We sequenced the complete mitochondrial genome (mitogenome) of
Mustela sibirica
in China by the shotgun genome skimming methods. The mitogenome of
M. sibirica
is 16,558bp in length with the base composition of 32.9% A, 27.3% T, 26.0% C, and 13.9% G, and consists of 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and one non-coding control region. The 13 PCGs use ATG as initiation codons except
ND3
,
ND5
and
ND2
which initiate with codons ATA and ATT, respectively. Four (
COX3
,
ND1
,
ND2
and
ND4
) of the 13 PCGs terminate with a single T– –, and the remainder with a TAA termination codon except
ND3
and
CYT B
using TA– and AGA as termination codon. The phylogenetic tree based on 13 protein-coding genes indicated that
M. sibirica
is sister to the clade grouped with three species
M. nigripes
,
M. eversmannii
, and
M. putorius
, and Mustelinae species formed a monophyletic group, which is close to the Lutrinae clade within Mustelidae.