Influence of the substrate's hydrophilicity on the <i>in vitro</i> Schwann cells viability

Soria, José Miguel; Martínez‐Ramos, Cristina; Bahamonde, Olga; Cruz, Dunia M. García; Salmerón-Sánchez, Manuel; Esparza, María Ángeles García; Casas, Caty; Guzmán, Mónica S.; Navarro, Xavier; Ribelles, J.L. Gómez; García‐Verdugo, José Manuel; Pradas, Manuel Monleón; Barcia, Juan A.

doi:10.1002/jbm.a.31297

Cited by 50 publications

(61 citation statements)

References 43 publications

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“…For PEA, a lower surface free energy and greater polar component than those previously published values [38,39] were obtained here; as explained, these discrepancies can be a side effect of the production procedure.…”

Section: On the Acrylates Interaction With Rad16-isupporting

confidence: 55%

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

et al. 2016

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Self-assembling peptides (SAP) are widely used as scaffolds themselves, and recently as fillers of microporous scaffolds, where the former provides a cell-friendly nanoenvironment and the latter improves its mechanical properties. The characterization of the interaction between these short peptides and the scaffold material is crucial to assess the potential of such a combined system. In this work, the interaction between poly(ethyl acrylate) (PEA) and 90/10 ethyl acrylate-acrylic acid copolymer P(EAcoAAc) with the SAP RAD16-I has been followed using a bidimensional simplified model. By means of the techniques of choice (congo red staining, atomic force microscopy (AFM), and contact angle measurements) the interaction and self-assembly of the peptide has proven to be very sensitive to the wettability and electronegativity of the polymeric substrate.

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Section: On the Acrylates Interaction With Rad16-isupporting

confidence: 55%

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

et al. 2016

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“…Las propiedades físicas que caracterizan al PEA son la flexibilidad de la cadena principal, la naturaleza polar y la longitud de las cadenas laterales (19) . En cuanto a las características relacionadas con su comportamiento en entornos biológicos, se trata de un polímero altamente compatible con distintos tipos de células in vitro, siendo unas de ellas las células neuronales (20)(21)(22)(23) . Esta alta compatibilidad biológica parece que es debida a que el PEA favorece la fibrilogénesis de laminina y fibronectina en sus superficies (24)(25)(26) , lo cual permite una correcta deposición de la matriz extracelular, la cual es reconocida por las células y se adhieren a esta con facilidad.…”

Section: Estrategias Actuales De Regeneración Neural: Uso De "Scaffolunclassified

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Gamboa-MartÍnez

Ribelles

Ferrer

2011

Journal of Bioactive and Compatible Polymers

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A hybrid scaffold was obtained by the deposition of a thin network of submicron fibrin fibrils on the microporous walls of a macroporous poly(L-lactide) (PLLA) three-dimensional structure. The fibrin coating is homogeneous across the entire substrate and allowed the pore structure remain open in the hybrid scaffold. The elastic modulus of the hybrid scaffold (0.65 MPa) was increased up to twofold compared to the pure PLLA scaffold (0.29 MPa). Mouse pre-osteoblastic cells, MC3T3, were seeded on both pure PLLA and hybrid scaffolds, and cultured for 3, 6, and 24 h. The coating enhanced the cell colonization and proliferation and provided a more homogeneous distribution of cells within the scaffolds. In addition, the coating improved the scaffold adhesion properties by supplying new binding sites to the cells that modify the transmembrane receptors involved in initial cell adhesion mechanism. The expression of the β3 integrin was observed in cells cultured on fibrin-coated scaffolds instead of the α5 integrin, which was expressed in the uncoated scaffold. These hybrid PLLA/fibrin scaffolds have cell culture features suitable to promote early tissue regeneration.

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“…Normally, the percentages of round platelets and the total number of adhering platelets are related to the degree of hydrophiticity: the more hydrophilic the surface is, the lower the number of platelets adhering to it and the smaller are the changes in platelet shape. The improved hydrophilicity causes a less tendency of absorption in the protein, blood platelet, and blood clotting factors and resulting improvement in the blood compatibility of implant material [47,48]. …”

Section: Effect Of Ha On the Corrosion Resistance Of The Coatingsmentioning

confidence: 99%

Bio-Corrosion Behavior of Ceramic Coatings Containing Hydroxyapatite on Mg-Zn-Ca Magnesium Alloy

Ding

Wang

et al. 2018

Applied Sciences

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Ceramic coatings containing hydroxyapatite (HA) were fabricated on a biodegradable Mg 66 Zn 29 Ca 5 magnesium alloy through micro-arc oxidation by adding HA particles into the electrolytes. The phase composition and surface morphology of the coatings were characterized by X-ray diffraction and scanning electron microscopy analyses, respectively. Electrochemical experiments and immersion tests were performed in Hank's solution at 37 • C to measure the corrosion resistance of the coatings. Blood compatibility was evaluated by in vitro blood platelet adhesion tests and static water contact angle measurement. The results show that the typical ceramic coatings with a porous structure were prepared on the magnesium alloy surface with the main phases of MgO and MgSiO 3 and a small amount of Mg 3 (PO 4 ) 2 and HA. The optimal surface morphology appeared at HA concentration of 0.4 g/L. The electrochemical experiments and immersion tests reveal a significant improvement in the corrosion resistance of the ceramic coatings containing HA compared with the coatings without HA or bare Mg 66 Zn 29 Ca 5 magnesium alloy. The static water contact angle of the HA-containing ceramic coatings is 18.7 • , which is lower than that of the coatings without HA (40.1 • ). The in vitro blood platelet adhesion tests indicate that the HA-containing ceramic coatings possess improved blood compatibility compared with the coatings without HA. Utilizing HA-containing ceramic coatings may be an effective way to improve the surface biocompatibility and corrosion resistance of magnesium alloys.

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Influence of the substrate's hydrophilicity on the in vitro Schwann cells viability

Cited by 50 publications

References 43 publications

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Bio-Corrosion Behavior of Ceramic Coatings Containing Hydroxyapatite on Mg-Zn-Ca Magnesium Alloy

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