Influence of Synthesis Parameters on the Particle Sizes of Nanostructured Calcium-Hydroxyapatite

Janaćković, Dj.; Petrovic-Prelevic, Irena; Kostic-Gvozdenovic, Lj.; Petrović, Rada; Jokanović, Vukoman; Uskoković, Dragan

doi:10.4028/www.scientific.net/kem.192-195.203

Cited by 13 publications

(6 citation statements)

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“…As a result, much effort has been focused on developing effective matrices to support repair by stimulating osteoblast differentiation and mineralization, as well as drug treatments to inhibit bone resorption. Many successful regenerative matrices used as implants, cements and coatings contain calcium phosphate (CaP) with submicron or nanoscale topographies 4–22. The presence of these particulates seems to improve protein adsorption,23, 24 osteoblast adhesion,25, 26 the regeneration of bone,27–31 and improves the integration and mechanical stability of titanium implants 32–36.…”

Section: Introductionmentioning

confidence: 99%

Uptake of calcium phosphate nanoshells by osteoblasts and their effect on growth and differentiation

Schmidt

Moran

Kent

et al. 2008

J Biomedical Materials Res

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The influence of calcium phosphate nanoshell materials on the uptake, viability, and mineralization of human fetal osteoblast cultures was evaluated. Proliferation rates and alkaline phosphatase activity of the cultures were unaffected by the addition of nanoshells to the growth media, but mineralization levels were enhanced by nearly 40%, in contrast to media prepared without nanoshells, or with other calcium phosphate nanomaterials. Nanoshells were internalized by macropinocytosis, and migrated toward the cell nucleus at a rate of 0.34 microm hr(-1). Dye-loaded nanoshells maintained high light emission intensity for over five days while inside the cells, where they could be used as intracellular markers for in vitro microscopic imaging. From these results, it appears that the CaP nanoshells could be developed into a safe sensor and delivery vehicle for osteoblast cell culture studies, whereas the carrier itself has intrinsic bioactivity and may itself upregulate the formation of new bone.

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Section: Introductionmentioning

confidence: 99%

Uptake of calcium phosphate nanoshells by osteoblasts and their effect on growth and differentiation

Schmidt

Moran

Kent

et al. 2008

J Biomedical Materials Res

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“…Nanočestični prah HA sintetisan je hidrotermalnom metodom prema već opisanom postupku [14][15][16]. Molski odnos Ca/P u prekusorskom rastvoru bio je 1.67, što odgovara stehiometrijskom odnosu kalcijuma i fosfora u hidroksiapatitu.…”

Section: Sinteza Kompozitnih Hidrogelovaunclassified

“…Poznato je da pore >100 µm omogućavaju osteogenezu, dok se za dobru vaskularizaciju preporučuju pore veće od 300 µm [26,27]. [14][15][16]. Važno je spomenuti da se ne razlikuju, ni po formi ni po veličini, od čestica HA korišćenih u svojstvu punioca u dentalnim kompozitima Ležaje i saradnika [24], što ukazuje na mogućnost poboljšanja mehaničkih svojstava kompozita prema mehanizmu već opisanom u pomenutom istraživanju.…”

Section: Morfologija/mikrostruktura čEstica/kompozitnih Hidrogelovaunclassified

Synthesis and properties of nano-hydroxyapatite/poly (methacrylic acid) composite hydrogels

