Influence of Two Extension Procedures on the Ubiquitous Microflora and the Biological Quality of Frozen Bovine Semen

Gerard, Olivier; Visser, Imke; Jansen, HB

doi:10.1111/j.1439-0531.1995.tb00626.x

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…In this study, spermatozoa showing normal acrosome were higher in case of GTLS and SP than NC. These findings are in agreement with that of Gerard et al (1995), who reported that addition of GTLS to bovine semen extender resulted in significantly higher number of spermatozoa with intact acrosome as compared with the control (NC). Similarly, Ahmad & Foote (1985) found that acrosomal integrity was improved with addition of gentamycin as compared to SP.…”

Section: Discussionsupporting

confidence: 95%

Isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (Bubalus bubalis) bull spermatozoa

2016

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This study was designed to investigate the occurrence of bacterial species in water buffalo semen at the time of collection/processing and to assess the efficacy of some selected antibiotics (GTLS; gentamycin, tylosin and linco-spectin or SP; streptomycin and penicillin) in cryodiluent on bacterial control and quality including in vivo fertility of buffalo spermatozoa. For this purpose, four experiments were conducted. In experiment 1, a total of 11 bacterial species were isolated from buffalo ejaculates. In experiment 2, total aerobic bacterial counts at post dilution and thawing were lower (P < 0.05) in GTLS than in SP or control. The majority of the bacterial isolates from ejaculates were more susceptible to GTLS than SP. In experiment 3, sperm acrosome integrity was higher (P < 0.05) in GTLS and SP compared to control. In experiment 4, the in vivo fertility results for GTLS were higher (P < 0.05) than that for SP. In conclusion, a number of bacterial species were isolated from the bubaline semen, which requires an efficient control before its use in artificial insemination program. The GTLS combination of antibiotics may be incorporated into a freezing extender/protocol without compromising the post-thaw quality and in vivo fertility of buffalo bull spermatozoa.

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Section: Discussionsupporting

confidence: 95%