Influence of the age and sex on respiratory burst of human monocytes

Alvarez, Eloísa; Marı́a, Consuelo Santa

doi:10.1016/0047-6374(96)01763-0

Cited by 20 publications

(10 citation statements)

References 16 publications

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“…Our observations also indicate that, in addition to the known effects of gender and age (10–12, 20, 21), a racial background may also have an important influence in determining the oxidative burst activity in neutrophils and monocytes. It remains to be elucidated if the observed individual characteristics or the gender and race‐dependent differences in oxidative burst activity have an impact on the inflammatory response under pathological conditions.…”

Section: Resultssupporting

confidence: 57%

“…It is not known whether decreased (or augmented) responsiveness of oxidative burst is also manifested in a parallel decrease (or augmentation) in cell adhesion molecule expression. Furthermore, whereas ample evidence indicates that gender and age have major influences on leukocyte responses and on the host response to injury and infection (10–12), the potential influence of ethnic background has not been investigated. Therefore, the aims of the study were to delineate the relationship between oxidative burst activity and expression of the CD11b adhesion molecule in neutrophils and monocytes from a representative pool of young healthy volunteers.…”

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confidence: 99%

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Relationship between oxidative burst activity and CD11b expression in neutrophils and monocytes from healthy individuals: Effects of race and gender

et al. 2001

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Oxidative burst activity and the expression of adhesion molecules have been used as indicators of leukocyte activation status. The aim of the study was to delineate the relationship of oxidative burst activity and the expression of adhesion molecules in neutrophils and monocytes from a pool of healthy volunteers (n ‫؍‬ 96). We also tested the potential role of gender and a racial background in the individual response differences. Basal and phorbol myristate acetate (PMA)-stimulated oxidative burst and CD11b expression were determined using dihydrorhodamine 123 and phycoerythrin ( Key terms: neutrophils; monocytes; cell activation; flow cytometry; humanDetermination of oxidative burst and the expression of adhesion molecules by flow cytometry technique have been widely used in the assessment of leukocyte functional status (1,2). It has been accepted that oxidative burst activity and the expression of cell adhesion molecules parallel the activation status of neutrophils and macrophages. Elevated oxidative burst activity or CD11b expression indicates cell activation whereas decreased responses reflect downregulated cellular functions. The value of these tests has been well established in a variety of disease processes. Marked differences have been observed in leukocyte CD11b expression (3-6) and oxidative burst response (7-9) between patients and healthy volunteers. However, empirical observations have suggested that considerable variability exists among individuals in the degree of oxidative burst and CD11b expression in leukocytes. It is not known whether decreased (or augmented) responsiveness of oxidative burst is also manifested in a parallel decrease (or augmentation) in cell adhesion molecule expression. Furthermore, whereas ample evidence indicates that gender and age have major influences on leukocyte responses and on the host response to injury and infection (10 -12), the potential influence of ethnic background has not been investigated. Therefore, the aims of the study were to delineate the relationship between oxidative burst activity and expression of the CD11b adhesion molecule in neutrophils and monocytes from a representative pool of young healthy

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Section: Resultssupporting

confidence: 57%

mentioning

confidence: 99%

Relationship between oxidative burst activity and CD11b expression in neutrophils and monocytes from healthy individuals: Effects of race and gender

et al. 2001

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“…There is limited data describing the monocyte capacity to produce reactive oxygen species upon stimulation with PMA. This function may be affected by age and sex, as demonstrated previously although in a different read-out system (17). However, the study of Alvarez and Santa Marina (17) included subjects aged 25-55 and lacks data for individuals before puberty and teenagers.…”

Section: Discussionmentioning

confidence: 80%

“…This function may be affected by age and sex, as demonstrated previously although in a different read-out system (17). However, the study of Alvarez and Santa Marina (17) included subjects aged 25-55 and lacks data for individuals before puberty and teenagers. Due to the small sample size, we cannot speculate whether the considerable differences in measured fluorescence observed between young subjects in our study are a reflection of the hormonal or any other developmental variability in those individuals.…”

Section: Discussionmentioning

confidence: 80%

Flow Cytometric Assay of Respiratory Burst Capacity in Peripheral Blood Monocytes of Gaucher Type 1 Patients

Rodić¹,

Popadić²,

Suvajdzic-Vukovic³

et al. 2014

Journal of Medical Biochemistry

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Background: There is an apparently increased tendency toward infections in patients with Gaucher disease, possibly due to defective neutrophil function rather than a decreased neutrophil count. Since macrophages are the main cell type affected in Gaucher disease, our aim was to determine the contribution of these cells to the susceptibility of Gaucher patients to infection by studying the respiratory burst capacity of peripheral blood monocytes. Methods: The study was performed in eleven Gaucher type 1 patients and eleven sex and age matched control subjects by measuring peripheral blood monocytes’ respiratory burst capacity using flow cytometry. The respiratory burst capacity was measured as dihydrorhodamine-123 median fluorescence in patients and respective controls. Results: There was no statistical difference in the median fluorescence among the patients and respective controls (p>0.05) after phorbol 12-myristate 13-acetate stimulation. Also, statistical difference was not reached among patients treated with enzyme replacement therapy at the time and those untreated. Conclusions: Flow cytometry might represent a more accurate and more reliable measure of respiratory burst compared to the methods of other researchers. Respiratory burst disturbance in monocytes does not seem to contribute to increased susceptibility to infection in Gaucher patients.

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“…Innate immunity, which consists of phagocytic cells and natural killer (NK) cells, continues to function reasonably well 79 . Most studies indicate that the chemotaxis, adherence, and phagocytic ability of monocytes, Mφ, and polymorphonuclear cells (PMNs) are not affected by aging, 38 although a decrease in the respiratory burst of monocytes, PMN production of reactive oxygen species, and chemotaxis have been reported in elderly subjects compared to young subjects 80 …”

Section: Age‐associated Dysregulation Of the Immune System: Implicatimentioning

confidence: 99%

Low zinc status: a new risk factor for pneumonia in the elderly?

Barnett¹,

Hamer²,

Meydani³

2010

Nutrition Reviews

121

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Zinc may be a new risk factor for pneumonia in the elderly. In this special article, we reviewed the magnitude of the problem of pneumonia (its prevalence, morbidity and mortality) in the elderly, its etiology, and the dysregulation of the immune system associated with increasing age. In addition, we presented evidence from the literature, including work we did recently, that low zinc status (commonly reported in the elderly) impairs immune function, decreases resistance to pathogens, and is associated not only with increased incidence and duration of pneumonia, increased use and duration of antimicrobial treatment, but also with increased overall mortality in the elderly. Inadequate stores of zinc might therefore be a risk factor for pneumonia in the elderly. Randomized, double blind, controlled studies are needed to determine the efficacy of zinc supplementation as a potential low cost intervention to reduce morbidity and mortality due to pneumonia in this vulnerable population. KeywordsPlease provide between three and five key words or phrases

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Influence of the age and sex on respiratory burst of human monocytes

Cited by 20 publications

References 16 publications

Relationship between oxidative burst activity and CD11b expression in neutrophils and monocytes from healthy individuals: Effects of race and gender

Relationship between oxidative burst activity and CD11b expression in neutrophils and monocytes from healthy individuals: Effects of race and gender

Flow Cytometric Assay of Respiratory Burst Capacity in Peripheral Blood Monocytes of Gaucher Type 1 Patients

Low zinc status: a new risk factor for pneumonia in the elderly?

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