Influence of the Environment on the Morphological and Biochemical Characteristics of Different Aspergillus niger Wild Type Strains

Abstract

The present work studied the differences in accumulation, transformation and volatilization of different heavy metals ions on molecular and macromorphological features of Aspergillus niger wild type strains. Four different strains of A. niger (An) were used. Three strains (An-P, An-N, An-S) were isolated from acid and ultra acid mining regions with higher concentration of As and Sb. The fourth strain (An-G) was used as the comparative one. Environmental burden strongly affected biochemical, macro and micromorphological characteristics of studied strains. The RAMP profiles showed 90 % similarity among the studied strains. The strain An-S showed its own characteristic RAMP profile, different to the others ones. Analyzed strains can be clustered into two groups on the basis of the changes in gene expression and morphological parameters. Differences were found in both acid b-1,3-glucanases and peroxidases. Main quantitative and qualitative differences by A-PAGE and SDS-PAGE were registered for proteins with Mr * 50; 34; 28-27 and 11 kDa. Presence of living mutants of A. niger strains in old environmental burden indicate on the adaptation and mutation processes of soil microorganisms from the point of long-term effect.

Alkaline Technosol contaminated by former mining activity and its culturable autochthonous microbiota

“…spinulosum , Penicillium sp.). Ubiquitous Aspergillus and Penicillium species were identified in different substrates such as extreme environments with heavy metal contamination ( Amich et al., 2010 , Ezzouhri et al., 2009 , Šimonovičová et al., 2013a , Šimonovičová et al., 2013b , Rasool and Irum, 2014 ), biological soil crusts ( Kubátová et al., 2002 , Kubátová and Prášil, 2008 , Bates et al., 2010 , Bates et al., 2012 ) and they have very high potential for bioremediation ( Lietão, 2009 , Ren et al., 2009 , Dugal and Gangawane, 2012 ) and can be used for heavy metal bioleaching ( Anjum et al., 2010 , Zeng et al., 2015 ). Minimal humus content is a limit factor for expansion of Mucormycotina species, except of Mortierella alpina in the sample 3.…”

“…The substrate surface is occasionally moss-grown ( Krokusová, 2005 ). Such a substrate clearly represents an extreme environment for microorganisms ( Roadcap et al., 2006 , Levyk et al., 2007 , Wang et al., 2011 , Gostinčar and Turk, 2012 , Gostinčar et al., 2012 , Šimonovičová et al., 2013a , Šimonovičová et al., 2013b , Šimonovičová et al., 2016 ). Anyway, those are well-adapted to specific conditions at the level of their micromorphological structures and metabolic activities as well.…”

Responses of Aspergillus niger to selected environmental factors

“…Studied strains were isolated from mixed culture of soil microscopic filamentous fungi. Pure cultures of all strains were cultivated in an incubator at 25 °C for 5-7 days on SAB and identified according to micromorphological features and PCR analyses (Šimonovičová et al 2013;Jesenák et al 2015).…”

Comparative Analysis of Aspergillus oryzae with Normal and Abnormal Color Conidia

“…A. oryzae with anomalous color conidium was collected from A. oryzae AS 3.951 culture, suspended and serially diluted, and streaked on additional new plates until homogenous colonies were observed (Fig. 1), due to the spontaneous mutation [7,8].…”

“…Profiles of the extracellular proteins of A. oryzae with anomalous color conidium were obtained by SDS-PAGE electrophoresis [3,7], and the main protein bands were excised and identified by MALDI TOF/MS analysis. As shown in Fig.…”