Influence of triamcinolone and somatostatin on morphometric parameters of cultured intestinal mucosa

Schneider, A.; Grosch, G; Stange, Eduard F.; Ditschuneit, H.

doi:10.1007/bf02623478

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…In the jejunal crypt epithelium, only a slight (nonsignificant; P Ͼ 0.05) decrease in the number of mitotic cells occurred under 3-day treatment, and other parameters remained unchanged, indicating that entry into mitosis was probably violated in the jejunal epithelial cells under this protocol, but the passage of cells through other phases of the cell cycle (G 1 -S-G 2 ) was not affected. Our data regarding the acute glucocorticoid effect on proliferation in the esophagus and small intestine are similar to those reported by Gololobova, 1 Batt and Scott, 12 Scott and Peters, 20 and Schneider et al 36 It should also be noted that previous studies also documented that acute glucocorticoid treatment prolonged all phases of the cell cycle, including blockade of G 1 -S and G 2 -M transitions. 1,14,37,38 Treatment with glucocorticoid for either 33 or 63 days produced a significant (P Ͻ 0.05) increase in all portions proliferating cells in the esophageal epithelium and in the epithelium of jejunal crypts.…”

Section: Discussionsupporting

confidence: 87%

Effect of acute and chronic glucocorticoid treatments on epithelial cell proliferation in the esophagus and small intestine of rats

Гунин

Nikolaev

1999

Journal of Gastroenterology

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Data about glucocorticoid influence on proliferation in the esophagus and small intestine are very contradictory and need to be reexamined. Moreover, only the effects of acute or short-term treatments with glucocorticoids have been demonstrated, whereas nothing is known about their effects under chronic exposure. This work was therefore carried out to examine proliferative activity in the esophagus and small intestine under conditions of acute and chronic glucocorticoid exposure. Rats were treated with either glucocorticoid triamcinolone acetonide or vehicle for 3, 33, or 63 days. Proliferation was assessed in the basal layer of esophageal epithelium and in the epithelium of jejunal crypts, using three criteria, as the number of mitotic, bromodeoxyuridine-labelled, and proliferating cell nuclear antigen-labelled cells. Treatment with glucocorticoid for 3 days led to a slight decrease in all parameters in the esophageal epithelium and had almost no effect on proliferation in the epithelium of jejunal crypts. Long-term treatment with glucocorticoid for either 33 or 63 days resulted in an increase in all parameters tested in both esophageal and jejunal crypt epithelia. Sixty-three-day treatment had a more prominent and significant (P < 0.05) effect. These results suggest that acute glucocorticoid treatment nonsignificantly reduces the number of cells in the cell cycle in the esophageal epithelium, whereas chronic treatment increases the number of proliferating cells in both esophageal and jejunal crypt epithelia.

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Section: Discussionsupporting

confidence: 87%

Effect of acute and chronic glucocorticoid treatments on epithelial cell proliferation in the esophagus and small intestine of rats

Гунин

Nikolaev

1999

Journal of Gastroenterology

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“…Previous observations also documented analogous changes in the stomach and colon [1, 2, 4, 5, 6, 7, 9, 10, 12, 14, 15, 28, 29]. It should be noted that a decrease in the values of all parameters is observed.…”

Section: Discussionmentioning

confidence: 71%

“…Glucocorticoid hormones play an important role in this regulation. There are much more than a hundred sources in the Medline database which have clearly proved that glucocorticoids have a strong oppressive influence on proliferative processes in the esophagus, stomach, different parts of the intestine and liver, and can lead to ulcer formation in the stomach and duodenum [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18]. At present, the situation with glucocorticoid action on proliferation in the digestive system is generally accepted.…”

Section: Introductionmentioning

confidence: 99%

Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of Rats

Гунин

Nikolaev²

2000

Digestion

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Background/Aims: It has been shown that acute or short-term treatments with glucocorticoids lead to a marked decrease in proliferation in the stomach and large intestine. The effects of more prolonged glucocorticoid treatment on cell renewal in these organs are not known. The present work was therefore undertaken to examine the proliferative activity in the stomach and colon during 2 months of glucocorticoid treatment in comparison with shorter treatments. Methods: Rats were treated with either the glucocorticoid triamcinolone acetonide or vehicle for 63, 33 or 3 days. Proliferation was assessed in the glandular epithelium of the fundal part of the stomach and in the epithelium of the colonic crypts using three criteria: the mitotic index; the bromodeoxyuridinelabelling index, and the proliferating cell nuclear antigen-labelling index (percentage of mitotic or labelled cells). Results: Treatment with glucocorticoid for 63 days resulted in a very significant increase in all proliferative parameters tested in the gastric mucosa and the colonic crypts. On the contrary, treatments with glucocorticoid for 3 or 33 days had a marked inhibitory influence on proliferation in these tissues. Conclusion: As opposed to treatments for 3 or 33 days, glucocorticoid treatment for 2 months leads to an increase in the number of cycling cells in the gastric and colonic mucosae.

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“…Most of the T and B lymphocytes with somatostatin receptors proliferate less when treated with ligand [29]. Somatostatin or its analogues also dramatically reduce rates of proliferation in gut mucosal epithelium as measured in rabbit ileal organ cultures [30] and in rat intestines in vivo [31,32]. Fetal cartilage and bone precursor cells also express SSTRs and display blunted growth rates after treatment with somatostatin [1].…”

Section: Discussionmentioning

confidence: 99%

Expression of somatostatin in the adult and developing mouse kidney

Bates¹,

Kegg²,

Grady³

2004

Kidney International

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Influence of triamcinolone and somatostatin on morphometric parameters of cultured intestinal mucosa

Cited by 8 publications

References 37 publications

Effect of acute and chronic glucocorticoid treatments on epithelial cell proliferation in the esophagus and small intestine of rats

Effect of acute and chronic glucocorticoid treatments on epithelial cell proliferation in the esophagus and small intestine of rats

Two-Month Glucocorticoid Treatment Increases Proliferation in the Stomach and Large Intestine of Rats

Expression of somatostatin in the adult and developing mouse kidney

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