Influence of Teratogenic Factors on Mouse 39hoxGene Expression

Kojima, Takao; Takahashi, Nobutaka

doi:10.1271/bbb.90378

Cited by 6 publications

(4 citation statements)

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“…RNA was isolated from mouse whole embryos (E10.75) using TRIzol reagent (Invitrogen, Carlsbad, CA). Semi-quantitative RT-PCR was performed as described in a previous report, 7) and expression levels were plotted relative to housekeeping gene Rplp0. For quantitative RT-PCR (qRT-PCR), first-strand cDNA was generated from up to 1 mg the total RNA using PrimeScriptÔ RT Master mix (Takara, Shiga, Japan) following the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

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Teratogenic Factors Affect Transcription Factor Expression

Kojima

Asano

Takahashi

2013

Bioscience, Biotechnology, and Biochemistry

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Chemical compounds are produced every day, many with adverse effects on human health, and hence it is vital to predict the risks to humans simply, rapidly, and accurately. Teratogens have a serious impact on fetal development. This has been studied mainly by phenotypic analysis of experimental animals. However, since phenotypes can vary within different species, we established a new evaluation system based on our recent finding that teratogens influence Hox gene expression in mice. Similarly to the Hox gene expression changes, the expression patterns of several transcription factors involved in development, including the Dlx, Irx, Sall, and T-box families, were altered after 6 h of exposure to retinoic acid (RA) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The expression changes in Dlx4, Dlx6, Irx5, Sall2, Sall3, Sall4, Tbx10, and Tbx22 were linked to teratogen-induced phenotypes, and our results indicate that expression changes in developmental transcription factors can help to predict teratogenic risk.

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Section: Methodsmentioning

confidence: 99%

“…7) These two factors caused similar phenotype, such as craniofacial y To whom correspondence should be addressed. Tel/Fax: +81-3-5841-5118; E-mail: antkhs@mail.ecc.u-tokyo.ac.jp abnormalities.…”

mentioning

confidence: 99%

Teratogenic Factors Affect Transcription Factor Expression

Kojima

Asano

Takahashi

2013

Bioscience, Biotechnology, and Biochemistry

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“…It is known that some Hox genes (such as Hoxc6 ) are responsive to estrogen [Garcia-Gasca and Spyropuolos, 2000] fore, it is likely that Hox genes would also be responsive to endocrine disrupting compounds. Kojima and Takahashi [2009] analyzed the expression changes of the 39 Hox genes in tetrachlorodibenzo-p-dioxin (TCDD)-retinoic acid (RA)-exposed mouse embryos. The authors identified that both teratogenic agents differentially affected the expression of Hox genes, but unlike RA, TCDD showed a broad repression of all Hox loci.…”

Section: Hox Genes Teratogenesis and Environmental Influencementioning

confidence: 99%

Hox Genes in Reptile Development, Epigenetic Regulation, and Teratogenesis

Martín-del-Campo

Sifuentes-Romero

García‐Gasca

2018

Cytogenet Genome Res

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Reptiles are ancestral organisms presenting a variety of shapes, from the elongated vertebral column of the snake to the turtle dorsalized ribs or retractile neck. Body plans are specified by a conserved group of homeobox-containing genes (Hox genes), which encode transcription factors important in cell fate and vertebral architecture along the anteroposterior axis during embryonic development; thus, dysregulation of these genes may cause congenital malformations, from mild-sublethal to embryonic-lethal. The genetic pool, maternal transfer, and environmental conditions during egg incubation affect development; environmental factors such as temperature, moisture, oxygen, and pollution may alter gene expression by epigenetic mechanisms. Thus, in this review, we present information regarding Hox genes and development in reptiles, including sex determination and teratogenesis. We also present some evidence of epigenetic regulation of Hox genes and the role of the environment in epigenetic modulation of gene expression. So far, the evidence suggests that the molecular instructions encoded by Hox genes to build a snake, a lizard, or a turtle represent the interplay between genome and epigenome after years of evolution, with occasional environmentally induced molecular mistakes leading to abnormal body shapes.

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“…In the present report, we focused on the expressional changes of the Hox cluster genes in embryos exposed to teratogenic factors because their aberrant expression lead to various morphological defects and the affected animals have close similarities (Kojima and Takahashi, 2009). …”

Section: Hox-ra/dioxinmentioning

confidence: 99%