Influence of temperature on volatile fatty acid production and microbial community structure during anaerobic fermentation of microalgae

Cho, Hyun Uk; Kim, Young Mo; Choi, Yun‐Nam; Kim, Hye Gyeong; Park, Jong

doi:10.1016/j.biortech.2015.03.009

Cited by 58 publications

(25 citation statements)

References 32 publications

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“…Taking into account the COD contribution of each VFA in the anaerobic effluents, psychrophilic and mesophilic digestions ranged the same COD conversion efficiency (COD‐VFA/COD in = 45–48% at pH 7.5, Table ). This value was considerably higher than that reported (10%) by other authors who investigated the digestion of a nontreated microalgae mixture at 35°C and pH 7 . When pH 5.5 was evaluated, similar percentage was obtained at psychrophilic conditions (COD‐VFA/COD in = 48%), whereas the lowest conversion was attained in thermophilic digestions regardless of pH (34 and 37% for pH 5.5 and 7.5, respectively) (Table ).…”

Section: Resultsmentioning

confidence: 51%

“…This value was considerably higher than that reported (10%) by other authors who investigated the digestion of a nontreated microalgae mixture at 35 C and pH 7. 15 When pH 5.5 was evaluated, similar percentage was obtained at psychrophilic conditions (COD-VFA/COD in = 48%), whereas the lowest conversion was attained in thermophilic digestions regardless of pH (34 and 37% for pH 5.5 and 7.5, respectively) ( Table 1). This trend was opposite to the pattern described by Cho el al., (2015).…”

Section: Effect Of Initial Ph and Temperature On Ad Of Protease Pretrmentioning

confidence: 73%

“…Moreover, acidogenic metabolic pathways are affected by digestion pH . Likewise, temperature, another easily tunable operational parameter, affects the growth and enzymatic activities of the anaerobic microbiome . Nevertheless, many aspects of VFAs production by AD remain unclear and in‐depth investigations to fully understand how to maximize parameters such as productivity, yields and composition are needed.…”

Section: Introductionmentioning

confidence: 99%

“…14 Likewise, temperature, another easily tunable operational parameter, affects the growth and enzymatic activities of the anaerobic microbiome. 15 Nevertheless, many aspects of VFAs production by AD remain unclear and in-depth investigations to fully understand how to maximize parameters such as productivity, yields and composition are needed. The most innovative point of the research presented herein is the use of a new feedstock, such as microalgae, for VFAs production instead of the most conventional AD devoted to biogas production.…”

Section: Introductionmentioning

confidence: 99%

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Volatile fatty acids production from protease pretreated Chlorella biomass via anaerobic digestion

Magdalena

Tomás‐Pejó

Ballesteros

et al. 2018

Biotechnology Progress

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Volatile fatty acids (VFAs) produced via anaerobic digestion (AD) are regarded as a low cost production process of building blocks of interest for the chemical industry. In this study, VFAs and methane production were assessed in batch reactors at different temperature ranges (psychrophilic 25°C, mesophilic 35°C, thermophilic 50°C) and different pH values (5.5 and 7.5) using protease pretreated Chlorella sp. biomass as substrate. Acetic acid and propionic acid were the most abundant products (up to 73% of the total VFAs) during the first days independently of the conditions. VFAs concentration decreased over time as methane was produced after a lag phase of 7–10 days. Results showed that best conditions for VFAs production were mesophilic temperature ranges (35°C) at neutral initial pH values (7.5), and psychrophilic temperature ranges (25°C) at low initial pH values (5.5) which resulted in a conversion of the initial COD into VFAs of 48%, respectively. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1363–1369, 2018

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Section: Resultsmentioning

confidence: 51%

Section: Effect Of Initial Ph and Temperature On Ad Of Protease Pretrmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Volatile fatty acids production from protease pretreated Chlorella biomass via anaerobic digestion

Magdalena

Tomás‐Pejó

Ballesteros

et al. 2018

Biotechnology Progress

View full text Add to dashboard Cite

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“…Genera Paenibacillus, Pseudomonas, Terrisporobacter, Actinomyces, Geofilum, Lachnoclostridium, Cryseobacterium, Oscillibacter, Acetivibrio, and Parabacteroides were found in lower abundances among core microbes (Nelson et al, 2011;Shida et al, 2012;Lee et al, 2013;Yan et al, 2013;Cho et al, 2015;Pap et al, 2015;Sun et al, 2016;Gagliano et al, 2017;Lemos et al, 2017;Nakajima et al, 2017;Neshat et al, 2017;Tian et al, 2017) (Supplementary Table 2).…”

