Influence of Temperature on the Bacterial Community in Substrate and Extracellular Enzyme Activity ofAuricularia cornea

Abstract

Temperature is an important environmental factor that can greatly influence the cultivation of Auricularia cornea. In this study, lignin peroxidase, laccase, manganese peroxidase, and cellulose in A. cornea fruiting bodies were tested under five different temperatures (20 °C, 25 °C, 30 °C, 35 °C, and 40 °C) in three different culture periods (10 days, 20 days and 30 days). In addition, the V4 region of bacterial 16S rRNA genes in the substrate of A. cornea cultivated for 30 days at different temperatures were sequenced using next-generation sequencing technology to explore the structure and diversity of bacterial communities in the substrate. Temperature and culture days had a significant effect on the activities of the four enzymes, and changes in activity were not synchronized with changes in temperature and culture days. Overall, we obtained 487,694 sequences from 15 samples and assigned them to 16 bacterial phyla. Bacterial community composition and structure in the substrate changed when the temperature was above 35 °C. The relative abundances of some bacteria were significantly affected by temperature. A total of 35 genera at five temperatures in the substrate were correlated, and 41 functional pathways were predicted in the study. Bacterial genes associated with the membrane transport pathway had the highest average abundance (16.16%), and this increased at 35 °C and 40 °C. Generally, different temperatures had impacts on the physiological activity of A. cornea and the bacterial community in the substrate; therefore, the data presented herein should facilitate cultivation of A. cornea.

Short-Term Straw Returning Improves Quality and Bacteria Community of Black Soil in Northeast China

“…RDA results did not reveal significant positive correlation between soil OM content and soil microorganisms. Higher temperatures are expected to enhance soil bacteria activity, resulting in higher enzyme activity [31]. Enzyme activity peaked at the 2 nd or 3 rd sampling, likely due to higher temperature.…”

Microbial Community, Metabolic Potential and Seasonality of Endosphere Microbiota Associated with Leaves of the Bioenergy Tree Paulownia elongata × fortunei

“…However, it should be noted that experimentally different incubation temperatures might be a useful way to see how community function changes over the day/season. Many researchers indicate that temperature is an important environmental factor that can greatly influence the metabolic activity as well as the composition and diversity of the microbial community in different samples [ 62 , 63 , 64 ].…”

Beneficial bacterial-Auricularia cornea interactions fostering growth enhancement identified from microbiota present in spent mushroom substrate

“…The growth of both the mushroom and the mushroom growth-promoting bacteria can also be enhanced by available nutrients in certain environments such as those present in the mushroom spent substrate ( Carrasco et al, 2018 ; Wu et al, 2020 ; Upadhyay and Chauhan, 2022 ). In addition, certain mushroom growth-promoting bacteria can also adapt and function in various environments, e.g., a range of substrates and temperature ( Jemsi and Aryantha, 2017 ; Zhang et al, 2018 ).…”

“…One challenge currently facing mushroom farmers is that the fruiting body yields are sometimes unpredictable even when grown in a controlled environment. These inconsistent yields most likely stem from endogenous microorganisms that are present in the substrates during cultivation ( Zhang et al, 2018 ). Therefore, investigation of the microbial community that can affect mushroom growth can be beneficial for enhancing the mushroom yield.…”

“…The techniques have been used to explore the bacterial composition in the mushroom substrates and casing soil ( Oh et al, 2016 ; Benucci et al, 2019 ; Ke et al, 2019 ; Yang et al, 2019 ; Carrasco et al, 2020 ; Chen et al, 2022 ; Vieira and Pecchia, 2022 ). However, there is limited information on the substrate-associated bacterial communities and their relationship to the growth of Auricularia species ( Zhang et al, 2018 ). Therefore, in this study, we aimed to search for potential mushroom growth-promoting bacteria by comparison of bacterial communities in spent mushroom substrates obtained from high- and low-yield spent substrates via metagenomic approach.…”