Influence of the timing of blastomere isolation after the removal of nocodazole in bovine nuclear transfer

Tanaka, Hozumi

doi:10.1016/s0093-691x(99)00067-9

Theriogenology

1999

DOI: 10.1016/s0093-691x(99)00067-9

|View full text |Cite

Influence of the timing of blastomere isolation after the removal of nocodazole in bovine nuclear transfer

Hozumi Tanaka

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2004

2014

Publication Types

Select...

Article4

Relationship

Self Cite0

Independent4

Authors

Journals

Cited by 4 publications

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the development of NT embryos is improved by transplanting the embryonic cells immediately after cell division to the activated recipient oocytes [13,14]. Thus, the cell cycle and cell division status of blastomeres as donor cells for NT may have effects on the development of the resultant NT embryos.…”

mentioning

confidence: 99%

Improvement of the Developmental Ability of Nuclear Transfer Embryos by Using Blastomeres from In Vitro Fertilized Embryos Selected According to the Early Developmental Stage and Cell Division Status as Donor Cells in Cattle

Goto

Matoba

Imai

et al. 2011

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. This study was conducted to improve the developmental ability of nuclear transfer (NT) embryos by using blastomeres from in vitro fertilized (IVF) embryos with high quality as donor cells. The IVF embryos selected at the 2-cell stage at 24-h postinsemination (hpi) and again at the ≥8-cell stage at 48 hpi (Selected-IVF-embryos) showed the highest blastocyst formation rate among embryos. When blastomeres from the Selected-IVF-embryos (Selected-NT group) or Nonselected-IVF-embryos (Non-selected-NT group) were used as donor cells for NT, the blastocyst formation rate in the Selected-NT group (25.6%) was significantly higher than that in the Non-selected-NT group (13.5%). When blastomeres from the Selected-IVF-embryos at 108 (contained many cells before cell division) and 126 hpi (contained many cells immediately after cell division) were used as donor cells for NT (108-and 126-NT groups, respectively), the 126-NT group showed a significantly higher blastocyst formation rate (32.1%) than the 108-NT group (16.8%). Embryo transfer of blastocysts in the 126-NT group showed that 11 of 23 recipients became pregnant; nine calves were obtained. For the NT embryos reconstructed using in vivo derived embryos, 9 of 20 recipients became pregnant; seven calves were obtained. These results indicate that the blastocyst formation rate of NT embryos can be improved by using blastomeres from IVF embryos selected at the early developmental stage, especially immediately after cell division, and that the resultant NT embryos have a high developmental ability to progress to term that is comparable to NT embryos reconstructed using in vivo derived embryos. Key words: Bovine, Embryo, In vitro fertilization, Nuclear transfer (J. Reprod. Dev. 57: [249][250][251][252][253][254][255] 2011) uclear transfer (NT) technologies are now widely used by numerous laboratories to produce clone animals from embryonic and somatic cells. However, the overall efficiency of clone animal production is influenced by the different sources of donor cells; early pregnancy losses of cattle established from somatic nuclear transfer (sNT) are commonly above 50% [1,2], and the frequency of early fetal mortality is about two times higher with sNT than with embryonic nuclear transfer [3]. Therefore, it can be expected that a higher proportion of transferred NT embryos reconstructed from less-differentiated embryonic blastomeres would result in viable offspring compared with sNT. In addition, the production of embryonic cloned calves is permitted in Japan and food products derived from NT cattle can be on the market, thereby increasing commercial acceptance of the technology. Furthermore, the use of blastomeres from the same embryos for nuclear transfer to produce numerous identical bovine offspring is of great value for multiplication of genotypes having a superior economic value and is expected to contribute to their genetic improvement.In NT technology using embryos at an early developmental stage as donor cells, in vitro fertilized (IVF) embryos are oft...

show abstract

mentioning

confidence: 99%

Improvement of the Developmental Ability of Nuclear Transfer Embryos by Using Blastomeres from In Vitro Fertilized Embryos Selected According to the Early Developmental Stage and Cell Division Status as Donor Cells in Cattle

