Influence of the ester carbonyl oxygens of lecithin on the permeability properties of mixed lecithin-cholesterol bilayers

Schwarz, Frank; Paltauf, Fritz

doi:10.1021/bi00639a001

Cited by 48 publications

(16 citation statements)

References 33 publications

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“…At lower growth temperature, the levels of stearic acid and cis-vaccenic acid in position sn-1 of PE were markedly increased with a concomitant decrease in the level of 18:0 DMA, indicating that the depressed level of alkylacyl and alkenylacyl subclasses of PE were compensated for by the increase in levels of diacylPE containing these C18 fatty acids in position sn-1. Judging from experiments with monolayers and bilayers, ether bonding has been considered to be less polar than carbonyl ester bonding (18,19). Moreover, PE containing monoenoic acid at position sn-1 have been shown to have a disordering effect on biomembranes (20,2 l).…”

Section: Discussionmentioning

confidence: 99%

Effects of growth temperature on the fatty acid composition of the free‐living nematodeCaenorhabditis elegans

Tanaka

Ikita

Ashida

et al. 1996

Lipids

110

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The effects of growth temperature on the fatty acid compositions of the phosphatidylcholine (PC), phosphatidylethanolamine (PE), and total lipid (TL) fractions of the free-living nematode Caenorhabditis elegans were investigated. A reduction in growth temperature from 25 to 15 degrees C caused the proportions of eicosapentaenoic acid (20:5n-3) to increase from 23.6 to 32.5% in the PC, from 7.4 to 10.8% in the PE, and from 12.9 to 19.9% in the TL fractions. Conversely, the levels of dihomo-gamma-linolenic acid (20:3n-6) and arachidonic acid (20:4n-6) in these phospholipid fractions and the TL fraction both decreased with decreasing growth temperature. Analysis of the positional distribution of fatty acids in the PC fraction revealed that the change in the composition of C20 polyunsaturated fatty acid was obvious in position sn-2. Lowering the growth temperature induced an increase in the level of the diacyl subclass of PE from 58% at 25 degrees C to 71% at 15 degrees C, with a concomitant decrease in the levels of the alkylacyl and alkenylacyl subclass of PE of C. elegans. These changes observed in the phospholipids of C. elegans might be one mechanism for adaptation to low temperature.

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Section: Discussionmentioning

confidence: 99%

Effects of growth temperature on the fatty acid composition of the free‐living nematodeCaenorhabditis elegans

Tanaka

Ikita

Ashida

et al. 1996

Lipids

110

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“…The structural and most of the functional studies were performed by using a 24:1 (w/w) mixture of (Schwarz & Paltauf, 1977), and milky suspensions of phospholipid/cholesterol mixtures were formed in Triton X-100. The suspensions did not clarify even if the detergent concentration was raised to 5%.…”

Section: Protein Compositionmentioning

confidence: 99%

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

Wolosin¹

1980

Biochemical Journal

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A short procedure for the isolation of band-3 protein, the protein responsible for anion exchange in erythrocytes, in a reasonable degree of purity was developed. Using this protein preparation and a novel procedure for membrane-protein reconstitution, vesicles displaying the basic features of the anion-exchange system of the erythrocyte were obtained. The reconstitution procedure is based on slow direct removal of Triton X-100 from aqueous lipid/detergent solutions. According to the composition of the reconstitution medium, either small single-walled or large multi-walled vesicles are obtained. The procedure conserves protein properties well, as is revealed by the similarity of the rates of SQ42-exchange in erythrocytes and reconstituted vesicles when corrected for the relevant volumes. A number of functional features of the exchange system were studied and compared with those of the native membrane.

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“…To study the small amounts of glycerolipids extracted from biological samples, several analytical methods have been pursued 3–5. Mass spectrometry (MS) is known to be an important analytical tool for the structural studies of compounds extracted from trace amounts of biological samples.…”

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confidence: 99%

Structural determination of cyclitol derivatives by fast‐atom bombardment tandem mass spectrometry

Gil

Seo

Kim

et al. 2006

Rapid Comm Mass Spectrometry

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Three cyclitol derivatives were isolated from the marine sponge Sarcotragus sp. by reversed-phase high-performance liquid chromatography and analyzed by fast-atom bombardment mass spectrometry (FAB-MS). Their structural elucidation was carried out with FAB tandem mass spectrometry (FAB-MS/MS). FAB-MS spectra produced a significant abundance of the sodium adducts [M+Na]+ and [M+2Na-H]+ from a mixture of m-NBA and NaI. In addition, trifluoroacetylation of the cyclitol derivatives was used for confirmation of the presence of the cyclitol ring. High abundance [M-5H+5CF3CO+Na]+ ions were observed in the FAB-MS spectra of the trifluoroacetyl-cyclitol derivatives. Collision-induced dissociation (CID) of the [M+Na]+ ions produced diverse product ions via a series of dissociative processes. Charge-remote fragmentation (CRF) patterns of [M+Na]+ ions were very useful for the identification of product ions which are characteristic for the cyclitol ring and long hydrocarbon chains substituted at the glycerol backbone. Moreover, the CID-MS/MS spectra of the [M+Na]+ ions yielded characteristic product ions at m/z 53, 83, 113, 155 and 171 for the cyclitol moiety, and at m/z 213, 229 and 245 for the glycerol backbone attached to the cyclitol ring.

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Influence of the ester carbonyl oxygens of lecithin on the permeability properties of mixed lecithin-cholesterol bilayers

Abstract: The passive diffusion of Na+, Cl-, and glucose

Cited by 48 publications

References 33 publications

Effects of growth temperature on the fatty acid composition of the free‐living nematodeCaenorhabditis elegans

Effects of growth temperature on the fatty acid composition of the free‐living nematodeCaenorhabditis elegans

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

Structural determination of cyclitol derivatives by fast‐atom bombardment tandem mass spectrometry

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