Influence of the culture medium on the fructosyltransferase production

Maiorano, Alfredo Eduardo; Silva, E.S.; Piccoli, Rosane A. M.; Ottoni, Cristiane Angélica; Guilarte, B.; Cuervo, Raúl A.; Moreira, R.; Rodrígues, Mario

doi:10.1016/j.nbt.2009.06.137

Cited by 7 publications

(6 citation statements)

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“…According to Maiorano et al (2009), the maximum production of FTase using A. oryzae IPT-301 occurred in the range of pH 4.5 to 5.0, while the maximum biomass production was verified for fermented broths with pH 5.0. A. oryzae IPT 301 is a producer of acid where A and B represent the levels of the factors temperature (ºC) and pH, respectively, while…”

Section: Experimental Design and Statitical Analysismentioning

confidence: 99%

“…In a bioreactor operated in a one stage process, using the whole cells of the microorganism FOS production reached 0.64 ± 0.02 g FOS .g initial sucrose -1 . In our previous group experiments (Cuervo-Fernandez et al, 2007;Maiorano et al, 2009;Ottoni et al, 2012) the FTase produced by Aspergillus oryzae IPT-301 presented high transfructosylation activities when concentrated sucrose solutions were used as substrate, which consequently led to high production of FOS. Despite its excellent performance, there is no previous research in the literature which aimed to study the influence of the reaction media temperature, pH and sucrose concentration on Aspergillus oryzae IPT-301 FTase.…”

Section: Introductionmentioning

confidence: 97%

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SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION

Cunha

Ottoni

Morales

et al. 2019

Braz. J. Chem. Eng.

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Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 °C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 °C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.

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Section: Experimental Design and Statitical Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION

Cunha

Ottoni

Morales

et al. 2019

Braz. J. Chem. Eng.

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“…Also, it is noteworthy that the properties of FTase vary also in microorganisms depending on media parameters mostly the carbon source. Despite the fact that quite a number of microorganisms produce FTase, just a few have the potential for biotechnological or industrial production such as in the synthesis of fructooligosaccharides [15].…”

Section: Introductionmentioning

confidence: 99%

“…Fructooligosaccharides (FOS) are oligosaccharides derived from the addition of a fructose moiety to a sucrose molecule which can be carried out by the actions fructosyltransferase and/or β-fructofuranosidase [24], [15]. Both enzymes are classified as FOS-producing enzymes.…”

Section: Introductionmentioning

confidence: 99%

Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8

Ademakinwa

Ayinla

Agboola

2017

Journal of Genetic Engineering and Biotechnology

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Strain improvement of a low fructosyltransferase-producing Aureobasidium pullulans NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide and ethyl methane sulfonate. The wild-type and mutant strain were distinguished using Random amplified polymorphic DNA PCR and DNA fingerprinting analysis. Plackett-Burman and Box Behnken design were statistical tools used to determine important media parameters and optimization, respectively. Phenotypically and genetically, the new improved strain was different from the wild-type. The most important media parameters from PDB influencing fructosyltransferase production were ammonium chloride, sucrose and yeast extract at p < 0.05. Some significant parameters obtained with the BBD exhibited quadratic effects on FTase. The F values (35.37 and 32.11), correlation coefficient (0.98 and 0.97) and the percent coefficient of variation (2.53% and 2.40%) were obtained for extracellular and intracellular FTase respectively. The validation of the model in the improved strain resulted in an overall 6.0 and 2.0-fold increase in extracellular and intracellular FTase respectively compared to the wild-type.A relatively low FTase-producing strain of Aureobasidium pullulans NAC8 was enhanced for optimum production using a two-pronged approach involving mutagenesis and statistical optimization. The improved mutant strain also had remarkable biotechnological properties that make it a suitable alternative than the wild-type.

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“…Fructosyltransferases (EC 2.4.1.9) are enzymes that catalyze the transfer of fructose units from a sucrose molecule to another molecule of sucrose or to fructose in a process that yields higher amounts of fructooligosaccharide (FOS) units (Maiorano et al, 2009). FOSs have also been termed as fructose oligomers; they have a terminal glucose residue that is linked to fructose via glycosidic bond (β-2,1).…”

Section: Introductionmentioning

confidence: 99%

Preparation, characterization and optimization of cross-linked fructosyltransferase aggregates for the production of prebiotic fructooligosaccharides

Ademakinwa¹,

Ayinla²,

Omitogun³

et al. 2018

bta

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In this study, the preparation, characterization, and evaluation of the cross-linked enzyme aggregates (CLEAs) of fructosyltransferase isolated from the mutant-type Aureobasidium pullulans NAC8 for the production of fructooligosaccharides (FOS) with prebiotic properties were investigated. The reaction conditions were optimized statistically by using response surface methodology. CLEAs were characterized by using both photo-microscopy and scanning electron microscopy energy-dispersive X-ray spectroscopy (SEM-EDX). The secondary structure of the protein was predicted by using Fourier transform infrared spectroscopy (FTIR). FOSs produced as a result of the biotransformation of sucrose was quantified by using a chemometric analysis of FTIR spectral data. The prepared FOSs were investigated for the prebiotic effects under anaerobic conditions using a standard strain of Lactobacillus sp. Following were the statistically determined, optimum conditions for the production of FOSs: 5% (v/v) glutaraldehyde, pH 5.5, and a temperature of 32EC. The values of R 2 , F value, adjusted precision, and coefficient of variation were found to be 0.94, 11.50, 9.34, and 35.34%, respectively. Validation of the model resulted in the reusability of the CLEAs in six reaction cycles as it retained 70% of the residual activity. The SEM-EDX and photomicrographic analysis showed that the sizes of CLEAs varied, which ranged between 10 and 40 μm. Aggregation leads to the formation of a higher number of beta sheets in the CLEAs than that in the free enzyme. The total FOSs produced by the CLEAs was around 57% (w/v). A. pullulans FOS supplemented to the medium had a stimulatory effect on the growth of bi dobacteria compared with the control medium. The growth curves of Lactobacillus sp. reached the plateau phase after 24 h of cultivation with FOS. The CLEA of fructosyltransferase catalyzed the biocatalysis of sucrose to FOSs, a process with a biotechnological importance as prebiotics.

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Influence of the culture medium on the fructosyltransferase production

Cited by 7 publications

References 0 publications

SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION

SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION

Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8

Preparation, characterization and optimization of cross-linked fructosyltransferase aggregates for the production of prebiotic fructooligosaccharides

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