Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity

Bonaventura, Giovanni Di; Piccolomini, R; Paludi, Domenico; D’Orio, Vincenzo; Vergara, Alberto; Conter, Mauro; Ianieri, Adriana

doi:10.1111/j.1365-2672.2007.03688.x

Cited by 382 publications

(276 citation statements)

References 44 publications

(91 reference statements)

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“…It is not surprising that the surface structures and, thereby, the surface hydrophobicity may vary between isolates of different serotypes. Variations due to adaptations to different temperatures could also be expected, as this has been reported in other species, e.g., Listeria monocytogenes (27) and Serratia marcescens (36).…”

Section: Discussionmentioning

confidence: 94%

“…Different biofilm-forming abilities between serotypes of Listeria monocytogenes (27,28) and between serovars of Salmonella (18,29) have also been reported. Little has previously been reported on possible differences in biofilm production between serotypes or serogroups of E. coli (30), although differences between strains have been published by several (31)(32)(33)(34).…”

Section: Discussionmentioning

confidence: 99%

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Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

Nesse

Sekse

Berg

et al. 2014

Appl Environ Microbiol

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The biofilm-producing abilities of potentially human-pathogenic serotypes of Escherichia coli from the ovine reservoir were studied at different temperatures and on different surfaces. A possible influence of the hydrophobicity of the bacterial cells, as well as the presence of two virulence factors, the Shiga toxin-encoding (Stx) bacteriophage and the eae gene, was also studied. A total of 99 E. coli isolates of serotypes O26:H11, O103:H2, and O103:H25 isolated from sheep feces were included. The results show that isolates of all three E. coli serotypes investigated can produce biofilm on stainless steel, glass, and polystyrene at 12, 20, and 37°C. There was a good general correlation between the results obtained on the different surfaces. E. coli O103:H2 isolates produced much more biofilm than those of the other two serotypes at all three temperatures. In addition, isolates of serotype O26:H11 produced more biofilm than those of O103:H25 at 37°C. The hydrophobicity of the isolates varied between serotypes and was also influenced by temperature. The results strongly indicated that hydrophobicity influenced the attachment of the bacteria rather than their ability to form biofilm once attached. Isolates with the eae gene produced less biofilm at 37°C than isolates without this gene. The presence of a Stx bacteriophage did not influence biofilm production. In conclusion, our results show that potentially human-pathogenic E. coli from the ovine reservoir can form biofilm on various surfaces and at several temperatures relevant for food production and handling.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

Nesse

Sekse

Berg

et al. 2014

Appl Environ Microbiol

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“…The ability of bacteria, including L. monocytogenes, to adhere to and colonize surfaces is influenced by many environmental factors (26) that affect the physiological characteristics of bacteria (6,11,33) and/or alter the chemistry of the surface (26). The type and the composition of food residue on foodprocessing equipment have been suggested to influence both the population levels of attached cells and their resistance to disinfectants (6,20,24,33,44).…”

Section: Discussionmentioning

confidence: 99%

Listeria monocytogenes Attachment to and Detachment from Stainless Steel Surfaces in a Simulated Dairy Processing Environment

Poimenidou¹,

Belessi²,

Giaouris

et al. 2009

Appl Environ Microbiol

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The presence of pathogens in dairy products is often associated with contamination via bacteria attached to food-processing equipment, especially from areas where cleaning/sanitation is difficult. In this study, the attachment of Listeria monocytogenes on stainless steel (SS), followed by detachment and growth in foods, was evaluated under conditions simulating a dairy processing environment. Initially, SS coupons were immersed in milk, vanilla custard, and yogurt inoculated with the pathogen (10 7 CFU/ml or CFU/g) and incubated at two temperatures (5 and 20°C) for 7 days. By the end of incubation, cells were mechanically detached from coupons and used to inoculate freshly pasteurized milk which was subsequently stored at 5°C for 20 days. The suspended cells in all three products in which SS coupons were immersed were also used to inoculate freshly pasteurized milk (5°C for 20 days). When SS coupons were immersed in milk, shorter lag phases were obtained for detached than for planktonically grown cells, regardless of the preincubation temperature (5 or 20°C). The opposite was observed when custard incubated at 20°C was used to prepare the two types of inocula. However, in this case, a significant increase in growth rate was also evident when the inoculum was derived from detached cells. In another parallel study, while L. monocytogenes was not detectable on SS coupons after 7 days of incubation (at 5°C) in inoculated yogurt, marked detachment and growth were observed when these coupons were subsequently transferred and incubated at 5°C in fresh milk or/and custard. Overall, the results obtained extend our knowledge on the risk related to contamination of dairy products with detached L. monocytogenes cells.

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“…Formation of L. monocytogenes biofilms can occur with various counts and under on various surfaces, such as on Buna-N rubber (23), at a level of 2.1 log CFU/ cm 2 on 0.8% minimal NaCl medium (24), and on glass and steel surfaces (25). The experimental conditions of this study favored formation of a biofilm on stainless steel after 48 h. Serotype 1/2a is commonly isolated from food so emphasis on this serotype can deepen our understanding of cross contamination of foods.…”

Section: Resultsmentioning

confidence: 99%

Transfer of Listeria monocytogenes between abiotic surfaces under different weights

Lee¹,

Chai²,

Mahyudin³

et al. 2014

Food Sci Biotechnol

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Cross contamination of Listeria monocytogenes from two different surfaces (plastic wrappers and stainless steel coupons) was simulated using a series of different weights. Enumeration of transfer was based on surface bacterial counts of biofilm stained surfaces. Direct contact between 2 surfaces has a constant rate of transfer that is independent of the pressure applied. This is the first report on the study of cross contamination between surfaces using pressure to illustrate transfer of bacteria in a food processing line.

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Influence of temperature on biofilm formation by Listeria monocytogenes on various food-contact surfaces: relationship with motility and cell surface hydrophobicity

Cited by 382 publications

References 44 publications

Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

Listeria monocytogenes Attachment to and Detachment from Stainless Steel Surfaces in a Simulated Dairy Processing Environment

Transfer of Listeria monocytogenes between abiotic surfaces under different weights

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