Influence of the Phytosynthesis of Noble Metal Nanoparticles on the Cytotoxic and Genotoxic Effects of Aconitum toxicum Reichenb. Leaves Alcoholic Extract

Șuțan, Nicoleta Anca; Vilcoci, Denisa Stefania; Fierăscu, Irina; Neblea, Angela Monica; Şuțan, Claudiu; Ducu, Cătălin; Soare, Liliana Cristina; Negrea, Denis; Avramescu, Sorin Marius; Fierăscu, Radu Claudiu

doi:10.1007/s10876-019-01524-9

Cited by 9 publications

(3 citation statements)

References 34 publications

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“…When comparing our results with the literature data, it can be noted that using the selected extracts, we obtained nanoparticles with dimensions comparable with those obtained using Raphanus sativus L. waste extracts [ 29 ], Asplenium scolopendrium L. leaves [ 46 ], Aconitum toxicum Reichenb. leaves [ 47 ], and A. toxicum rhizomes [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

et al. 2022

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With their phytoconstituents acting as reducing and capping agents, natural extracts can be considered a viable alternative for the obtaining of metallic nanoparticles. The properties of phytosynthesized nanoparticles are dependent upon size and morphology, which, in turn, can be tailored by adjusting different parameters of the phytosynthesis process (such as the extracts’ composition). In the present study, we aimed to evaluate, for the first time in the literature, the influence of the extraction method and extract concentration on the morphological and biological properties (antioxidant and antibacterial activity) of silver nanoparticles phytosynthesized using Echinacea pupurea L. extracts. The obtained results revealed that the use of the low-concentration Echinacea hydro-alcoholic extract obtained via classical temperature extraction led to the development of nanoparticles with the smallest dimensions (less than 10 nm), compared with the use of extracts obtained with higher concentrations and the extract obtained via the microwave method. The developed nanomaterials exhibited enhanced antioxidant effects (determined via the DPPH assay) and antimicrobial properties (against Escherichia coli and Candida albicans), compared with the parent extracts.

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Section: Discussionmentioning

confidence: 99%

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

et al. 2022

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“…Their results suggested a superior effect on the gastric cancer cells, the antiproliferative effect being mediated by nuclear condensation or DNA fragmentation. Vinay et al [92] used both cell lines and the Allium cepa assay for the evaluation of the cytotoxicity of phytosynthesized NPs, identifying antimitotic activity and chromosomal aberrations (chromosome-breaks, chromosome-stickiness, laggard chromosomes, and clumped chromosomes) that represented very good indicators of the genotoxic potential, thus making them a viable alternative for replacing cell lines in antitumoral studies [93]. Gold nanoparticles that were obtained by using green tea extract were also proved to possess antitumoral effects on different cell lines (human gastric adenocarcinoma, epithelial cervix adenocarcinoma, hepatocyte carcinoma and colorectal adenocarcinoma cells) [96].…”

Section: Antitumoral Applicationsmentioning

confidence: 99%

Phytosynthesized Metallic Nanoparticles—between Nanomedicine and Toxicology. A Brief Review of 2019′s Findings

