Influence of the cultivation conditions of grape microclones in vitro on their survival in vivo

Zelenianska, N.M.; Samofalov, M.O.

doi:10.32851/2226-0099.2022.127.8

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“…For the reproduction of grapes with the use of in vitro tissue and organ culture, modified nutrient media based on Murashige and Skoog medium are used. Its composition includes macro-and microsalts, calcium chloride, iron chelate, vitamins, indoleacetic acid, 6-benzylaminopurine, sucrose, agar (Zelenianska, 2022). According to the results of the research in this paper, for better growth and development of grape microclones of rootstock and technical varieties, the course of physiological processes in the tissues of leaves and shoots, optimal is the Murashige and Skoog nutrient medium with a minimum content of phytohormones in the composition: 0.3 mg/l IAA, 0.2 mg/l BAP.…”

Section: Figure 3 the Content Of Dry Matter And Water In The Tissues ...mentioning

confidence: 99%

Influence of nutrient media on the physiological parameters of grape microclones

Zelenianska¹,

Ishchenko²,

Samofalov³

2023

Scientific Horizons

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Successful rooting of grape microclones in uncontrolled environmental conditions (in vivo) depends on the level of resistance, which is formed at the stage of their passage and growth in vitro. An important role in this case is played by indicators of the water regime of the vegetative mass. The purpose of this study was to get acquainted with the results of determining the water-holding capacity, the intensity of transpiration of grape microclones in vitro and to establish the share of their influence on the adaptation potential in vivo. Biotechnological, laboratory, vegetation, and mathematical and statistical research methods were used in this study. The obtained results showed that to optimize the physiological processes in the tissues of the leaves and shoots of grape microclones, to increase their viability under in vivo conditions, it is advisable to cultivate them in vitro on structured nutrient media (MS + agroperlite, MS + vermiculite, MS + agroperlite + vermiculite) with the content of phytohormones IAA – 0.2 mg/l, and 6-BAP – 0.3 mg/l. Structured nutrient media contributed to an increase in water retention capacity and a decrease in the intensity of transpiration of tissues of leaves and shoots of microclones of both technical and rootstock varieties. During 60 minutes of research, from 0.006 g to 0.034 g of water evaporated from microclones of technical varieties, from 0.003 g to 0.053 g from microclones of rootstock varieties, respectively. Transpiration intensity (after 10 min) decreased by 1.7-1.8 times. On the control Murashige and Skoog nutrient medium, plants evaporated a larger amount of water during the corresponding period of time: from 0.006 g to 0.079 g (technical varieties) and from 0.008 g to 0.086 g (rootstock varieties); the intensity of transpiration was higher. After cultivation of grape microclones on structured nutrient media, they were characterized by a higher content of dry matter in the tissues of leaves and shoots (14.6-15.0%) and better survival rates in vivo (76.3-98.5%, at 58.5-65.2% in control). The reliability of the results obtained is confirmed by the results of multivariate analysis of variance. The obtained results expand the understanding of dynamic changes in the indicators of the water regime of the vegetative mass of grape microclones in vitro, their influence on plant survival under in vivo conditions

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Section: Figure 3 the Content Of Dry Matter And Water In The Tissues ...mentioning

confidence: 99%

Influence of nutrient media on the physiological parameters of grape microclones

Zelenianska¹,

Ishchenko²,

Samofalov³

2023

Scientific Horizons

View full text Add to dashboard Cite

show abstract

Section: Figure 3 the Content Of Dry Matter And Water In The Tissues ...mentioning

confidence: 99%

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2023

Scientific Horizons

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Рекомендовано до друку та поширення через мережу Інтернет Вченою радою Поліського національного університету (протокол № x від xx 2023 року) Свідоцтво про державну реєстрацію друкованого засобу масової інформації серії КВ № 24997-14937 ПР Науковий журнал включено до категорії А Переліку наукових фахових видань України, у яких можуть публікуватися результати дисертаційних робіт на здобуття наукових ступенів доктора та кандидата ветеринарних, економічних, сільськогосподарських і технічних наук зі спеціальностей: 051 -Економіка; 071 -Облік і оподаткування; 072 -Фінанси, банківська справа та страхування; 073 -Менеджмент; 075 -Маркетинг; 076 -Підприємництво, торгівля та біржова діяльність; 101 -Екологія; 133 -Галузеве машинобудування; 201 -Агрономія; 202 -Захист і карантин рослин; 203 -Садівництво та виноградарство; 204 -Технологія виробництва і переробки продукції тваринництва; 205 -Лісове господарство; 206 -Садово-паркове господарство; 208 -Агроінженерія; 211 -Ветеринарна медицина (наказ МОН України № 1166 від 23 грудня 2022 р.) Журнал представлено у міжнародних наукометричних базах даних, репозитаріях та пошукових системах: Інституційний репозитарій Поліського національного університету, AGRICOLA, CAB Abstracts and Global Health (CABI), Open Academic Journals Index (OAJI), Scopus, Index Copernicus, Реєстр наукових видань України, Національна бібліотека України імені В. І. Вернадського, Crossref, Directory of Open Access Journals (DOAJ) Адреса редакції: Поліський національний університет б-р Старий,

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Influence of the cultivation conditions of grape microclones in vitro on their survival in vivo

Cited by 2 publications

References 0 publications

Influence of nutrient media on the physiological parameters of grape microclones

Influence of nutrient media on the physiological parameters of grape microclones

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