Influence of the Thiol Position on the Attachment and Subsequent Hybridization of Thiolated DNA on Gold Surfaces

Wirtz, René; Wälti, Christoph; Tosch, P.; Pepper, M.; Davies, A. G.; Germishuizen, W. A.; Middelberg, Anton P. J.

doi:10.1021/la036027o

Cited by 17 publications

(16 citation statements)

References 22 publications

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“…The complementary specificity of DNA by the hybridization of specifically designed linker strands provides a precise means for programming the assembly of colloidal nanoparticles [14,[16][17][18]. The most studied template DNA molecules are terminal-modified DNA, in which the thiol group is modified at the 5 or 3 terminus of DNA [12,[16][17][18][19]. While self-assembly depends on interactions, sizes, and shapes [5,20], large assemblies are often constructed by using mutually complementary oligonucleotides attached to nanoparticles [16,18,19].…”

Section: Introductionmentioning

confidence: 99%

“…The most studied template DNA molecules are terminal-modified DNA, in which the thiol group is modified at the 5 or 3 terminus of DNA [12,[16][17][18][19]. While self-assembly depends on interactions, sizes, and shapes [5,20], large assemblies are often constructed by using mutually complementary oligonucleotides attached to nanoparticles [16,18,19]. Beyond the fundamental motivation to understand the underlying mechanisms involved in self-organization processes, self-assembled architectures of metallic nanoparticles have potential applicabilities in nanotechnologies [21].…”

Section: Introductionmentioning

confidence: 99%

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Preferentially linear connection of gold nanoparticles in derivatization with phosphorothioate oligonucleotides

Kim¹,

Lee²,

Kim³

et al. 2008

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preferentially linear connection of gold nanoparticles in derivatization with phosphorothioate oligonucleotides

Kim¹,

Lee²,

Kim³

et al. 2008

Journal of Colloid and Interface Science

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“…Immobilization of DNA on solid support leads to functional diagnostic materials which can be used for detection and studies of autoimmune antibody-DNA complexes in various assays [30][31][32][33][34][35]. Thus, using a series of monoclonal anti-dsDNA antibodies and synthetic DNA strands [23], Buhl and co-workers developed an optical biosensor as a possible new standard for laboratory tests [36].…”

Section: Synthetic Oligonucleotides In Indirect Sensing Of Autoantibomentioning

confidence: 99%

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

Astakhova¹

2014

J Clin Cell Immunol

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In this review, applications of synthetic oligonucleotide probes in diagnostics and studies of autoimmune antibodies against double-stranded DNA (anti-dsDNA) are described. As outlined herein, synthetic oligonucleotides and monoclonal antibodies provide appealing opportunities to develop standard simple assays for detection of anti-dsDNAs. Recent examples of anti-dsDNA detection using synthetic nucleic acid antigens include those applying surface plasmon resonance (SPR), antibody microarrays and homogeneous, or all-in-solution, detection with fluorescently-labelled probes. Since sequences of the applied nucleic acid antigens and monoclonal antibodies are known, the major benefit of these assays is the ability for the first time to clearly define the structural factors that govern the formation and stability of anti-dsDNA complexes. This is an essential first step toward understanding immune-complex mediated tissue injury in autoimmune conditions such as systemic lupus erythematosus (SLE) and arthritis, which involve production of pathogenic anti-dsDNAs. Moreover, synthetic nucleic acids and monoclonal antibodies have been demonstrated as key tools for the development of novel nucleic acid sensors for serotyping, along with studies of specificity and avidity of the antibody-dsDNA binding process. Among other antigens, fluorescent oligonucleotides prepared by click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides are proved to be very promising tools for efficient homogeneous detection of anti-dsDNAs.

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“…In an attempt to bring the anchor molecule closer to the surface while still maintaining the self-assembly properties of the DNA oligonucleotides, we investigated three other thiol configurations as well. 74 Figure 11 shows the chemical structures of the four different thiol arrangements. In addition to the sixcarbon-linker chain, a similar arrangement but with only three carbons (C3) was used.…”

Section: Functionalized Surfacesmentioning

confidence: 99%

Molecular Self-Assembly: A Toolkit for Engineering at the Nanometer Scale

Wälti¹

2007

Royal Society Series on Advances in Science

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Among the many challenges facing the development of a molecular-based nanotechnology, the directed assembly of discrete molecular objects, and their controlled integration into macroscopic structures, is fundamental. The selective self-assembly or self-organizing characteristic inherent to certain molecules, for example DNA, is a property that could be exploited to address these challenges. This integration problem can be separated into more fundamental tasks: attaching molecular anchors to the macroscopic structures with high spatial resolution; assembling the discrete molecular objects; and positioning and attaching the molecular objects onto the macroscopic structures.Here, several aspects of these tasks will be discussed, for example using short DNA molecules as molecular anchors and their attachment to electrodes separated by a few ten nanometers; the generation of branched DNA complexes by molecular self-organization; and using AC electric fields for the manipulation, orientation, and positioning of DNA molecules.

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Influence of the Thiol Position on the Attachment and Subsequent Hybridization of Thiolated DNA on Gold Surfaces

Cited by 17 publications

References 22 publications

Preferentially linear connection of gold nanoparticles in derivatization with phosphorothioate oligonucleotides

Preferentially linear connection of gold nanoparticles in derivatization with phosphorothioate oligonucleotides

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

Molecular Self-Assembly: A Toolkit for Engineering at the Nanometer Scale

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