Influence of temperature and polyaromatic contaminants on CYP1A levels in North Sea dab (Limanda limanda)

Sleiderink, Hedwig M.; Beyer, Jonny; Scholtens, Enit; Goksøyr, Anders; Nieuwenhuize, J.; Liere, J.M. van; Everaarts, J.M.; Boon, Jan P.

doi:10.1016/0166-445x(94)00080-a

Cited by 67 publications

(18 citation statements)

References 31 publications

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“…On a wet weight basis, PCBA60 tissue concentrations ranged from 10 to about 2300 ng g -1 . Levels of PCB in dab were similar to those reported by Büther (1988) for this species in the southern North Sea, but up to two orders of magnitude higher than reported by Sleiderink and Boon (1995) and Sleiderink et al (1995b) for the same area. Lipidbased liver burden of PCBA60 in flounder at times was up to one order of magnitude higher than in dab (Fig.…”

Section: Resultssupporting

confidence: 73%

“…A strong influence of temperature on EROD activity has been shown to exist, although the temperature effect does not necessarily show a causal relationship but is rather coincidental with the state of maturity the fish have reached (see also Lange et al 1998). Krüner and Westernhagen (1995) showed experimentally that turbot (Scophthalmus maximus) displayed higher EROD activity with increasing temperatures, while Lange et al (1992) claimed higher EROD activity at lower temperatures in dab, and Sleiderink et al (1995b) even stated that in their investigations of EROD in North Sea dab the effect of water temperature was dominant over the effect of PCB contamination; EROD activity being higher at low temperatures. Thus, differences in water temperature between sampling stations may have obscured effects of PCB contamination on EROD activity or an existing reaction may even have been suppressed by chemicals such as tributyltin (TBT) (Fent and Stegeman 1993;Morcillo and Porte 1997) or tetrachlorobiphenyl (TCB), as found out by Monosson and Stegemann (1991) …”

Section: Resultsmentioning

confidence: 99%

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Ethoxyresorufin O-deethylase (EROD) activity in the liver of dab ( Limanda limanda L.) and flounder ( Platichthys flesus L.) from the German Bight. EROD expression and tissue contamination

Westernhagen

Krüner

Broeg

1999

Helgoland Marine Research

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Abstract. Ethoxyresorufin O-deethylase (EROD) activity was measured in the liver of dab (Limanda limanda) and flounder (Platichthys flesus) from the German Bight (southern North Sea) and compared with muscle and liver polychlorinated biphenyl (PCB) concentrations in an attempt to relate EROD activity to PCB body burden. In none of the different datasets (species-, tissue-or matrixdependent) was a significant (P<0.05) correlation between PCB tissue contamination and EROD activity found. Yet EROD activity was significantly correlated with polycyclic aromatic hydrocarbons (PAH) levels (phenanthrene, fluoranthene, pyrene) in muscle tissue, indicating a possible dependence of EROD expression on other ubiquitous organic contaminants, thus making it a suitable biomarker for general pollution.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Ethoxyresorufin O-deethylase (EROD) activity in the liver of dab ( Limanda limanda L.) and flounder ( Platichthys flesus L.) from the German Bight. EROD expression and tissue contamination

Westernhagen

Krüner

Broeg

1999

Helgoland Marine Research

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“…From the few studies that determined the gill filament EROD activities, the baseline activities in rainbow trout varied by 200-fold as it ranged from 0.001 to 0.2 pmol/filament tip/ min (Jö nsson et al 2002(Jö nsson et al , 2003. It is well known that differences in physiological factors and seasonal variations modulate hepatic EROD activities and expression of CYP1A isozyme (Stegeman and Hahn 1994;Sleiderink et al 1995). Variations in EROD basal activities between studies and fish species suggest the need for inclusion of control fish in each study as standardization of parameters such as source, species, age, weight, and sex of fish, season as well as sampling and laboratory procedures might not warranty the extrapolation of laboratory to field based results.…”

Section: Discussionmentioning

confidence: 98%

Influence of 17α-ethynylestradiol on CYP1A, GST and biliary FACs responses in male African sharptooth catfish (Clarias gariepinus) exposed to waterborne Benzo[a]Pyrene

et al. 2006

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Dose dependent effects of Benzo[a]pyrene (BaP) on cytochrome P4501A (CYP1A), glutathione-S-transferase (GST) and fluorescent aromatic compounds (FACs) metabolites biomarker responses were studied in African sharptooth catfish (Clarias gariepinus) following 24 h of waterborne exposures. Based on biomass of C. gariepinus in different tanks, BaP concentrations of 1.60, 3.44, and 18.21 microg/L that corresponded to 0.5, 1.0, and 5.0 mg/kg body weight were used. Significant induction of EROD activities in gill filaments was observed at all doses and the accumulation of FACs metabolites in bile was significantly different between groups. Accumulation of FACs metabolites in bile strongly correlated (r (2) = 0.99) with BaP doses. Hepatic EROD activities were undetectable and no effect on GST activities was observed. The highest dose of BaP from the dose dependent study was further studied to assess the interactive and temporal responses of C. gariepinus on CYP1A, GST, and FACs metabolites biomarkers following exposure to either BaP alone, 17alpha-ethynylestradiol (EE(2)) alone or a combination of both compounds at concentrations of 54.17 microg/L for BaP, 51.38 microg/L for EE(2) and 54.44 microg/L for each of both compounds. Based on biomass in each tank, these concentrations corresponded to 5 mg/kg body weight. While a group of six fish was sacrificed on day 0 from the control tank only, other groups of six fish were sacrificed after 1, 3, and 6 days of exposure from the control and exposed groups. Maximum induction of gill filament and hepatic EROD activities was observed after 1 day of exposure. Both EROD activities in gill filaments and liver were significantly induced by exposure to BaP alone or co-administration with EE(2). Gill filament EROD induction was significantly inhibited (50%) by co-administration of BaP and EE(2) compared to administration of BaP alone. Levels of FACs in bile for BaP and BaP + EE(2) exposed groups were significantly different from the control at all doses and time points. A significant induction of GST activities was observed in fish exposed to BaP and BaP + EE(2) after 3 days. Exposure to EE(2) alone caused significant induction of this enzyme after day 6. This study reports for the first time the significant antagonistic influence of EE(2) on BaP in gills of fish following waterborne exposures. The results also indicate that chemical mixtures may affect biomarker responses differently from compounds administered alone and that the sensitivity of CYP1A to interactive chemicals is different in gills and liver.

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“…While there is no evidence to suggest that environmental factors such as salinity and temperature significantly affect the formation of DNA adducts these factors should always be considered, as it is known that cellular detoxification systems (e.g. Cyp1A) are influenced by changes in environmental variables (Sleiderink et al, 1995).…”

Section: Ecological Relevance and Validation For Use In The Fieldmentioning

confidence: 99%

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

Brooks

Farmen

2013

Journal of Shellfish Research

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Influence of temperature and polyaromatic contaminants on CYP1A levels in North Sea dab (Limanda limanda)

Cited by 67 publications

References 31 publications

Ethoxyresorufin O-deethylase (EROD) activity in the liver of dab ( Limanda limanda L.) and flounder ( Platichthys flesus L.) from the German Bight. EROD expression and tissue contamination

Ethoxyresorufin O-deethylase (EROD) activity in the liver of dab ( Limanda limanda L.) and flounder ( Platichthys flesus L.) from the German Bight. EROD expression and tissue contamination

Influence of 17α-ethynylestradiol on CYP1A, GST and biliary FACs responses in male African sharptooth catfish (Clarias gariepinus) exposed to waterborne Benzo[a]Pyrene

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

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