Influence of trace elements on enzyme production: protopectinase expression by a Geotrichum klebahnii strain

Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain

“…The composition of DRM, including the vitamin solution, was similar to that already reported [6], namely (per liter): 5 g glucose (limiting substrate), 1 g K 2 HPO 4 , 0.1 g CaCl 2 Á2H 2 O, 0.6 g MgSO 4 Á7H 2 O, 0.6 g urea, 0.45 g citric acid, 1 mL vitamin solution, 1 mL trace element solution C, and 1 mL trace element solution A, pH 5.0. The concentration of trace elements solutions used in this work correspond to 1:70 of the originally (RM) reported by Cavalitto et al [5].…”

“…Typical values of enzyme activity attained using the synthetic medium were about 10-11 U/mL. Working with the same medium and microorganism, it was found that the trace elements (Mo, I, B, Co, Mn, Fe, Cu, Zn) have a critical role on PPase-SE production [6]. When the concentration of all trace elements was diminished to 1:70 or 1:80 of their original value, the concentration of PPase-SE increased around nine-fold in comparison with the first reported synthetic medium.…”

Purification and characterization of a novel alkaline α-L-rhamnosidase produced by Acrostalagmus luteo albus

“…Therefore, the development of new commercially viable processes is desirable for the bulk production of this enzyme by fermentation. Fungal enzyme expression can be increased up to tenfold by optimizing the fermentation conditions [3,8]. Thus, the cloning of the corresponding gene in order to overproduce this enzyme is now in progress.…”

Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin

“…PPase-SE is highly efficient for the enzymatic extraction of pectin from citrus peel (Sakai and Okushima, 1980) and for maceration of potato tissues to obtain single-cell foods (Nakamura et al 1995). Effects of culture conditions and medium composition on PPase-SE production have been published previously (Cavalitto et al 2000;Ferreyra et al 2002;Cavalitto and Mignone, 2007). This strain assimilates and grows on different monosaccharides (glucose, fructose, galactose and xylose) used as carbon and energy source (CES), yielding high levels of PPase-SE.…”

“…PGase activity of PPase-SE was determined as previously reported (Ferreyra et al 2002). Growth in Erlenmeyers cultures using different pectin related substances was determined by microscopic observations.…”