Influence of the adipose derived hormone resistin on signal transducer and activator of transcription factors, steroidogenesis and proliferation of Leydig cells

Jean, Stéphanie; Landry, David; Daigle, Mikella; Martin, Luc J.

doi:10.1016/s2305-0500(13)60038-x

Cited by 6 publications

(5 citation statements)

References 35 publications

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“…Since Leydig cells produce resistin in low concentrations, they may control their own proliferation in an autocrine or paracrine manner (Roumaud & Martin, 2015). Indeed, exposure to small concentrations of resistin (10 ng/mL), corresponding to a normal physiological condition, contributes to increased proliferation of MA-10 Leydig cells (Jean et al, 2012). In addition, Sertoli cells may also contribute to Leydig cells proliferation by secreting resistin (Nogueiras et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Insulin resistance and atherosclerosis have been extensively studied, making a strong relation between obesity and increased morbidity. Recently, a direct relationship between obesity and lower steroids production and infertility has been discussed (Jean et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, resistin, among the other adipose derived hormones, might play a role in steroidogenic genes regulation in Leydig cells. A research has shown that resistin might influence steroidogenic genes expression and Leydig cells function (Jean et al, 2012). It has been reported that resistin has no effect on progesterone production, despite an increase in nuclear translocation of STAT factors and unexpected 3',5'-cyclic adenosine monophosphate (cAMP) dependent increase in synthesis of steroidogenic acute regulatory protein and cholesterol side-chain cleavage enzyme (Jean et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…A research has shown that resistin might influence steroidogenic genes expression and Leydig cells function (Jean et al, 2012). It has been reported that resistin has no effect on progesterone production, despite an increase in nuclear translocation of STAT factors and unexpected 3',5'-cyclic adenosine monophosphate (cAMP) dependent increase in synthesis of steroidogenic acute regulatory protein and cholesterol side-chain cleavage enzyme (Jean et al, 2012). In addition, exposure to normal doses of resistin could have beneficial effects on Leydig cell function, as it contributes to increase the viability and proliferation of these cells (Jean et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that resistin has no effect on progesterone production, despite an increase in nuclear translocation of STAT factors and unexpected 3',5'-cyclic adenosine monophosphate (cAMP) dependent increase in synthesis of steroidogenic acute regulatory protein and cholesterol side-chain cleavage enzyme (Jean et al, 2012). In addition, exposure to normal doses of resistin could have beneficial effects on Leydig cell function, as it contributes to increase the viability and proliferation of these cells (Jean et al, 2012). Resistin expression has not been reported in cattle testicles until now, therefore the aim of this research was to localization of resistin in testicular tissue of bull by immunohistochemistry.…”

Section: Introductionmentioning

confidence: 99%

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Expression of resistin in testis and epididymis of bulls

Mohammadi¹,

Shokrollahi²

2019

Journal of Livestock Science

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The resistin is an insulin-antagonizing factor that also plays a regulatory role in inflammation, immunity, food intake, and gonadal function. Although adipose tissue is the primary source of resistin, it is also expressed in other tissues, including the testis. This study was aimed to investigate the immunohistochemical (IHC) localization of resistin in testicular tissues of Holstein bulls. Mouse monoclonal antibody anti-resistin as primary antibody and rabbit anti-mouse IgG Horseradish Peroxidase (HRP) as secondary antibody were used. Samples of testis were collected from four bulls aged of 1.5 to 2 years old, and preserved in 10% formalin for posterior inclusion in paraffin. Histological sections with 5 micron in thickness were prepared for IHC. Immunoreactions were assessed for spermatogenic cells, Leydig cells, Sertoli cells and epididymis. Our results showed that the resistin is expressed in the testis. Immunoreactions were observed in Sertoli cells, Leydig cells, spermatogonia cells and cytoplasm of ciliated cells of the epididymis. But, the intensity of staining in Leydig cells and epididymis was very weak in comparison with other cells. Probably, resistin had autocrine effect in testis, therefore it could be involved in spermatogenesis and sperm motility.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of resistin in testis and epididymis of bulls

Mohammadi¹,

Shokrollahi²

2019

Journal of Livestock Science

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Steroidogenic genes expressions are repressed by high levels of leptin and the JAK/STAT signaling pathway in MA-10 Leydig cells

et al. 2017

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The adipose tissue is an important endocrine organ secreting numerous peptide hormones, including leptin. Increased circulating levels of leptin, as a result of hormonal resistance in obese individuals, may contribute to lower androgen production in obese males. However, the molecular mechanisms involved need to be better defined. Androgens are mainly produced by Leydig cells within the testis. In male rodents, activation of the leptin receptor modulates a cascade of intracellular signal transduction pathways which may lead to regulation of transcription factors having influences on steroidogenesis in Leydig cells. Thus, as a result of high leptin levels interacting with its receptor and modulating the activity of the JAK/STAT signaling pathway, the activity of transcription factors important for steroidogenic genes expressions may be inhibited in Leydig cells. Here we show that Lepr is increasingly expressed within Leydig cells according to postnatal development. Although high levels of leptin (corresponding to obesity condition) alone had no effect on Leydig cells' steroidogenic genes expression, it downregulated cAMP-dependent activations of the cholesterol transporter Star and of the rate-limiting steroidogenic enzyme Cyp11a1. Our results suggest that STAT transcriptional activity is downregulated by high levels of leptin, leading to reduced cAMP-dependent steroidogenic genes (Star and Cyp11a1) expressions in MA-10 Leydig cells. However, other transcription factors such as members of the SMAD and NFAT families may be involved and need further investigation to better define how leptin regulates their activities and their relevance for Leydig cells function.

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