Goto

Matoba

Imai

et al. 2011

Journal of Reproduction and Development

View full text Add to dashboard Cite

show abstract

Developmental Potential of Bovine Androgenetic and Parthenogenetic Embryos: A Comparative Study1

Lagutina

Lazzari

Duchi

et al. 2004

Biology of Reproduction

View full text Add to dashboard Cite

In this study, we compared the developmental capacity of bovine haploid and diploid androgenetic and parthenogenetic embryos obtained by different methods. Androgenetic embryos were produced by piezo-intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) of enucleated oocytes with or without subsequent pronuclear transfer from one haploid zygote to another. Parthenogenetic embryos were obtained by activation of matured oocytes by ionomycin combined with cycloheximide or 6-dimethylaminopurine (DMAP) treatment. Only few cleaved androgenetic haploid embryos were able to compact (2.7%) and to form blastocysts (1.8%), while significantly more haploid parthenogenotes underwent compaction (24-37%) and a minority developed to blastocysts at different rates, depending on the activation procedure (cycloheximide 3%, 6-DMAP 14.5%). By contrast, development to blastocyst of diploid androgenotes, cloned androgenetic embryos, and parthenogenotes (31%, 39%, and 43%, respectively) was similar to IVF control embryos (35%). Cell number on Day 7 was higher for IVF blastocysts and decreased in consecutive order in diploid androgenotes, diploid parthenogenotes, and haploid uniparental embryos. Following transfer of diploid androgenetic embryos, a pregnancy was established and maintained up to Day 28.

show abstract

Full-Term Development of Rat after Transfer of Nuclei from Two-Cell Stage Embryos1

Popova

Bäder

Krivokharchenko

2006

Biology of Reproduction

View full text Add to dashboard Cite

Cloning technology would allow targeted genetic alterations in the rat, a species which is yet unaccessible for such studies due to the lack of germline-competent embryonic stem cells. The present study was performed to examine the developmental ability of reconstructed rat embryos after transfer of nuclei from early preimplantation stages. We observed that single blastomeres from two-cell embryos and zygotes reconstructed by pronuclei exchange can develop in vitro until morula/blastocyst stage. When karyoplasts from blastomeres were used for the reconstruction of embryos, highest in vitro cleavage rates were obtained with nuclei in an early phase of the cell cycle transferred into enucleated preactivated oocytes or zygotes. However, further in vitro development of reconstructed embryos produced from blastomere nuclei was arrested at early cleavage stages under all conditions tested in this study. In contrast, immediate transfer to foster mothers of reconstructed embryos with nuclei from two-cell embryos at an early stage of the cell cycle in preactivated enucleated oocytes resulted in live newborn rats, with a general efficiency of 0.4%-2.2%. The genetic origin of the cloned offspring was verified by using donor nuclei from embryos of Black Hooded Wistar rats and transgenic rats carrying an ubiquitously expressed green fluorescent protein transgene. Thus, we report for the first time the production of live cloned rats using nuclei from two-cell embryos.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Influence of the timing of blastomere isolation after the removal of nocodazole in bovine nuclear transfer

Cited by 4 publications

References 33 publications

Improvement of the Developmental Ability of Nuclear Transfer Embryos by Using Blastomeres from In Vitro Fertilized Embryos Selected According to the Early Developmental Stage and Cell Division Status as Donor Cells in Cattle

Improvement of the Developmental Ability of Nuclear Transfer Embryos by Using Blastomeres from In Vitro Fertilized Embryos Selected According to the Early Developmental Stage and Cell Division Status as Donor Cells in Cattle

Developmental Potential of Bovine Androgenetic and Parthenogenetic Embryos: A Comparative Study1

Full-Term Development of Rat after Transfer of Nuclei from Two-Cell Stage Embryos1

Contact Info

Product

Resources

About