2017
DOI: 10.21521/mw.5675
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Influence of the age of rats treated with the sodium salt of glutamate acid on the reactivity of astroglia of the infundibular nucleus

Abstract: The aim of the study was the immunohistochemical evaluation of the impact of the age of animals treated with the sodium salt of glutamic acid on the behaviour of astrocytes of the infundibular nucleus (IN). Immunohistochemical peroxidase-antiperoxidase reactions were conducted on brain sections of 10-day-old (I) and 63-day-old (II) rats treated s.c with monosodium glutamate (MSG) in a dose of 4g/kg b.w. for three consecutive days. The staining was performed using specific antibodies against glial fibrillary ac… Show more

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“…At the same time, negative control of the immunohistochemical reactions by omission of the primary antibody was performed. The specificity of the primary antibody was confirmed on the rat brain sections in our earlier study [47]. The stained sections were analyzed and microphotographs were obtained in Olympus BX40 microscope connected to the digital Olympus Color View III camera (Olympus, Tokyo, Japan).…”
Section: Methodsmentioning
confidence: 95%
“…At the same time, negative control of the immunohistochemical reactions by omission of the primary antibody was performed. The specificity of the primary antibody was confirmed on the rat brain sections in our earlier study [47]. The stained sections were analyzed and microphotographs were obtained in Olympus BX40 microscope connected to the digital Olympus Color View III camera (Olympus, Tokyo, Japan).…”
Section: Methodsmentioning
confidence: 95%