Influence of temperature on biological phosphorus removal: process and molecular ecological studies

Brdjanović, Damir; Logemann, Susanne; Loosdrecht, M.C.M. van; Hooijmans, Christine M.; Alaerts, Guy J.; Heijnen, Joseph J.

doi:10.1016/s0043-1354(97)00322-9

Cited by 106 publications

(62 citation statements)

References 20 publications

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“…Indeed, the observed acetate uptake rate was higher than has been observed for PAOs at 308C under short-term as well as long-term operation, 0.13-0.18 C-mol/C-mol h (Brdjanovic et al, 1997(Brdjanovic et al, , 1998Lopez-Vazquez et al, 2007), indicating a competitive advantage for GAOs at this temperature, which explains the absence of PAOs in the present study.…”

Section: Enrichment Of Gaoscontrasting

confidence: 52%

The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates

Bengtsson

2009

Biotech & Bioengineering

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Production of polyhydroxyalkanoates (PHAs) by an open mixed culture enriched in glycogen accumulating organisms (GAOs) under alternating anaerobic-aerobic conditions with acetate as carbon source was investigated. The culture exhibited a stable enrichment performance over the 450-day operating period with regards to phenotypic behavior and microbial community structure. Candidatus Competibacter phosphatis dominated the culture at between 54% and 70% of the bacterial biomass throughout the study, as determined by fluorescence in situ hybridization. In batch experiments under anaerobic conditions, PHA containing 3-hydroxybutyrate (3HB) and 27 mol-% 3-hydroxyvalerate (3HV) was accumulated up to 49% of cell dry weight utilizing the glycogen pool stored in the SBR cycle. Under aerobic and ammonia limited conditions, PHA comprising only 3HB was accumulated to 60% of cell dry weight. Glycogen was consumed during aerobic PHA accumulation as well as under anaerobic conditions, but with different stoichiometry. Under aerobic conditions 0.31 C-mol glycogen was consumed per consumed C-mol acetate compared to 0.99 under anaerobic conditions. Both the PHA biomass content and the specific PHA production rate obtained were similar to what is typically obtained using the more commonly applied aerobic dynamic feeding strategy.

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Section: Enrichment Of Gaoscontrasting

confidence: 52%

The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates

Bengtsson

2009

Biotech & Bioengineering

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“…This agrees with the study of Brdjanovic et al 17 which indicated that the stoichiometry of the PAOs' activities under anaerobic condition were insensitive to a short-term temperature change. Associated with the phosphorus release, the COD was taken up; nevertheless, in a decreasing manner as the temperature increased strikingly.…”

Section: Pulse Alterationsupporting

confidence: 93%

“…Therefore, there is a strong need to evaluate the impact of temperature variation on the EBPR process. Many studies on the effects of temperature on the efficiency of the EBPR process have been conducted; [11][12][13][14][15][16][17] however, the results are inconsistent, possibly due to differences in thermal-stress scenario. This study concentrates on the impact of temperature shock, which is likely to happen in field practice, on EBPR performance.…”

