistar rats had four polyethylene tubes implanted in their subcutaneous tissue, one empty (control) and three filled with endodontic sealers (AH Plus, Endofill and Sealapex). Half of the animals were supplemented with melatonin (TM) and the remaining with water (TW) for 15 days before the implantation until euthanasia. After 5, 15 and 30 days (n=8/group/period) tubes were removed and analyzed in H&E, immunohistochemistry, PSR, VK and POL. The results were statistically analyzed (p<0.05). Results: In TW, Endofill evoked a more intense inflammatory infiltrate compared to AH Plus and control in 30-day (p<0.05). In TM tubes, there was any difference in both periods (p>0.05), differences for Endofill (p=0.05) and Sealapex (p<0.05) was observed in TM animals in 30-day, presenting lower inflammatory infiltrate. For IL-6 and TNF-α it was observed that the immunolabeling was lower in TM animals in all periods, despite of the sealer. In addition, there was a higher percentage of mature fibers in TM groups at 5 days (p<0.05). Structures positive for von Kossa staining and birefringent to polarized light were observed only for Sealapex in all periods, regardless of melatonin supplementation. Conclusion: Melatonin influences inflammatory response of endodontic sealers, by modulating the inflammatory process and pre-inflammatory mediators, and stimulating tissue repair, without interfering on calcium deposition. Clinical Relevance: Evidence that melatonin can regulate inflammatory responses after the contact of tissues with biomaterials.

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Pathogenesis‐Guided Engineering: pH‐Responsive Imprinted Polymer Co‐Delivering Folate for Inflammation‐Resolving as Immunotherapy in Implant‐Related Infections

Costa,

Nagay,

Villa

et al. 2024

Adv Funct Materials

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Folate (FT) is a suitable targeting ligand for folate receptors (FOLR) overexpressed on inflamed cells. Thus, FT‐loaded polymers can be used as FOLRs‐targeted immunotherapy to positively modulate the inflammatory process. A novel biodegradable imprinted polymer with a FT delivery mechanism driven by pH changes [PCL‐MIP@FT] is designed with molecularly imprinted technology. The pH mechanism is validated in vitro, demonstrating that an acidic environment accelerated and increased the release of FT for a period of 7 days (∼100 µg mL−1). For the first time, FT receptors (FOLR‐1 and FOLR‐3) are discovered and also overexpressed on activated human gingival fibroblasts, representing a favorable target in the oral environment. Although FT itself does not have antimicrobial effects, the nanomechanical properties of biofilm are changed after topical FT administration. In vivo systemic toxicity of PCL‐MIP@FT has been demonstrated to be a safe biomaterial (up to 1.3 mg kg−1). When the PCL‐MIP@FT is assessed in the subcutaneous tissue, it promoted an alleviating inflammation and may be able to stimulate tissue repair. The present findings have demonstrated the reliable in vitro and in vivo anti‐inflammatory actions of FT‐loaded polymer and support its use as a novel drug‐free therapeutic platform for modulating and mitigating inflammatory responses in dental implant‐related infections.

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Influence of supplement administration of omega‐3 on the subcutaneous tissue response of endodontic sealers in Wistar rats

Cited by 5 publications

References 41 publications

Cytotoxicity, biocompatibility and calcium deposition capacity of 45S5 bioglass experimental paste and Bio-C Temp: in vitro and in vivo study using Wistar rats

Cytotoxicity, biocompatibility and calcium deposition capacity of 45S5 bioglass experimental paste and Bio-C Temp: in vitro and in vivo study using Wistar rats

Influence of melatonin supplementation on tissue response of endodontic sealers in Wistar rats

Pathogenesis‐Guided Engineering: pH‐Responsive Imprinted Polymer Co‐Delivering Folate for Inflammation‐Resolving as Immunotherapy in Implant‐Related Infections

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