Gears are one of the most crucial parts of power transmission systems in various industrial applications. Recently, there emerged a need to design gear drivers due to the rising performance requirements of various power transmission applications, such as higher load-carrying capacity, higher strength, longer working life, lower cost, and higher velocity. Due to their excellent properties, gears with asymmetric teeth have been designed to obtain better performance in applications. As the rotation speed of the gear transmission increases, the dynamic behavior of the gears has become a subject of growing interest. The most important contributing factor of dynamic behavior is the stiffness of the teeth, which changes constantly throughout the operation. The calculation of gear stiffness is important for determining the load distribution between the gear teeth when two sets of teeth are in contact. The primary objective of this article is to develop a new approach to calculate gear mesh stiffness for asymmetric gears. With this aim in mind, single tooth stiffness was calculated in the first stage of the study using a finite element method. This study presents crucial results to gear researchers for understanding spur gears with involute asymmetric teeth, and the results will provide researchers with input data for dynamic analysis.