Section: Scienceasia Scienceasia Scienceasiamentioning

confidence: 99%

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Panswad¹,

Doungchai²,

Anotai³

2003

ScienceAsia

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An enhanced biological phosphorus removal (EBPR) process is currently one of the most economical and practical ways to reduce high phosphorus content in wastewaters. Under sequential conditions of anaerobic and aerobic environments, the phosphateaccumulating organisms (PAOs), which are able to accumulate intracellular phosphorus in a much higher quantity than other microorganisms, become predominant. In anaerobic stage, the PAOs absorb readily biodegradable substrate by destroying their storage polyphosphate to form adenosine triphosphate (ATP) and adenosine diphosphate (ADP), sequentially, and releasing free phosphate to the bulk solution. The substrate taken up is metabolized in the following aerobic stage to produce energy some of which replenishes the polyphosphate storage pool by absorbing phosphate from the bulk solution. The amount taken up aerobically is more than that released anaerobically; hence the phosphorus in the liquid phase is reduced. Although many researchers have recently attempted to investigate the ecology and microbiology of the EBPR processes using either culture-dependent or -independent approaches, the results are still inconclusive.1 In parallel to microbiologial approach, several investigators have tried to solve this problem through biochemical models which are developed based on mechanistic considerations of the mixed cultures in activated sludge under control conditions. These models, e.g., Comeau model, 2 Mino model, 3 metabolic model, [4][5][6] and enhanced culture kinetic model, 7-9 involve stoichiometry and process kinetic rate equations. Many EBPR systems have been designed and constructed by using the simplified versions of these models. Although most of them perform reliably as expected, some behave unpredictably. One possible explanation of most interest to the researchers is that, under certain operating conditions, other microorganisms are more selectively favored than the PAOs, hence diminishing the EBPR performance. The microbial group believed to be a major competitor of the PAOs are the glycogenaccumulating organisms (GAOs), which also assimilate substrates under anaerobic conditions. 10 As named, this microbial group aerobically synthesizes and stores glycogen as its primary energy source. Since polyphosphate is not involved in substrate assimilation, ABSTRACT: Four anaerobic-aerobic sequencing batch reactors with 9-day of sludge age were operated under a 12-hour cycle of 5, 290, 360, 60, and 5 minutes for the filling, anaerobic, aerobic, settling, and withdrawal periods, respectively. The results indicated that increasing temperature from the phosphate-accumulating organisms (PAOs) preferred temperature of 20°C by either pulse (5°C after every 5 days) or step (1°C per day) manner had negative impacts on the PAOs' activities, particularly on the aerobic phosphorus uptake. However, the pulse-increase scenario, especially from 30 to 35°C, caused more severe impacts than the stepincrease scenario. On the contrary, the shock in a decreasing manner regardless of experimen...

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“…It is still unknown whether the EBPR deterioration occurred because: (a) PAO are not able to adapt to higher temperature levels (>208C) giving GAO the chance to proliferate, (b) GAO have metabolic advantages over PAO at high temperatures, or (c) a combination of both previous causes. Brdjanovic et al (1997Brdjanovic et al ( , 1998a carried out a systematic study on an enriched PAO culture in order to understand the short-and long-term temperature effects on the EBPR process from 5 to 308C. According to their observations, the observed temperature dependencies at short-(a few hours) and long-term (weeks) were well described by applying the Arrhenius temperature coefficients calculated in that research.…”

Section: Introductionmentioning

confidence: 92%

Short‐term temperature effects on the anaerobic metabolism of glycogen accumulating organisms

López-Vázquez

Song

Hooijmans

et al. 2006

Biotech & Bioengineering

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Proliferation of glycogen accumulating organisms (GAO) has been identified as a potential cause of enhanced biological phosphorus removal (EBPR) failure in wastewater treatment plants (WWTP). GAO compete for substrate with polyphosphate accumulating organisms (PAO) that are the microorganisms responsible for the phosphorus removal process. In the present article, the effects of temperature on the anaerobic metabolism of GAO were studied in a broad temperature range (from 10 to 40 degrees C). Additionally, maximum acetate uptake rate of PAO, between 20 and 40 degrees C, was also evaluated. It was found that GAO had clear advantages over PAO for substrate uptake at temperatures higher than 20 degrees C. Below 20 degrees C, maximum acetate uptake rates of both microorganisms were similar. However, lower maintenance requirements at temperature lower than 30 degrees C give PAO metabolic advantages in the PAO-GAO competition. Consequently, PAO could be considered to be psychrophilic microorganisms while GAO appear to be mesophilic. These findings contribute to understand the observed stability of the EBPR process in WWTP operated under cold weather conditions. They may also explain the proliferation of GAO in WWTP and thus, EBPR instability, observed in hot climate regions or when treating warm industrial effluents. It is suggested to take into account the observed temperature dependencies of PAO and GAO in order to extend the applicability of current activated sludge models to a wider temperature range.

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Influence of temperature on biological phosphorus removal: process and molecular ecological studies

Cited by 106 publications

References 20 publications

The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates

The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates

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Short‐term temperature effects on the anaerobic metabolism of glycogen accumulating organisms

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