Influence of the Subsurface Composition of a Material on the Adhesion of Staphylococci

Loskill, Peter; Hähl, Hendrik; Thewes, Nicolas; Kreis, Christian Titus; Bischoff, Markus; Herrmann, Mathias; Jacobs, Karin

doi:10.1021/la3004323

Cited by 35 publications

(59 citation statements)

References 47 publications

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“…Recently, a similar influence was detected on the adsorption kinetics of proteins [11][12][13] and the adhesion of bacteria [14]. These experiments used materials whose contribution to the potential had been tuned by means of surface stratification.…”

Section: Introductionmentioning

confidence: 66%

Macroscale adhesion of gecko setae reflects nanoscale differences in subsurface composition

Loskill

Puthoff

Wilkinson

et al. 2013

J. R. Soc. Interface.

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Surface energies are commonly used to determine the adhesion forces between materials. However, the component of surface energy derived from long-range forces, such as van der Waals forces, depends on the material's structure below the outermost atomic layers. Previous theoretical results and indirect experimental evidence suggest that the van der Waals energies of subsurface layers will influence interfacial adhesion forces. We discovered that nanometre-scale differences in the oxide layer thickness of silicon wafers result in significant macroscale differences in the adhesion of isolated gecko setal arrays. Si/SiO 2 bilayer materials exhibited stronger adhesion when the SiO 2 layer is thin (approx. 2 nm). To further explore how layered materials influence adhesion, we functionalized similar substrates with an octadecyltrichlorosilane monolayer and again identified a significant influence of the SiO 2 layer thickness on adhesion. Our theoretical calculations describe how variation in the SiO 2 layer thickness produces differences in the van der Waals interaction potential, and these differences are reflected in the adhesion mechanics. Setal arrays used as tribological probes provide the first empirical evidence that the 'subsurface energy' of inhomogeneous materials influences the macroscopic surface forces.

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Section: Introductionmentioning

confidence: 66%

Macroscale adhesion of gecko setae reflects nanoscale differences in subsurface composition

Loskill

Puthoff

Wilkinson

et al. 2013

J. R. Soc. Interface.

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“…44 Recent studies discovered that the influence of the subsurface material on adhesion is sensed by larger biological objects, too: The unspecific adhesion of bacteria from the Staphylococcus genus is affected by the properties of the subsurface material, as could be shown by AFM force spectroscopy measurements. 45 Moreover, adhesion experiments with setal arrays of live geckos revealed that the adhesion force was significantly varied by a change in the substrates subsurface composition. 46 …”

Section: Interactions In Biological Systemsmentioning

confidence: 99%

Is adhesion superficial? Silicon wafers as a model system to study van der Waals interactions

Loskill¹,

Hähl²,

Faidt³

et al. 2012

Advances in Colloid and Interface Science

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Adhesion is a key issue for researchers of various fields, it is therefore of uppermost importance to understand the parameters that are involved. Commonly, only surface parameters are employed to determine the adhesive forces between materials. Yet, van der Waals forces act not only between atoms in the vicinity of the surface, but also between atoms in the bulk material. In this review, we describe the principles of van der Waals interactions and outline experimental and theoretical studies investigating the influence of the subsurface material on adhesion. In addition, we present a collection of data indicating that silicon wafers with native oxide layers are a good model substrate to study van der Waals interactions with coated materials.

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“…In general, the adhesion of bacteria to a surface is determined by the nature of the bacterium, the surrounding medium, the surface chemistry, and the material composition reflecting the influence of the main interacting forces [12–13]: van der Waals forces, hydrophobic interaction and electrostatic forces. In addition, specific interactions amplify bacterial adhesion whenever corresponding binding partners are available.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic interaction governs unspecific adhesion of staphylococci: a single cell force spectroscopy study

Thewes¹,

Loskill²,

Jung³

et al. 2014

Beilstein J. Nanotechnol.

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SummaryUnspecific adhesion of bacteria is usually the first step in the formation of biofilms on abiotic surfaces, yet it is unclear up to now which forces are governing this process. Alongside long-ranged van der Waals and electrostatic forces, short-ranged hydrophobic interaction plays an important role. To characterize the forces involved during approach and retraction of an individual bacterium to and from a surface, single cell force spectroscopy is applied: A single cell of the apathogenic species Staphylococcus carnosus isolate TM300 is used as bacterial probe. With the exact same bacterium, hydrophobic and hydrophilic surfaces can be probed and compared. We find that as far as 50 nm from the surface, attractive forces can already be recorded, an indication of the involvement of long-ranged forces. Yet, comparing the surfaces of different surface energy, our results corroborate the model that large, bacterial cell wall proteins are responsible for adhesion, and that their interplay with the short-ranged hydrophobic interaction of the involved surfaces is mainly responsible for adhesion. The ostensibly long range of the attraction is a result of the large size of the cell wall proteins, searching for contact via hydrophobic interaction. The model also explains the strong (weak) adhesion of S. carnosus to hydrophobic (hydrophilic) surfaces.

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Influence of the Subsurface Composition of a Material on the Adhesion of Staphylococci

Cited by 35 publications

References 47 publications

Macroscale adhesion of gecko setae reflects nanoscale differences in subsurface composition

Macroscale adhesion of gecko setae reflects nanoscale differences in subsurface composition

Is adhesion superficial? Silicon wafers as a model system to study van der Waals interactions

Hydrophobic interaction governs unspecific adhesion of staphylococci: a single cell force spectroscopy study

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