Influence of the size and the morphology of ZnO nanoparticles on cell viability

Vasile, Otilia Ruxandra; Serdaru, Ioana; Andronescu, Ecaterina; Trușcă, Roxana; Surdu, Vasile–Adrian; Oprea, Ovidiu; Ilie, Andreia; Vasile, Bogdan Ştefan

doi:10.1016/j.crci.2015.08.005

Cited by 35 publications

(20 citation statements)

References 32 publications

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“…On the other hand, in the control sample ( Figure 2 C), no zinc oxide was formed, which indicates that only the specific combination of biologically active compounds from supernatant allows for effective ZnO NC formation. The difference between the ZnO NCs’ size estimated from the TEM and XRD analysis can be attributed to the fact that the particles are formed from more than one crystallite [ 40 , 41 ]. Furthermore, nanoparticles tend very often to create aggregates—therefore, the proper sample preparation steps are decisive [ 2 ].…”

Section: Resultsmentioning

confidence: 99%

“…No weight decline was observed at temperatures above 500 °C. Vasile et al [ 40 ] showed the good thermal stability of chemically synthesized ZnO nanocomposites to 200 °C, whereas Basha et al [ 73 ] described the green synthesis of nano-ZnO by using an alginate with thermal stability up to 160 °C. Based on these data, it can be concluded that biosynthesis allowed the formation of slightly less thermostable ZnO NCs, which is closely related to values reached by our group.…”

Section: Resultsmentioning

confidence: 99%

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Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

et al. 2020

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This research presents, for the first time, the potential of the Lactobacillus paracasei LC20 isolated from sweet whey as a novel, effective and accessible source for post-cultured ZnO nanocomposites synthesis. The obtained nanocomposites were subjected to comprehensive characterization by a broad spectrum of instrumental techniques. Results of spectroscopic and microscopic analysis confirmed the hexagonal crystalline structure of ZnO in the nanometer size. The dispersion stability of the obtained nanocomposites was determined based on the zeta potential (ZP) measurements—the average ZP value was found to be −29.15 ± 1.05 mV in the 7–9 pH range. The ZnO nanocomposites (NCs) demonstrated thermal stability up to 130 °C based on the results of thermogravimetric TGA/DTG) analysis. The organic deposit on the nanoparticle surface was recorded by spectroscopic analysis in the infrared range (FT-IR). Results of the spectrometric study exhibited nanostructure-assisted laser desorption/ionization effects and also pointed out the presence of organic deposits and, what is more, allowed us to identify the specific amino acids and peptides present on the ZnO NCs surfaces. In this context, mass spectrometry (MS) data confirmed the nano-ZnO formation mechanism. Moreover, fluorescence data showed an increase in fluorescence signal in the presence of nanocomposites designed for potential use as, e.g., biosensors. Despite ZnO NCs’ luminescent properties, they can also act as promising antiseptic agents against clinically relevant pathogens. Therefore, a pilot study on the antibacterial activity of biologically synthesized ZnO NCs was carried out against four strains (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa) by using MIC (minimal inhibitory concentration). Additionally, the colony forming units (CFU) assay was performed and quantified for all bacterial cells as the percentage of viable cells in comparison to a control sample (untreated culture) The nanocomposites were effective among three pathogens with MIC values in the range of 86.25–172.5 μg/mL and showed potential as a new type of, e.g., medical path or ointment formulation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

et al. 2020

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“…Noble metal-based nanoparticles (NPs) are widely used in biopharmaceutical applications [ 1 ] to develop innovative pharmaceutical forms. Silver NP (AgNP) synthesis, in particular, which uses plant extract as a reducing agent, is more advantageous over any other methods that use microorganisms; this process has received great attention in recent years [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract

et al. 2018

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The ability to orally administer silver nanoparticles (AgNPs) in enteric capsules implies a direct interaction with the colon microbiota. The in vitro effect provides a portrayal of the functional properties under in vivo conditions. The purpose of this study was to describe a green AgNP synthesis process, using aqueous extract from Lactarius piperatus mushroom, and to characterize the nanomaterial. We determined its antimicrobial and antioxidant effects in vitro in the microbiota of healthy individuals via the GIS1 system—a colon transit simulator. Per the quantitative polymerase chain reaction (qPCR) results, the antimicrobial properties of the AgNPs affected the initial share of different enteric species by decreasing the Bacteroides, Enterobacteriaceae, and Lactobacillus populations and favoring the Bifidobacterium group. The association between AgNPs and wild mushroom L. piperatus extract had a synergistic antibacterial activity against various pathogenic microorganisms while the mushroom extract reduced biofilm formation. Administration of AgNP maintained its constant antioxidant status, and it was correlated with a reduction in ammonium compounds. The physicochemical characterization of these NPs complemented their biochemical characterization. The maximum ultraviolet-visible spectroscopy (UV-VIS) absorbance was observed at 440 nm, while the Fourier transform infrared (FT-IR) spectrum reached a peak at 3296 cm–1, which was correlated with the high-performance liquid chromatographic analysis (HPLC). The major phenolic compound was homogentisic acid. The size (49 ± 16 nm in diameter) and spherical shape of the NPs were correlated with their biological effects in vitro.

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“…ZnO and TiO 2 nanoparticles are widely used not only in antimicrobial packaging but also in cosmetics and paints. ZnO is classified by FDA as GRAS and its toxicological effects are still under investigation, but some reports on ZnO nanoparticles are already alarming [ 276 , 277 , 278 ]. ZnO nanoparticles can pass the stomach and enter in the digestion process in intestine [ 279 ] and can induce hyperproliferation of malignant cells [ 280 ].…”

Section: Toxicity Studiesmentioning

confidence: 99%

Biodegradable Antimicrobial Food Packaging: Trends and Perspectives

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Ficai

et al. 2020

Foods

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This review presents a perspective on the research trends and solutions from recent years in the domain of antimicrobial packaging materials. The antibacterial, antifungal, and antioxidant activities can be induced by the main polymer used for packaging or by addition of various components from natural agents (bacteriocins, essential oils, natural extracts, etc.) to synthetic agents, both organic and inorganic (Ag, ZnO, TiO2 nanoparticles, synthetic antibiotics etc.). The general trend for the packaging evolution is from the inert and polluting plastic waste to the antimicrobial active, biodegradable or edible, biopolymer film packaging. Like in many domains this transition is an evolution rather than a revolution, and changes are coming in small steps. Changing the public perception and industry focus on the antimicrobial packaging solutions will enhance the shelf life and provide healthier food, thus diminishing the waste of agricultural resources, but will also reduce the plastic pollution generated by humankind as most new polymers used for packaging are from renewable sources and are biodegradable. Polysaccharides (like chitosan, cellulose and derivatives, starch etc.), lipids and proteins (from vegetal or animal origin), and some other specific biopolymers (like polylactic acid or polyvinyl alcohol) have been used as single component or in blends to obtain antimicrobial packaging materials. Where the package’s antimicrobial and antioxidant activities need a larger spectrum or a boost, certain active substances are embedded, encapsulated, coated, grafted into or onto the polymeric film. This review tries to cover the latest updates on the antimicrobial packaging, edible or not, using as support traditional and new polymers, with emphasis on natural compounds.

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Influence of the size and the morphology of ZnO nanoparticles on cell viability

Cited by 35 publications

References 32 publications

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

In Vitro Human Microbiota Response to Exposure to Silver Nanoparticles Biosynthesized with Mushroom Extract

Biodegradable Antimicrobial Food Packaging: Trends and Perspectives

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