Influence of Technological Treatments on the Functionality of <i>Bifidobacterium lactis</i> INL1, a Breast Milk‐Derived Probiotic

Zacarías, María Florencia; Souza, Tassia Costa; Zaburlín, Natalia; Cara, Denise Carmona; Reinheimer, Jorge Alberto; Nicoli, Jacques R.; Vinderola, Gabriel

doi:10.1111/1750-3841.13852

Cited by 16 publications

(9 citation statements)

References 45 publications

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“…This fact, attributed to different production processes and a long-term series of reinoculations, has also been described for L. acidophilus to such an extent that the outcome of the human intervention studies may be different [22]. Recently, the influence of technological treatments on the functionality of Bifidobacterium lactis INL1 has been confirmed [5] and the antifungal activity of Lactobacillus pentosus ŁOCK 0979 has been shown to be dependent from the presence of polyols and galactosyl-polyols in the culture medium [23]. Therefore, it is evident that genomic characterization of probiotic strains is not an appropriate solution to establish the consistency of a probiotic production.…”

Section: Discussionmentioning

confidence: 86%

The Epithelial Barrier Model Shows That the Properties of VSL#3 Depend from Where it is Manufactured

Palumbo

Lombardi

Cifone

et al. 2019

EMIDDT

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Background: VSL#3 has been extensively investigated and is currently recommended for the prevention and treatment of chronic pouchitis and ulcerative colitis. Nonetheless, in vitro and in vivo studies have recently shown variability in the VSL#3 efficacy often attributed to the manufacturing process. Objective: The aim was to comparatively study the in vitro effects of two VSL#3 preparations produced in different sites (named US- and Italy-made VSL#3) on CaCo-2 epithelial barrier model in terms of trans-epithelial electrical resistance (TEER), dextran flux and expression of Tight Junctions (TJ) proteins i.e. zonulin-1 (ZO-1) and occludin, in the absence or presence of a heat stress-related damage of monolayer. Methods: TEER was evaluated on CaCo-2 differentiated monolayers. Epithelial permeability of polarized monolayers was assessed by measuring the FITC-labeled dextran flux from the apical to basolateral chambers. ZO-1/occludin levels were analyzed by western blot analysis. A set of experiments was performed to compare the effects of both VSL#3 on TEER values, dextran flux and ZO-1/occludin expression in CaCo-2 monolayers after heat stress exposure. Results: US- and Italy-made VSL#3 have opposing effects on TEER values, dextran flux, and ZO- 1/occludin expression, being all these parameters negatively influenced just by Italy-made product. US-made probiotic did not affect baseline TEER, dextran flux and ZO-1 expression and strongly increased occludin levels. Of note, pre-treatment of monolayer with US-made VSL#3, but not Italy-made product, totally prevented the heat-induced epithelial barrier integrity loss. Conclusion: Our data trigger the need for reassessing efficacy or safety of the Italy-made VSL#3 considering intestinal epithelial barrier plays an important role in maintaining host health.

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Section: Discussionmentioning

confidence: 86%

The Epithelial Barrier Model Shows That the Properties of VSL#3 Depend from Where it is Manufactured

Palumbo

Lombardi

Cifone

et al. 2019

EMIDDT

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“…Finally, since the properties of the employed strain determine the efficacy of the treatment in each pathology, maintenance of the activity, viability, and growth efficacy of the probiotic upon technologic treatment should be verified. Indeed, the emerging evidence that manufacturing procedures may affect the efficacy and safety of probiotics imposes a special focus on choosing the probiotic to be administered [38,39,47,49,[60][61][62][63][64].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Low-Nickel Diet Combined with Oral Administration of Selected Probiotics on Patients with Systemic Nickel Allergy Syndrome (SNAS) and Gut Dysbiosis

