Influence of traditional Chinese medicines on the in vivo metabolism of lopinavir/ritonavir based on UHPLC-MS/MS analysis

Li, Linlin; Xin-Xiang, YU; Xie, Dongmin; Peng, Ningning; Wang, Weilin; Wang, Decai; Li, Binglong

doi:10.1016/j.jpha.2021.06.006

Cited by 10 publications

(7 citation statements)

References 33 publications

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“…In addition, LHQW is often clinically used in combination with other anti-viral drugs for the treatment of COVID-19. For example, LHQW in combination with ribavirin [26] , lopinavir/ritonavir [27] , and umifenovir [28] can be the first choice of the treatment for patients with severe COVID-19 patients. When combined with macrolide antibiotics, Huoxiang Zhengqi dropping pills, treat nausea, vomiting, and limb soreness [26] , not only decrease the use of antibiotics but also reduce the progression of COVID-19 cases to severe disease [26] .…”

Section: Clinical Studies Of Tmtfs In the Treatment Of Mild-tomoderat...mentioning

confidence: 99%

“Three medicines and three formulas” in COVID-19: From bench to bedside

Lyu,

Xiao,

Wang

et al. 2023

Acupuncture and Herbal Medicine

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Chinese guideline has been proven effective in the fight against Coronavirus disease 2019 (COVID-19) during the epidemic spread globally. Traditional Chinese medicine (TCM) has been widely recognized for its effectiveness in alleviating symptoms, inhibiting disease deterioration, reducing mortality, and improving cure rate of COVID-19 patients. During the pandemic, “three medicines and three formulas” stood out from hundreds of registered clinical studies and became the highly recommended TCM for COVID-19 treatment. The “three medicines and three formulas” not only effectively relieve the clinical symptoms of fever, cough, fatigue, and phlegm, but also significantly shorten the time of nucleic acid negative conversion, improve lung computed tomography imaging feature and inflammation, ameliorate clinical biochemical indicators, and reduce sequelae. The potential pharmacological mechanisms of them are mainly relevant with the crosstalk of viral toxicity, endothelial damage, cytokine storm, immune response, and microthrombus. In brief, the clinical effects as well as the potential mechanisms of “three medicines and three formulas” on COVID-19 were systematically analyzed and summarized covering the whole stages of disease development, including virus invasion and replication, immune response and cytokine storm, and acute respiratory distress syndrome and multiple organ dysfunction syndrome. We hope that this review could provide theoretical basis and reference for in-depth understanding the positive role of “three medicines and three formulas” for COVID-19 treatment.

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Section: Clinical Studies Of Tmtfs In the Treatment Of Mild-tomoderat...mentioning

confidence: 99%

“Three medicines and three formulas” in COVID-19: From bench to bedside

Lyu,

Xiao,

Wang

et al. 2023

Acupuncture and Herbal Medicine

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“…Functional herbs, used in food supplements or other healthcare products, comprise intricate chemical systems that are generally composed of multiple categories of primary and secondary plant metabolites. − These components exhibit a wide range of molecular weight, acid–base properties, polarity, etc., which poses significant difficulties in achieving the systematic characterization of the contained multiclass metabolites. − The preliminary identification of numerous secondary metabolites in herbs holds significance in providing vital clues in accelerating the discovery of potentially bioactive compounds for developing food supplements or performing quality control. Among the versatile modern analytical techniques, liquid chromatography–mass spectrometry (LC–MS) has emerged as one of the preferred tools enabling rapid multicomponent characterization due to its characteristics of excellent selectivity, remarkable sensitivity, and extensive versatility. − However, most of the commonly used LC–MS strategies are based on one-dimensional chromatography, which may suffer from insufficient peak capacity and poor resolution because of the single mechanism of separation. , In this context, more powerful separation strategies should be developed, such as multidimensional chromatography (MDC).…”

Section: Introductionmentioning

confidence: 99%

Two Multidimensional Chromatography/High-Resolution Mass Spectrometry Approaches Enabling the In-Depth Metabolite Characterization Simultaneously from Three Glycyrrhiza Species: Method Development, Comparison, and Integration

