The Late Pleistocene ami Holocene evolution of the estuaries in !he Gulf of Cadiz is interpreted for the first time using drill cores, logs, trenches, and 38 new radiocarbon data, and the results compared with lhe she!f The Odie!, Tinto and Guadalete Rivers deposited conglomerales during a highstand lhat did not reach the present sea level dated al ca. 25-30 ka (Isotopic Stage (IS) 3), corresponding lo a relatively humid period in the area. Rivers incised these coarse-grained deposits during the last main lowsland at ca. 18 ka, when sea level dropped to -120 m and the coastline lay 14 km seawards from !he presenL The erosional surface is a sequence boundary and the f100ding slIrface of tl1e postglacial eustatic rise, overlain by !he valley fill deposits of the transgressive and highstand phases of the last fourth-and fif!h-order depositional sequences recognised in !he shelf The first marine int1uence in the estuaries during the transgression occurs at -25/ -30 m at ca. 10,000 years BP. According to tossil assemblages, the transgressed basins changed fmm brackish to more open marine as !he sea rose lIntil ca. 6500 years BP, when it reached !he maximum f100ding ami the sandy estuarine barriers ceased to retrograde toward the muddy central basins. Then, the rate of eustatic rise decreased drastically, and !he estuarine filling followed a two-fold pattem govemed by the progressive change from vertical accretion to lateral (centripelal) progradation. At ca. 4000 years BP the t1uvial input surpassed !he already negligible rate of rise, causing partial emergence of tidal f1ats and spit barriers in the largely filled estuarine basins. Prevalence of coastal progradation upon vertical accretion al ca. 2400 years BP caused accelerated expansion of tidal t1ats and rapid growth of the sandy barriers. Further changes since the 16th century ref1ect widespread anthropic impacts.