Influence of the Content in Transcription Factors on the Phenotype of Mouse Hepatocyte-like Cell Lines (mhAT)

Three-dimensional polarization sensitizes hepatocytes to Fas/CD95 apoptotic signalling

“…Hepatocytes form organoids in a Matrigel-based 3D culture The mhAT3F hepatocyte cell line, established from a mouse with liver-targeted production of SV40 large T antigen, retains a pattern of gene expression resembling that of normal hepatocytes (Levrat et al, 1993). The cells grow in monolayer cultures as flattened clusters (Fig.…”

“…In the present study, we have used mhAT3F, a differentiated murine hepatocyte cell line (Antoine et al, 1992;Levrat et al, 1993), to assess the possible links between the cells' polarity and function with their sensitivity to apoptosis induced by stimulation of death receptors. Contrary to the reported resistance to apoptosis of polarized mammary epithelium (Weaver et al, 2002), strong polarization of hepatocytes in a three-dimensional culture diminished NF-κB activation and restored the cells' in vivo sensitivity to the stimulation of the Fas pathway.…”

Role of the GLUT 2 Glucose Transporter in the Response of the L-type Pyruvate Kinase Gene to Glucose in Liver-derived Cells

“…In the hepatoma cell lines de- scribed to date, neither GLUT 2 nor glucokinase were ever reported to be significantly expressed, being replaced by GLUT 1 and insulin-independent hexokinase isoforms, mainly HK 1 (8,16). We have previously established and characterized various new hepatoma cell lines, some of which express GLUT 2 but not glucokinase and yet are responsive to glucose (2,19,20). Hepatoma cells that express mostly GLUT 1 exhibit a glucose-independent L-PK gene expression, whereas GLUT 2(ϩ) cell lines express the L-PK gene poorly under glucose-free conditions and strongly under glucose conditions.…”

“…Hepatocytes were obtained from transgenic animals of different ages with either normal, fetal (mhPKTf3), or tumoral (mhPKT, mhAT1, and mhAT3) livers and established in culture as described previously (19 -21). About 30 mhAT1-and mhAT3-derived subclones were isolated using different selective media, particularly containing fructose as the only carbohydrate source (19,20). Lines mhPKTf3F1 and F2 were subcloned from * This work was supported in part by grants from La Ligue Nationale contre le Cancer and l'Association de Recherche sur le Cancer.…”

“…Hepatocytes were obtained from fetal or adult transgenic animals with either normal (mhPKTf3) or tumoral livers (mhPKT, mhAT1, and mhAT3 cell lines) and established in culture as described previously (19,20). Each line was derived from a single clone.…”