Diesel combustion noise has become a crucial aspect for the engine manufacturers due to its impact on human health and influence on the customer purchasing decision. The interaction of the pressure waves after mixture self-ignition induces cavity resonances inside the combustion chamber. This complex phenomenon produces high-frequency pressure oscillations, hence traditional in-cylinder measurements do not provide enough information to characterise the in-cylinder acoustic field. In this paper, a numerical methodology is proposed for assessing the Diesel combustion as a noise source and to overcome measurement limitations. An optimisation procedure is also presented in order to determine the numerical calculation parameters, boundary conditions definition and initialization. Results show that local flow conditions at the start of combustion have a strong influence on the acoustic response of the in-cylinder noise source. These particular conditions are only achievable by the proposed methodology which considers entire engine cycle simulations with the complete cylinder domain. Therefore, traditional Computational Fluid Dynamic (CFD) approaches, such those used for predicting combustion stability or pollutant emissions, are not suitable for reproducing the physical mechanisms of noise generation and they cannot be used for acoustic purposes. The reliability of the proposed methodology to simulate the acoustic field accurately inside the combustion chamber has been validated by comparison with experiments.