Influence of the Explant Type on the Phenotypic Changes in Micropropagated Plants of Artichoke

Cadinu, Marco; Repetto, A.; Frau, Antonio Maria; Beneventi, S.; Meloni, Sandro

doi:10.17660/actahortic.2004.660.54

Cited by 5 publications

(3 citation statements)

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“…Alterations in global methylation patterns in in vitro culture have been reported [82], and reflect the adaptation process of cells to a different environment which includes the response to signals that may trigger switches in the developmental program. So far, in ‘Spinoso sardo’ factors like the number of subculture cycles, the sampling period and the relative size of offshoots (containing meristem apex) have been investigated to study their potential correlation with the off-type phenotype emergence [43,83]. Results showed that the sampling period did not influence the number of aberrant phenotypes [83].…”

Section: Discussionmentioning

confidence: 99%

“…Clones of the AGRIS3 selection of ‘Spinoso sardo’, were obtained by AGRIS agency (S’Appassiu Research Center, Cagliari, Italy) via in vitro culture of meristematic apices in growth chambers at 22 °C for 20 days under long day (16-h light, 8-h dark) photoperiods, as previously described [43]. Micropropagated plantlets were transferred in soil and grown in growth chambers under the same conditions for 20–30 days, then transplanted in open field in Cagliari (S’Appassiu Research Center), Italy (39°18 ′N, 8°55 ′E, 17 m a.s.l.).…”

Section: Methodsmentioning

confidence: 99%

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Analysis of DNA Methylation Patterns Associated with In Vitro Propagated Globe Artichoke Plants Using an EpiRADseq-Based Approach

Cerruti

Comino

Acquadro

et al. 2019

Genes

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Globe artichoke represents one of the main horticultural species of the Mediterranean basin, and ‘Spinoso sardo’ is the most widespread and economically relevant varietal type in Sardinia, Italy. In the last decades, in vitro culture of meristematic apices has increased the frequency of aberrant plants in open-field production. These off-type phenotypes showed highly pinnate-parted leaves and late inflorescence budding, and emerged from some branches of the true-to-type ‘Spinoso sardo’ plants. This phenomenon cannot be foreseen and is reversible through generations, suggesting the occurrence of epigenetic alterations. Here, we report an exploratory study on DNA methylation patterns in off-type/true-to-type globe artichoke plants, using a modified EpiRADseq technology, which allowed the identification of 2,897 differentially methylated loci (DML): 1,998 in CG, 458 in CHH, and 441 in CHG methylation contexts of which 720, 88, and 152, respectively, were in coding regions. Most of them appeared involved in primary metabolic processes, mostly linked to photosynthesis, regulation of flower development, and regulation of reproductive processes, coherently with the observed phenotype. Differences in the methylation status of some candidate genes were integrated with transcriptional analysis to test whether these two regulation levels might interplay in the emergence and spread of the ‘Spinoso sardo’ non-conventional phenotype.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Analysis of DNA Methylation Patterns Associated with In Vitro Propagated Globe Artichoke Plants Using an EpiRADseq-Based Approach

Cerruti

Comino

Acquadro

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…Micropropagation of globe artichoke is an alternative method for production of large-scale healthy, high quality and uniform vegetative material. The use of in vitro propagation of globe artichoke, as a way of improving its rate of multiplication, was reported in several studies focused on the medium composition, growth regulators, genotypes, and the type of explants (Cadinu et al, 2004;Tavazza et al, 2004;Grando et al, 2011;Iapichino, 2013). In vitro propagation of globe artichoke was primary utilised for a few spring cultivars, but it was more difficult for autumn ones due to loss of earliness in a significant part of micropropagated plants (Tavazza et al, 2004).…”

Section: In Vitro Propagationmentioning

confidence: 99%

Application of modern agronomic and biotechnological strategies to valorise worldwide globe artichoke (Cynara cardunculus L.) potential - an analytical overview

Grabowska

Caruso

Mehrafarin

et al. 2018

Italian Journal of Agronomy

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The globe artichoke [Cynara cardunculus L. var. scolymus (L.) Fiori], an ancient vegetable originated in the Mediterranean Basin, is currently cultivated in many regions of the world under a perennial cycle or as an annual crop, with the first method being more widespread globally. The growing importance of globe artichoke as modern functional food as well as a source of pharmaceuticals has raised new issues that all producers have to face; hence the necessity of contemporaneous development of new centres of production and new technologies application in traditional regions of growing which can complement the global market. This review is focused on development of globe artichoke technology of production in recent several years which meet the diversified requirements of global and local markets. We considered the recent literature to highlight specific applications of modern farming practices and plant breeding along with genetic variation to globe artichoke production system as well as to postharvest management in order to enhance the value added of this commodity. The latter targets are mainly addressed to particular regions of the world and they are based on farmers knowledge, equipment, scale and methods of production, processing, final market. Our reports are focused on sustainable and environmentally friendly methods which can improve the profitability of production as well as product’s quality and quantity traits. We discussed the balanced mineral application which can precisely affect the yield chemical composition, attractiveness and shelf life of globe artichoke heads as well as create the opportunities to attain standardised by-products, valuable on the market of health and convenient food. Further topics were developed, such as introduction of seed propagation, intercropping, grafting, flowering induction, postharvest treatments as linked to different regions and conditions of production. Precise selection of modern management practices was recognised as a main goal to fulfil the requirements of local and global market for fresh, processed and new potential globe artichoke products.

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Globe Artichoke Tissue Culture and Its Biotechnological Application

Comino

Moglia

Repetto

et al. 2019

Compendium of Plant Genomes

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Influence of the Explant Type on the Phenotypic Changes in Micropropagated Plants of Artichoke

Cited by 5 publications

References 0 publications

Analysis of DNA Methylation Patterns Associated with In Vitro Propagated Globe Artichoke Plants Using an EpiRADseq-Based Approach

Analysis of DNA Methylation Patterns Associated with In Vitro Propagated Globe Artichoke Plants Using an EpiRADseq-Based Approach

Application of modern agronomic and biotechnological strategies to valorise worldwide globe artichoke (Cynara cardunculus L.) potential - an analytical overview

Globe Artichoke Tissue Culture and Its Biotechnological Application

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