Background
Autism spectrum disorder (ASD) is a developmental disorder characterized by social behavior deficits and stereotyped behaviors in childhood that lacks satisfactory medical intervention. Early swimming intervention is a noninvasive method combining enriched environment and exercise, which has been proven to improve brain development in young children and to treat neurodevelopmental diseases.
Methods
In this study, we tested the autism-like behavior of rats with deletions in exons 11–21 of the
Shank3
gene and evaluated the effect of early swimming intervention (from postnatal day 8 to 60) on the behavior of this animal model of autism. In addition, the transcriptomes of the striatal tissues of wild-type,
Shank3
knockout and
Shank3
knockout swimming groups rats were analyzed.
Results
Shank3
knockout rats exhibit core symptoms of autism, and early swimming improved the social and stereotyped behaviors in this autism rat model. Transcriptomics results revealed that compared to the wild-type group, 291 differentially expressed genes (DEGs) were identified in the striatum of the
Shank3
knockout group. Compared to
Shank3
knockout group, 534 DEGs were identified in the striatum of
Shank3
knockout swimming group. The DEGs annotated by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway shows that the impacts of
Shank3
deletion were primarily reflected in synaptic structure, development, morphology, receptor function and signaling, and swimming primarily changed the terms related to the synapses in the striatum of
Shank3
knockout rats, including the morphology, structure, composition, development and regulation of synapses.
Conclusion
Early swimming intervention can ameliorate behavioral abnormalities caused by
Shank3
knockout, by a mechanism that may involve the process of striatal synaptic development and should be further investigated.