Influence of temperature on laser ablation fractionation during ICP-MS analysis for 213 nm and 266 nm laser wavelength micro-sampling

Jarošová, Monika; Walaszek, D.; Wagner, Barbara; Milde, David; Bulska, Ewa

doi:10.1039/c6ja00182c

Cited by 3 publications

(5 citation statements)

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“…In comparison with ablation at 193 and 213 nm, the 266 nm laser has a higher thermal effect . To observe the thermal effect of LA on fresh biological tissues with a high water content, fresh plant leaves were ablated at room temperature and under cryogenic conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The observations herein indicated that cryogenic conditions could substantially improve the precision and accuracy for LA-ICP-MS, which was consistent with previous reports. 22,26 The ratio of U/Th is a significant index to evaluate the fractionation effect in LA-ICP-MS. 27,28 Figure S5E shows the significant fractionation effect of the U/Th ratio by ablation of NIST 612 with a 266 nm wavelength laser source at room temperature (20 °C). On the other hand, however, virtually no fractionation effect was observed under cryogenic conditions (−26 °C) with a U/Th ratio close to 1.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In comparison with ablation at 193 and 213 nm, the 266 nm laser has a higher thermal effect. 26 To observe the thermal effect of LA on fresh biological tissues with a high water content, fresh plant leaves were ablated at room temperature and under cryogenic conditions. Five different spot sizes ranging from 30 to 70 μm were adopted to perform LA at a laser energy of 5 J/cm 2 and a scan speed of 100 μm/s.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…This makes cryogenic laser ablation (LA) an excellent biological tissue imaging technique, i.e., cryo-imaging. Cryogenic LA was applied in the measurement of uranium isotope ratios and metal imaging in biological samples. , The control of temperature in a cryogenic LA cell and the influence of temperature were also evaluated. , Nevertheless, cryogenic LA is still under investigation, and to date, the reported laboratory-built cryogenic LA cells exhibit certain limitations, e.g., long waiting times and a difficulty in replacing parts. A long waiting time may cause loss of water from fresh biological tissues and adversely affect the elemental imaging.…”

Section: Introductionmentioning

confidence: 99%

“…23,24 The control of temperature in a cryogenic LA cell and the influence of temperature were also evaluated. 25,26 Nevertheless, cryogenic LA is still under investigation, and to date, the reported laboratory-built cryogenic LA cells exhibit certain limitations, e.g., long waiting times and a difficulty in replacing parts. A long waiting time may cause loss of water from fresh biological tissues and adversely affect the elemental imaging.…”

Section: ■ Introductionmentioning

confidence: 99%

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Cryogenic Laser Ablation in a Rapid Cooling Chamber Ensures Excellent Elemental Imaging in Fresh Biological Tissues

et al. 2022

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Laser ablation inductively coupled plasma mass spectrometry imaging of biologically significant targets largely relies on maintaining the original structures of samples. The temperature regulation capability of the ablation cell is crucial. Herein, a rapid cooling cryogenic sample cell (RCCSC) was developed. In the RCCSC chamber, the temperature reduces to −20 °C in 4 min with a minimum 10 h variation of ±0.1 °C at −26 °C. Improvements on the precision were achieved for the elements of interest in NIST 612 and spiked agarose gel under cryogenic conditions. The limits of detection improved by up to 1.57, 1.70, 3.26, and 1.33 fold for 63Cu, 66Zn, 57Fe, and 140Ce in agarose gel, respectively, were obtained under cryogenic conditions compared with those at room temperature. In a time period of testing (10 h), the cryogenic ablation maintains the native state of biological tissues with a high water content to ensure better elemental imaging by reducing thermal effects in ablation and suppressing evaporation of water. The rapid cooling cryogenic ablation significantly improves elemental imaging, as demonstrated by the imaging of various elements in coriander leaves. The present study may provide further insights into elemental distributions in fresh biological samples.

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Section: Resultsmentioning

confidence: 99%