Ugrinović¹,

Panic²,

Veljović³

et al. 2018

Tehnika

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Ciljevi-Cilj ove studije bio je razvoj biokompatibilnih kompozitnih hidrogelova, koji su slične strukture kao prirodno koštano tkivo, inkorporacijom ∼60 mas % kalcijum-hidroksiapatita (HA) u matricu hidrogela. Takođe, ispitan je uticaj neutralizacije prekursora polimerne mreže na kinetiku bubrenja i ravnotežni stepen bubrenja (SDeq). Metode-Kompozitni hidrogelovi, sastavljeni od HA i poli(metakrilne kiseline) (PMAA), sintetisani su slobodno-radikalskom polimerizacijom, pri različitim stepenima neutralizacije prekursora (SN). Svi sintetisani kompoziti sadrže visok maseni udeo HA (~60 mas %). Kompoziti su okarakterisani metodama skenirajuće elektronske mikroskopije (SEM) i dinamičko-mehaničke analize (DMA). SDeq i kinetika bubrenja ispitivani su u destilovanoj vodi i simuliranoj telesnoj tečnosti (SBF). Rezultati-Morfološka ispitivanja pokazala su uniformnu raspodelu i dobru povezanost sferičnih čestica apatita sa polimernom matricom. Analiza procesa bubrenja pokazala je proporcionalnu zavisnost SN i SDeq kompozita. Reološka ispitivanja ukazala su na obrnuto proporcionalnu zavisnost između SN i vrednosti modula sačuvane energije, koji su iznosili 55130 Pa u slučaju potpune neutralizacije i 77480 Pa u slučaju 0% neutralizacije. Značaj-Predstavljena je jednostavna metoda za sintezu kompozitnih hidrogelova sa visokim masenim udelom nanočestica punioca. Inkorporacijom nanočestica HA postignuto je značajno poboljšanje mehaničkih svojstava hidrogelova, dok je istovremeno pokazano da je promenom SN moguće kontrolisati kinetiku bubrenja hidrogelova.

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“…Sol-gel, spraydrying, hydrothermal synthesis, recently reviewed [31], have been developed to obtain nanoparticles with high specific surface area, high bioreabsorbability and containing foreign ions, mimicking bone hydroxyapatite chemical-physical and physiological behavior [32][33][34][35]. Mg 2þ ions which cause acceleration of nucleation kinetics of hydroxyapatite and inhibit its crystalline growth [36], are present in high concentration in bone tissue during the initial phases of osteogenesis and tend to disappear when the bone is mature [37].…”

Section: Biomimetic Hydroxyapatite: Porous and Substituted Apatitesmentioning

confidence: 99%

Hydroxyapatite Nanocrystals as Bone Tissue Substitute

Roveri

Palazzo

2003

Nanotechnologies for the Life Sciences

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OverviewNanoscience, where the properties of materials are exploited to innovative amazing applications, is involved in the size-dependent chemical and biological activity of bone substitute materials, which is indeed a fascinating field. The present chapter reviews the synthesis and chemical-physical characteristics of hydroxyapatite (HA) nano-crystals, which have excellent properties to represent an elective material covering a wide range of applications for bone substitution. We start from an examination of biogenic bone and tooth hydroxyapatite nanocrystals morphological and chemical-physical characteristics. The highlighted concepts have been used to review up-to date main new ideas on the preparation of synthetic apatitic bone substitutes mimicking the above biogenic properties, among which the nanosize is the basis of their self-assembly, self-mineralization and bone regeneration ability.Hydroxyapatite (HA) nanocrystals with high bioreabsorbability containing foreign ions and mimicking bone HA chemical-physical and physiological behaviour have been described. We have pointed to their possible use in preparing scaffolds with a porosity simulating that of spongy bone and upon which cells can be seeded, thereby developing ''in vitro autologous bone''. Biologically inspired HA nanocrystals/collagen composites have been reviewed, focusing on the role of a self-assembling strategy in conditioning the bone repairing activity of this biomaterial. Furthermore, considering that calcium phosphate/collagen composites are not limited to loading application, the possibility of preparing bio-inspired coating on the surface of metallic implants could be an advantageous approach.Finally, surface functionalization of HA nano-crystals with bioactive molecules makes them able to transfer information and to act selectively on the biological environment and can be considered one of the main future challenges for innovative bone substitute materials. 283 7.2

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Influence of Synthesis Parameters on the Particle Sizes of Nanostructured Calcium-Hydroxyapatite

Cited by 13 publications

References 0 publications

Uptake of calcium phosphate nanoshells by osteoblasts and their effect on growth and differentiation

Uptake of calcium phosphate nanoshells by osteoblasts and their effect on growth and differentiation

Synthesis and properties of nano-hydroxyapatite/poly (methacrylic acid) composite hydrogels

Hydroxyapatite Nanocrystals as Bone Tissue Substitute

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