Influence of temperature during and after sodium azide treatment on M1 damage and M2 chlorophyll mutation in barley (Hordeum vulgare L.)

Hasegawa, Hiroshi; Inoue, Masayoshi

doi:10.1016/0098-8472(84)90054-6

Cited by 5 publications

(1 citation statement)

References 8 publications

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“…Gichner and Veleminsky (1977) showed that concentrations of 1-5 mmol/L NaN 3 decreased the germination rate of Arabidopsis thaliana. Others, such as Hasegawa and Inoue (1984) and Nilan et al (1975), have reported that NaN 3 affects the germination rate of barley. This inhibitory effect of NaN 3 on seed germination was not seen at Day 14 of the germination period.…”

Section: Discussionmentioning

confidence: 95%

Effects of the Application of Different Concentrations of NaN3 for Different Times on the Morphological and Cytogenetic Characteristics of Barley (Hordeum vulgare L.) Seedlings

Ilbaş

Eroğlu

2005

J Integrative Plant Biology

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Sodium azide (NaN 3 ) has been used in many biological studies as a mutagen. In the present study, the morphological and cytogenetic effects of NaN 3 on barley (Hordeum vulgare L.) seedlings were investigated. Seeds of barley were treated with different concentrations of NaN 3 (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mmol/L) and applied for different periods of time (3 and 4 h). Parameters investigated, except for the mitotic index, were determined on Days 7 and 14. Increasing concentrations of NaN 3 affected germination rates on Days 7 and 14 following application for 3 and 4 h. Although the length of the roots and leaves was affected by treatment with NaN 3 on Day 14 of the germination period, coleoptile length was affected by NaN 3 treatment on Day 7. Increasing concentrations of NaN 3 and increased treatment period decreased the mitotic index compared with the untreated control. The inhibitory effects of NaN 3 on the mitotic index indicate that NaN 3 can have genotoxic and mutagenic effects on barley seedlings.

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Section: Discussionmentioning

confidence: 95%