Influence of TGF-β1 on the expression of BSP, DSP, TGF-β1 receptor I and Smad proteins during reparative dentinogenesis

Hwang, Yun-Chan; Hwang, In-Nam; Oh, Won-Mann; Park, Joo-Cheol; Lee, Dong-Seol; Son, Ho-Hyun

doi:10.1007/s10735-007-9148-8

Cited by 81 publications

(63 citation statements)

References 27 publications

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“…Regarding the regenerative process after tooth injuries such as cavity preparation and pulpal capping following pulp exposure, newly differentiated or surviving odontoblast-like cells express Dsp protein in addition to the immunoreaction in the tertiary dentin (14,25), suggesting that this protein is a reliable marker for regenerated odontoblasts and tertiary dentin. However, immunohistochemistry for Dsp is unable to determine the up-regulation of Dsp production in the afflicted odontoblasts, because Dsp protein has already been accumulated in their cytoplasm.…”

Section: Molar At Week 3 (Table 2)mentioning

confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day-to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.Dentin is a hard connective tissue that forms the bulk of the tooth, and it is a bone-like matrix characterized by multiple closely packed dentinal tubules that traverse its entire thickness and contain the cytoplasmic processes of odontoblasts, which are responsible for the formation and maintenance of the dentin. The cell bodies of the odontoblasts are aligned along the inner aspect of the dentin, beneath a layer of predentin, where they also form the peripheral boundary of the dental pulp (35). The odontoblasts are terminally differentiated ectomesenchymal cells that synthesize several collagenous and non-collagenous proteins (NCPs) (45). The morphologically discernible differentiation of the odontoblast begins with the dental papilla cells adjacent to the inner enamel epithelium (7). The dental papilla cells adjoining the acellular zone rapidly enlarge and elongate to become preodontoblasts first and then odontoblasts as their cytoplasm increases in volume to contain increasing amounts of proteinsynthesizing organelles. The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or

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Section: Molar At Week 3 (Table 2)mentioning

confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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“…Dental pulp cells can contribute to this process, and dentin ECM proteins actively promote and control mineralization of collagen fibers and apatite crystal growth during conversion of predentin to dentin (Butler et al, 2003). One ECM protein of notable importance is dentin sialophosphoprotein (DSPP), which undergoes cleavage to DPP (dentin phosphoprotein) and DSP (dentin sialoprotein), is associated with dentin mineralization (Papagerakis et al, 2002), and is expressed by odontoblast-like cells underlying newly formed reparative dentin Hwang et al, 2008).…”

Section: Introduction Dmentioning

confidence: 99%

TNF-α Promotes an Odontoblastic Phenotype in Dental Pulp Cells

et al. 2009

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“…It has been found that BSP binding to HA and collagen may promote bone mineralization [Hunter and Goldberg, 1993;Baht et al, 2008]. While BSP has been identified in porcine, rat and human dentin, its role in dentin mineralization is still unknown [Chen et al, 1993;Boukpessi et al, 2008;Huang et al, 2008;Hwang et al, 2008].…”

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confidence: 99%

Increased Matrix Metalloproteinase-2 and Bone Sialoprotein Response to Human Coronal Caries

Boushell

Nagaoka²,

Yamauchi

2011

Caries Res

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Background: It has been suggested that host matrix metalloproteinase-2 (MMP-2) present in dentin may be involved in caries progression, however, its response to caries is not known. Bone sialoprotein (BSP) has been implicated in dentin mineralization and MMP-2 modulation. Objective: To identify and compare the distribution of MMP-2 and BSP in healthy human coronal dentin and those with early caries. Methods: Freshly extracted 3rd molars and premolars with and without early caries were fixed, demineralized and subjected to immunohistochemistry using a monoclonal anti-MMP-2 antibody and monoclonal anti-BSP antibody with an avidin-biotin complex method. Immunoreactivity was visualized with 3,3′-diaminobenzidine substrate and observed under light microscopy. Results: Immunohistochemical analysis revealed that MMP-2 and BSP are not detected in the tubule lumens of healthy dentin. However, intense immunoreactivity for MMP-2 and BSP was detected in association with the full length of the caries-affected dentinal tubules. The MMP-2 and BSP at the dentino-enamel junction appeared unaltered. Conclusion: The results indicate that MMP-2 and BSP may be actively secreted by odontoblasts in response to carious insult. MMP-2 and BSP accumulation in the caries-affected dentinal tubules may indicate their potential involvement in the host defense mechanism which results in calcification of regions affected by the carious process.

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Influence of TGF-β1 on the expression of BSP, DSP, TGF-β1 receptor I and Smad proteins during reparative dentinogenesis

Cited by 81 publications

References 27 publications

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

TNF-α Promotes an Odontoblastic Phenotype in Dental Pulp Cells

Increased Matrix Metalloproteinase-2 and Bone Sialoprotein Response to Human Coronal Caries

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