et al. 2020

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Phytosynthesized nanoparticles represent a continuously increasing field of research, with numerous studies published each year. However, with the emerging interest in this area, the quality of the published works is also continuously increasing, switching from routine antioxidant or antimicrobial studies on trivial microbial lines to antibiotic-resistant strains or antitumoral studies. However, this increasing interest has not been not reflected in the studies regarding the toxicological effects of nanoparticles (NPs); this should be a subject of greatest interest, as the increasing administration of NPs in general (and phytosynthesized NPs in particular) could lead to their accumulation in the environment (soil, water and living organisms). The present review aims to present the most recent findings in the application of phytosynthesized NPs as antimicrobial and antitumoral agents, as well as the results regarding their toxicological potential.Materials 2020, 13, 574 2 of 32 in terms of their toxic potential towards target organisms (leading to their proposal as antimicrobial [4], antiviral [5] or cytogenotoxic agents [6]), their possible negative toxicity has not been thoroughly established. The present review aims to present the advances that have been recorded in the last year in the area of phytosynthesized nanoparticle applications and toxicity studies, as well to underline the shortcomings of the encountered approach. The literature review survey was performed on multiple databases (Scopus, Web of Science, ScienceDirect, SpringerLink, PubMed) and used the main keywords "nanoparticles" and "extract" (as the term "phytosynthesis" was not adopted by all the authors). From the returned results, only the works that were published in the last year (2019) were selected. Furthermore, a supplementary selection was performed by using keywords as "antimicrobial," "cytotoxicity," and "toxicology" (results presented in Figure 1). The results of this preliminary selection led to the return of approximately 1800 unique works (from all the databases). A preliminary validation was manually performed (by reading the keywords and abstract) in order to remove the "false-positive" results. The final validation was performed by reading the entire manuscript. The criteria for inclusion in the present review were: (i) the use of phytosynthesized nanoparticles that were obtained in the laboratory; (ii) the existence of analytical studies for the characterization of the nanoparticles; and (iii) in-depth applications reports. The review is structured in different chapters for each potential application that is related to the toxic effect of the metallic nanoparticles.

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“…The simple extracts were then mixed with the specific precursors in volume ratios of 1:1 and incubated for 24 h at room temperature (25°C). The colour change of the extracts after the addition of the 2 types of precursor solutions was noticeable within the first 2 min, which was a first confirmation of the formation of noble MNPs (Sutan et al 2019).…”

Section: Biogenic Synthesis Of Noble Metal Nanoparticles Mediated By ...mentioning

confidence: 61%

Biogenic synthesis of noble metal nanoparticles using Melissa officinalis L. and Salvia officinalis L. extracts and evaluation of their biosafety potential

et al. 2023

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In this study we targeted the noble metal nanoparticles (MNPs) biogenic synthesis capacity of two medicinal species with therapeutic potential, namely Melissa officinalis L. (lemon balm) and Salvia officinalis L. (sage), cultivated in Romania. Plant material was extracted by maceration, microwave assisted extraction (MAE) and ultrasound assisted extraction (UAE). Bright field scanning transmission electron microscopy and energy dispersive X-ray spectroscopy (BFSTEM-EDS) techniques were used in order to investigate particles shape, dispersion and chemical elemental analysis. The total polyphenol content for both simple extracts and nanostructured mixtures was determined using the Folin-Ciocalteu method and antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Identification and quantification of secondary metabolites of M. officinalis and S. officinalis were performed by ultra-high performance liquid chromatography (UHPLC). The Allium assay was used to evaluate the potential cytogenotoxic activity, for both simple and nanostructured phytochemical complexes, in the case of S. officinalis L. species being performed for the first time. Spherical shaped MNPs with diameters of about 20 nm were biosynthesised in lemon balm extracts. Larger AuNPs were phytosynthesized in sage extract obtained by UAE. Compared to the simple extracts, the antioxidant capacity as well as the amount of total polyphenols in the nanostructured extracts decreased, substantiating the involvement of bioorganic material in the reduction of metal ions. Low frequency of chromosomal aberrations corresponding to crude extracts and extracts supplemented with MNPs, suggest the cytoprotective, antigenotoxic, and safe use of these plant species as potential therapeutic forms in various diseases.

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Influence of the Phytosynthesis of Noble Metal Nanoparticles on the Cytotoxic and Genotoxic Effects of Aconitum toxicum Reichenb. Leaves Alcoholic Extract

Cited by 9 publications

References 34 publications

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

Phytosynthesis of Biological Active Silver Nanoparticles Using Echinacea purpurea L. Extracts

Phytosynthesized Metallic Nanoparticles—between Nanomedicine and Toxicology. A Brief Review of 2019′s Findings

Biogenic synthesis of noble metal nanoparticles using Melissa officinalis L. and Salvia officinalis L. extracts and evaluation of their biosafety potential

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