Influence of the genotype of Holstein breed bulls-producers on the sodium content in the blood serum of sons

Chemical elements, as structural components of the body, influence physiological processes and functions of the organism, growth, productive and reproductive traits, resistance and others. In this regard, the present study aimed to evaluate the effect of different calcium concentrations in the wool of Holstein sires on the semen quality characteristics. The studies were conducted in conditions of Leningrad and Vologda regions on Holstein sires, 3–5 years old (n = 40). Calcium concentration in wool was determined by atomic emission and mass spectrometry (AES–ICP and MS–ICP). According to the results of this analysis and comparison with previously developed reference intervals, we formed four groups of sires: I – level of Ca concentration less than 25th percentile, II and IV – within 25–75th percentile (physiological norm: 1597–2926 mg/kg) and III – more than 75th percentile. As a result of this distribution, the Ca concentration in the wool of animals of Group II was higher by 75.9 % compared to Group I; in turn, individuals of Group IV exceeded Group III – by 27.9 %. According to the results of semen quality characteristics evaluation, it was found that in the group of sires with Ca level within the previously developed reference intervals, the concentration of spermatozoa was higher by 15.3 % and their activity – by 0.17 points in fresh semen in comparison with the group with its reduced level (below the 25th percentile). High Ca content (above the 75th percentile) in wool contributed to a 9.0 % decrease in the concentration of spermatozoa compared to its average values. Thus, the calcium concentration, assessed by its level in the fur, affects the concentration and activity of sperm. It is necessary to evaluate it to improve the quality characteristics of sperm systematically.

unclassified

Evaluation of the effect of calcium concentration in the wool of sires on se- men quality characteristics

Sleptsov,

Miroshnikov,

Frolov

2024

Influence of mother’s genotype on the concentration and variability of triiodothyro- nine in landrace pigs

Tarasenko,

Konovalova,

Osadchuk

et al. 2024

Self Cite

One of the leading roles in the hormonal system of the body of pigs in terms of functional significance is played by the thyroid gland, which, through the hormone triiodothyronine, has a complex effect on all systems, organs and physiological processes while controlling the metabolism and energy in the animal’s body. The impact of maternal genotype on the concentration and variability of triiodothyronine in complete siblings of Landrace pigs was studied. For the study, blood serum samples were taken from 33 pigs using the vacuum method from the jugular vein. Blood samples were taken according to generally accepted practices. The studies were carried out in the laboratory of the biochemistry of the Novosibirsk State Agrarian University using the technique of competitive enzyme immunoassay with a set of reagents “Steroid ELISA-triiodothyronine” to quantify the concentration of triiodothyronine in blood serum. Enzyme immunoassay was performed on a Thermo Scientific Multiskan FC analyser. In pig breeding, the content of heavy metals in water, soil, feed, organs and tissues was constantly monitored. The concentration of chemical elements did not exceed the MPC level. Statistical processing of experimental data was carried out using standard methods of descriptive statistics and the statistical programming language or data analysis environment RStudio (using the functions: descrstats, summary, sd, read.table, write. table, aes). The strength of the influence of the genotype of mothers of the Landrace breed on the triiodothyronine content in complete siblings in the conditions of Western Siberia was established, which amounted to 4.6 %. In some mothers’ offspring, the triiodothyronine level was 1.9 and 1.8 times higher than in others. Two clusters of mothers were identified according to the concentration of triiodothyronine in complete siblings, which included three groups of mothers with an average level of 2.26 nmol/l and five groups with the attention of 1.30 nmol/l. The reference limits of the triiodothyronine level in Landrace pigs in the conditions of Western Siberia were established.

The impact of the paternal genetic makeup on the copper concentration in the livers of offspring in cattle

Konovalova

2024

he copper content in the liver of the progeny of four Holstein breed bulls was investigated. Research was done in Kemerovo, Russia. The identical dietary and lodging conditions were established for all groups of offspring after their birth. The copper concentration was studied using atomic emission spectrometry on the SOLAR M6 series instrument in the Analytical Center of the Institute of Geology and Mineralogy, named by V.S. Sobolev. The determination of the heavy metal content in water, soil, and fodder within the dairy cattle zoning was conducted, with the result that the level did not exceed MAC. The normal distribution was determined by using the Shapiro-Wilk test. The Dunn’s test, coupled with Holm’s correction, was employed to evaluate the offspring of the four fathers. The influence of Holstein-bred bulls on copper deposition in offsprings was established. The amount of it in some bulls sons was higher than in others by 2.7 times (49.4 mg/kg). According to the Cu concentration in the liver, bulls were arranged in the following order: 2.7 : 2.0 : 1.3 : 1.0. The concentration of Cu in the liver of the offspring of the fathers was characterized by distinct phenotypic variations. The strength of influence (rw) of the father’s genotype on the variation of copper levels in the liver of sons was 25%. Two groups of fathers were identified with different levels of copper in the livers of their offspring: one group had levels of copper from 49.4 to 38.5 mg/kg, and the other group had levels from 27.6 to 18.2 mg/kg. The variability of copper content in the livers of the offspring of different fathers turned out to be low and ranged from 6.4% to 10%.