Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeruginosa PAO1, as revealed by transcriptome analysis

Burrowes, Elizabeth; Baysse, Christine; Adams, Claire; O’Gara, Fergal

doi:10.1099/mic.0.28324-0

Cited by 156 publications

(167 citation statements)

References 64 publications

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“…Expression was shown to be ninefold higher when the PAO1 and lasR/rhlR double mutant strains were compared (Schuster et al, 2003). A similar phenotype is seen in the post-transcriptional regulator rsmA mutant background (Burrowes et al, 2006).…”

Section: Gly-glusupporting

confidence: 49%

Genetic analysis of genes involved in dipeptide metabolism and cytotoxicity in Pseudomonas aeruginosa PAO1

et al. 2008

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The dipeptide transport operon in bacteria comprises genes for the transport and metabolism of amino acids and dipeptides, as well as haem and haem precursors such as aminolaevulinic acid. Such nutrient and mineral sources are vital for bacteria to survive in and colonize a range of niches. In silico analysis of the dipeptide transport systems in sequenced Pseudomonas species identified the presence of two genes in P. aeruginosa strains that were absent in other sequenced pseudomonads. These genes encode a putative metallopeptidase, PA4498, and a putative transcriptional regulator, PA4499. Proteomic profiling of wild-type PAO1 and a PA4499 mutant strain indicated that PA4499 negatively regulated the putative peptidase, PA4498. Transcriptional fusion analysis verified that expression of PA4498 (mdpA, metallo-dipeptidase aeruginosa) was negatively regulated by the downstream putative transcriptional regulator PA4499 (psdR, Pseudomonas dipeptide regulator). Transcriptional fusion analysis also showed that the dppABCDF operon was under the negative control of psdR. Functional genomic analysis of mdpA indicated that it is required for the metabolism of a range of dipeptides and that it contributes to the cytotoxicity of PAO1 on an epithelial cell line.

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Section: Gly-glusupporting

confidence: 49%

Genetic analysis of genes involved in dipeptide metabolism and cytotoxicity in Pseudomonas aeruginosa PAO1

et al. 2008

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“…In P. aeruginosa, RsmA assimilates sensory information and functions as a rheostat that permits a continuum of phenotypic responses (7,8). In the current study, we describe RsmF as a structurally distinct RsmA homolog whose discovery adds another level of complexity to posttranscriptional regulation in P. aeruginosa.…”

Section: Discussionmentioning

confidence: 97%

“…The CsrA homolog in Pseudomonas aeruginosa (RsmA) plays an important role in the regulation of virulence factors associated with acute and chronic infections (7)(8)(9). RsmA positively controls factors associated with acute infections including genes controlled by the cAMP/virulence factor regulator (Vfr) system, a type III secretion system (T3SS), and type IV pili (9).…”

mentioning

confidence: 99%

An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses inPseudomonas aeruginosa

Marden¹,

Diaz

Walton³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

184

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Members of the CsrA family of prokaryotic mRNA-binding proteins alter the translation and/or stability of transcripts needed for numerous global physiological processes. The previously described CsrA family member in Pseudomonas aeruginosa (RsmA) plays a central role in determining infection modality by reciprocally regulating processes associated with acute (type III secretion and motility) and chronic (type VI secretion and biofilm formation) infection. Here we describe a second, structurally distinct RsmA homolog in P. aeruginosa (RsmF) that has an overlapping yet unique regulatory role. RsmF deviates from the canonical 5 β-strand and carboxyl-terminal α-helix topology of all other CsrA proteins by having the α-helix internally positioned. Despite striking changes in topology, RsmF adopts a tertiary structure similar to other CsrA family members and binds a subset of RsmA mRNA targets, suggesting that RsmF activity is mediated through a conserved mechanism of RNA recognition. Whereas deletion of rsmF alone had little effect on RsmA-regulated processes, strains lacking both rsmA and rsmF exhibited enhanced RsmA phenotypes for markers of both type III and type VI secretion systems. In addition, simultaneous deletion of rsmA and rsmF resulted in superior biofilm formation relative to the wild-type or rsmA strains. We show that RsmF translation is derepressed in an rsmA mutant and demonstrate that RsmA specifically binds to rsmF mRNA in vitro, creating a global hierarchical regulatory cascade that operates at the posttranscriptional level.virulence | signal transduction | RsmY | RsmZ

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“…In the Gram-negative opportunistic pathogen Pseudomonas aeruginosa, RsmA is a pleiotropic post-transcriptional regulator that modulates the expression of w500 genes Burrowes et al, 2006). RsmA indirectly activates the expression of genes associated with the establishment of acute infections whilst repressing those implicated in the development of chronic infections ).…”

Section: Introductionmentioning

confidence: 99%

Complex autoregulation of the post-transcriptional regulator RsmA in Pseudomonas aeruginosa

2015

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RsmA is a post-transcriptional RNA-binding protein that acts as a pleiotropic global regulator of mRNAs in the opportunistic pathogen Pseudomonas aeruginosa. Upon binding to its target, RsmA impedes the translation of the mRNA by the ribosome. The RsmA regulon affects over 500 genes, many of which have been identified as important in the pathogenicity of P. aeruginosa. Whilst the regulatory function of RsmA is relatively well characterized, the genetic regulation of rsmA itself at the transcriptional and translational levels remains poorly understood. Here, we show that RsmA is capable of self-regulation through an unorthodox mechanism. This regulation occurs via direct interaction of the protein with an RsmA-binding site located in the early portion of its coding sequence. To the best of our knowledge this is the first report of such an unusual regulation in pseudomonads.

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Influence of the regulatory protein RsmA on cellular functions in Pseudomonas aeruginosa PAO1, as revealed by transcriptome analysis

Cited by 156 publications

References 64 publications

Genetic analysis of genes involved in dipeptide metabolism and cytotoxicity in Pseudomonas aeruginosa PAO1

Genetic analysis of genes involved in dipeptide metabolism and cytotoxicity in Pseudomonas aeruginosa PAO1

An unusual CsrA family member operates in series with RsmA to amplify posttranscriptional responses inPseudomonas aeruginosa

Complex autoregulation of the post-transcriptional regulator RsmA in Pseudomonas aeruginosa

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