Lombardi

Fiasca

Minelli³

et al. 2020

Nutrients

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Background: Nickel (Ni) oral consumption may elicit systemic reactions in patients affected by systemic nickel allergy syndrome (SNAS), including gastrointestinal symptoms, which in turn are associated with gut dysbiosis. We evaluated the effects of a low-Ni diet alone or in combination with the oral consumption of appropriate probiotics on Ni-sensitivity and urinary dysbiosis markers in SNAS patients. Methods: n = 51 patients with SNAS and concomitant intestinal dysbiosis were enrolled in the study. According to the urinary indican/skatole levels, quantified through a colorimetric and a high-performance liquid chromatographic method, respectively, patients were assigned to a dysbiosis type/grade and followed a low-Ni diet for three months. Along with the diet, 22 patients also consumed probiotics based on the dysbiosis type. In particular, a Lactobacilli- or Bifidobacteria-containing formulation was administered to patients with fermentative or putrefactive dysbiosis, respectively, while a broad-spectrum probiotic formulation containing both Lactobacilli and Bifidobacteria was administered to patients with mixed dysbiosis. After three months, patients were invited to repeat the Ni-stimulation and the dysbiosis tests. Results: The fermentative dysbiosis group represented the largest group followed by the mixed dysbiosis group, while only two patients had putrefactive dysbiosis. Overall, at three months of treatment in general (diet alone with or without probiotics), the Ni-sensitivity and dysbiosis levels were strongly ameliorated. The association of a low-Ni diet with a specific probiotic oral supplementation was significantly more effective in decreasing dysbiosis levels or reaching eubiosis than with diet alone. Conclusion: Our results, while confirming the benefits of a low-Ni diet in SNAS patients, strongly support that appropriate adjuvant treatment with probiotics significantly helps to improve intestinal dysbiosis or restore a healthy microbiota.

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“…Finally, as strongly suggested by an increasing number of studies [39][40][41][42][43][44][45], a careful selection of the probiotic agent, standardization of the dose and detailed characterization of the beneficial effects are essential when considering use of a probiotic for the management of NSAID-induced enteropathy. Indeed, the bioavailability of nutrients, drugs, probiotics or other substances administered orally depends on intestinal barrier integrity, which can be compromised in various inflammatory gut diseases, including NSAID-induced enteropathy.…”

Section: Discussionmentioning

confidence: 99%

Influence of Microbiota on NSAID Enteropathy: A Systematic Review of Current Knowledge and the Role of Probiotics

et al. 2020

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Microbiota are increasingly studied, providing more precise information on their important role in physiologic processes. They also influence some pathologic processes, such as NSAIDinduced enteropathy. This side effect is much more diffuse than it has been described in the past. It derives mainly from the local action of the medicines and is caused by the local binding of gram-negative bacterial lipopolysaccharides and infiltration of neutrophils into the intestinal mucosa. The initial interest in the interaction between these damages and microbiota is very old, but new and interesting data are available. This review aims to focus on recent studies on NSAID-induced enteropathy, an often-underestimated medical condition, and on the influence of microbiota on this condition. Apart from the broadly investigated use of antibiotics and other mucosal protective solutions, this systematic review focuses mostly on the use of probiotics, which directly influence intestinal microflora. Other important factors influencing NSAID-induced enteropathy, such as sex, advanced age, infection and use of proton pump inhibitors, are also discussed.

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Influence of Technological Treatments on the Functionality of Bifidobacterium lactis INL1, a Breast Milk‐Derived Probiotic

Abstract: This work provides evidence about the influence of technology on the functionality of probiotics, which may help probiotics and functional food manufacturers to take processing into consideration when assessing the functionality of new strains.

Cited by 16 publications

References 45 publications

The Epithelial Barrier Model Shows That the Properties of VSL#3 Depend from Where it is Manufactured

The Epithelial Barrier Model Shows That the Properties of VSL#3 Depend from Where it is Manufactured

The Effects of Low-Nickel Diet Combined with Oral Administration of Selected Probiotics on Patients with Systemic Nickel Allergy Syndrome (SNAS) and Gut Dysbiosis

Influence of Microbiota on NSAID Enteropathy: A Systematic Review of Current Knowledge and the Role of Probiotics

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