Section: The Core Microbial Communitymentioning

confidence: 99%

Characterization of Core Microbiomes and Functional Profiles of Mesophilic Anaerobic Digesters Fed With Chlorella vulgaris Green Microalgae and Maize Silage

et al. 2019

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Microalgal biomass is an alternative feedstock for biogas production although its C/N ratio is usually lower than optimal, therefore co-fermentation is recommended. Biogas production from photoautotrophically grown Chlorella vulgaris (C. vulgaris) biomass (240 mL CH 4 g oTS −1 ) and co-fermentation with maize silage (330 mL CH 4 g oTS −1 ) has been studied in semi continuous laboratory biogas fermenters. Maize silage control yielded 310 mL CH 4 g oTS −1 . The microbial community and the read-based functional profiles, derived from these data, were examined during the process by using next-generation metagenome Ion Torrent sequencing technology. The read-based core microbiome consisted of 92 genera from which 60 abundant taxa were directly associated with the microbial methane producing food chain. The data-set was also analyzed in a genome-based approach. Sixty-five bins were assembled, 52 of them belonged in the core biogas producing genera identified by the read-based metagenomes. The read-based and genome-based approaches complemented and verified each other. The functional profiles indicated a variety of glycoside hydrolases. Substantial rearrangements of the methanogen functions have also been observed. Co-fermentation of algal biomass and plant biomass can be carried out for an extended period of time without process failure. The microbial members of the inoculum are well-conserved, feedstock composition changes caused mostly relative abundance alterations in the core microbiome.

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Organic Leachate and Biogas Utilization in Outdoor Microalgae Cultivation Under Alkaline Conditions at Pilot-Scale

Velasco

Franco‐Morgado

Saldivar

et al. 2023

Waste Biomass Valor

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The performance of an outdoor pilot-scale system consisting of a high rate algal pond interconnected to an absorption bubble column and seeded with a microalgal-bacterial consortium is presented. Biogas and organic leachate (from food waste anaerobic hydrolysis) were used as sources of CO2 and nutrients for the consortium, respectively. The removal efficiencies for CO2 and H2S from biogas were 80.0% and 99.9%, respectively, provoking a methane content in the utilized biogas of approximately 55 vol% for anaerobically-produced biogas and 90 vol% for a prepared gaseous mixture. The molecular oxygen produced by microalgae photosynthesis induced undesirable cumulation of O2 in utilized biogas up to 5%vol. The organic leachate was diluted in an alkaline mineral medium (15:1 v/v), and this rich-nutrient mixture was fed to the system at a hydraulic retention time of 138 d. The biomass productivity applying organic leachate and anaerobically-produced biogas was 2.6 g m−3 d−1, nine times lower than biomass productivity achieved when only mineral salt medium was fed, suggesting the existence of limited nutrient bioavailability. Microalgal-bacterial community shifts evaluated through molecular and morphological methods showed that a robust and stable photosynthetic population was maintained throughout the entire operation of the system, formed mainly by Picochlorum sp., Pseudanabaena sp., Spirulina sp., and Nitzschia sp., as well as heterotrophic bacteria and some microalgae predators. The valued by-products after waste utilization were clean and highly calorific biogas besides microalgae biomass, where the commercialization of the latter would determine the economic viability of biogas and leachate utilization. Graphical Abstract

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Influence of temperature on volatile fatty acid production and microbial community structure during anaerobic fermentation of microalgae

Cited by 58 publications

References 32 publications

Volatile fatty acids production from protease pretreated Chlorella biomass via anaerobic digestion

Volatile fatty acids production from protease pretreated Chlorella biomass via anaerobic digestion

Characterization of Core Microbiomes and Functional Profiles of Mesophilic Anaerobic Digesters Fed With Chlorella vulgaris Green Microalgae and Maize Silage

Organic Leachate and Biogas Utilization in Outdoor Microalgae Cultivation Under Alkaline Conditions at Pilot-Scale

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