Liu,

Zhao,

et al. 2024

J. Agric. Food Chem.

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Two offline multidimensional chromatography/high-resolution mass spectrometry systems (method 1: fractionation and online two-dimensional liquid chromatography, 2D-LC; method 2: fractionation and offline 2D-LC) were established to characterize the metabolites simultaneously from three Glycyrrhiza species. Ion exchange chromatography in the first-dimensional ( 1 D) separation was well fractionated between the acidic (mainly triterpenoids) and weakly acidic components (flavonoids). These obtained subsamples got sophisticated separation by the second ( 2 D) and third dimension ( 3 D) of chromatography either by online reversed-phase chromatography × reversed-phase chromatography (RPC × RPC) or offline hydrophilic interaction chromatography × RPC (HILIC × RPC). Orthogonality for the 2 D/ 3 D separations reached 0.73 for method 1 and 0.81 for method 2, respectively. We could characterize 1097 compounds from three Glycyrrhiza species based on an in-house library and 33 reference standards, involving 618 by method 1 and 668 by method 2, respectively. They exhibited a differentiated performance and complementarity in identifying the multiple subclasses of Glycyrrhiza components.

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“…High-resolution mass spectrometry (HRMS), such as quadrupole time-of-flight mass spectrometry (Q-TOF-MS), provides an excellent tool for comprehensive chemical profiling of Chinese herbal medicines. [9][10][11] It has been demonstrated that quick chemical identification and characterization can be achieved based on HRMS and MS n data through comparisons with reference standards and reference documents. 12,13 Given that Chinese medicines have multiple components and targets, 7,14 it is of significance that quality control relies on more advanced methods or efficient strategies for evaluation of the integral components.…”

Section: Introductionmentioning

confidence: 99%

“…High‐resolution mass spectrometry (HRMS), such as quadrupole time‐of‐flight mass spectrometry (Q‐TOF‐MS), provides an excellent tool for comprehensive chemical profiling of Chinese herbal medicines 9–11 . It has been demonstrated that quick chemical identification and characterization can be achieved based on HRMS and MS n data through comparisons with reference standards and reference documents 12,13 .…”

Section: Introductionmentioning

confidence: 99%

Chemical profiling and quality evaluation of Liuweizhiji Gegen‐Sangshen oral liquid by UPLC‐Q‐TOF‐MS and HPLC‐diode array detector fingerprinting

Wei,

Jiang,

et al. 2024

Phytochemical Analysis

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IntroductionLiuweizhiji Gegen‐Sangshen (LGS) oral liquid is a Chinese patent medicine that is widely used for the prevention and treatment of alcoholic liver disease in clinical practice. However, the chemical complexity of LGS has not yet been investigated.ObjectiveThe aim of this study was to rapidly identify chemical constituents of LGS and establish a quality control method based on fingerprint and quantitative analysis.MethodologyA comprehensive strategy was used by combining qualitative analysis by ultra‐performance liquid chromatography tandem quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS) and fingerprint analysis by high‐performance liquid chromatography with diode array detection (HPLC‐DAD).ResultsA total of 162 chemical components in LGS, including 91 flavonoids, 31 organic acids, and 20 phenolic compounds, were identified or preliminarily characterized in both positive and negative ion modes based on the UPLC‐Q‐TOF‐MS results. Of these, 37 were confirmed with the reference standards. In fingerprint analysis, 23 peaks were chosen as common peaks and used to evaluate the similarity of different batches of LGS. Subsequently, a rapid quantification method was optimized and validated for the simultaneous determination of multiple chemical markers in LGS. The validated quantitative method was successfully used to analyze different batches of LGS samples.ConclusionThe proposed comprehensive strategy combining HPLC‐DAD fingerprinting and multi‐component quantification demonstrated satisfactory results with high efficiency, accuracy, and reliability. This can be used as a reference for the overall quality consistency evaluation of Chinese patent medicines.

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Influence of traditional Chinese medicines on the in vivo metabolism of lopinavir/ritonavir based on UHPLC-MS/MS analysis

Cited by 10 publications

References 33 publications

“Three medicines and three formulas” in COVID-19: From bench to bedside

“Three medicines and three formulas” in COVID-19: From bench to bedside

Two Multidimensional Chromatography/High-Resolution Mass Spectrometry Approaches Enabling the In-Depth Metabolite Characterization Simultaneously from Three Glycyrrhiza Species: Method Development, Comparison, and Integration

Chemical profiling and quality evaluation of Liuweizhiji Gegen‐Sangshen oral liquid by UPLC‐Q‐TOF‐MS and HPLC‐diode array detector fingerprinting

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