Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

Zelena, Liubov; Gretsky, Igor; Gromozova, E. N.

doi:10.2478/s11535-014-0347-5

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…cDNA was synthetized from 1 µg of RNA by using GenPak® MasterMixCore kit ("Neogene", Ukraine). Real-time PCR was performed on the qTower 2.2 thermal cycler (Analytik Jena AG, Germany) under conditions described in the study [16]. The estimation of the relative expression level of gene was conducted according to the 2 −ΔΔCt method [17].…”

Section: Methodsmentioning

confidence: 99%

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Antifungal activity and gene expression of lipopeptide antibiotics in strains of genus Bacillus

et al. 2016

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To research the antifungal activity and gene expression of lipopeptide antibiotics in strains of genus Bacillus. Methods. Deferred antagonism method, PCR, qRT-PCR, MALDI-TOF mass spectrometry. Results. It was revealed that Bacillus sp. strains C6 and Lg37s had the highest antifungal activity among the five tested strains. Based on the molecular genetic methods, it was shown that the expression of genes of lipopeptide antibiotics, related to the fengycin family, occurred in all these strains. At the same time, gene expression of cyclolipopeptide iturin was found in the Bacillus sp. strains C6 and Lg37s. It was determined that Bacillus sp. C6 strain had the highest level of expression of the fengycin operon`s genes, whereas the lowest level was observed in Bacillus sp. C10 strain. By means of MALDI-TOF mass spectrometry, the presence of fengycins in the cell-free cultural fluid of Bacillus sp. C6 strain was detected. Conclusion. The direct correlation between the level of antifungal activity and the fengycin synthetases expression has not been disclosed. A higher level of antagonism detected for two Bacillus strains is more likely associated with the expression and subsequent synthesis of fengycin and iturin. K e y w o r d s: bacteria of genus Bacillus, antifungal activity, MALDI-TOF, lipopeptide antibiotics.

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Section: Methodsmentioning

confidence: 99%

“…The estimation of the relative expression level of gene was conducted according to the 2 −ΔΔCt method [17]. The expression of the 16S rRNA gene was assessed as an endogenic control [16].…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity and gene expression of lipopeptide antibiotics in strains of genus Bacillus

et al. 2016

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“…qPCR was performed with QuantStudio™ 3 Real-Time PCR System (Applied Biosystems) under temperature conditions recommended by the manufacturer. 16S rRNA gene was considered as endogenous reference (Zelena et al, 2014).…”

Section: Detection Of Bacillibactin (Dhbc Dhbf) Fengycin (Fena) and Polyglutamic Acid (Epsk) Synthesis Genes In The Genome Of Nuchc C1 Anmentioning

confidence: 99%

Microbiological and genetic characteristics of Bacillus velezensis bacillibactinproducing strains and their effect on the sulfate-reducing bacteria biofilms on the poly(ethylene terephthalate) surface

Tkachuk

Zelena

Лукаш

et al. 2021

Self Cite

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It was evaluated the antibiofilm-forming properties of NUChC C1 and NUChC C2b isolates (from the collection of the Department of Biology of the T.H. Shevchenko National University "Chernihiv Colehium") against the sulfate-reducing bacteria biofilms on the poly(ethylene terephthalate) surface. NUChC C1 and NUChC C2b isolates were isolated by classical microbiology methods on Postgate's "B" medium and their cultural-morphological, some physiological-biochemical properties and molecular-genetic characteristics were investigated. To identify bacteria the sequencing and analysis of the 16S rRNA gene were carried out. The bacteria were identified as Bacillus velezensis. Based on PCR-ISSR analysis, it was found that the studied bacteria belong to different strains. The 16S rRNA gene sequences were submitted in GenBank as MN508954.1 (NUChC C1), MN749356.1 and MN749357.1 (NUChC C2b). In the genome of B. velezensis the presence and transcriptional activity of the genes for the synthesis of bacillibactin (dhbC, dhbF), fengycin (fenA) and polyglutamic acid (epsK) were studied. Among these only genes belonging to bacillibactin synthesis operon were detected and only they demonstrated activity. The observed mode of dhbC and dhbF genes expression during 144 hours of cultivation differed between two B. velezensis strains: gradually increasing in NUChC C1 and sharply increased after 24 hours with decreasing on 144th hour in NUChC C2b. Antagonistic properties of the studied strains of B. velezensis against sulfate-reducing bacteria Desulfovibrio oryzae NUChC SRB1 and NUChC SRB2 were not observed. Siderophore-producing strains of Bacillus velezensis inhibit the formation of bacterial biofilms on the polymeric material poly(ethylene terephthalate) during its long-term exposure (50 days) in a culture of sulfate-reducing bacteria under conditions of sufficient iron supply. Bacillibactin-producing strains prevent the development of bacterial biofilms on the poly(ethylene terephthalate) surface. This is one of the reasons for the prolongation of the process of poly(ethylene terephthalate) biodegradation in natural ecosystems.

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“…Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression was developed in other work [65]. Authors created an experimental sample of a biosensor for assessing the biological effects of non-ionizing electromagnetic radiation (2.45 GHz) of the radio frequency band.…”

Section: Criterion 3 -Bioluminescence and Color Changes Bioluminescementioning

confidence: 99%

Prospects of Using Biological Test-Systems for Evaluation of Effects of Electromagnetic Fields

Marynchenko

Nizhelska²,

Shirinyan³

et al. 2019

Innov Biosyst Bioeng

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Electromagnetic fields (EMF) can occur both naturally and due to human activity. Nowadays, through the innovative communication technologies, the new sources of artificial EMFs are widely disseminated. Therefore, one needs to study the artificial EMFs and mechanisms of their influence on biosystems. The paper deals with the issues of the influence of artificial EMFs on human health, environment and biological objects. The experimental and theoretical investigations concerning an action of EMF are analyzed. The description of principles of standardization of non-specific EMFs and protection from the influence of manmade fields is presented. Possible mechanisms of EMF and electromagnetic radiation (EMR) action on biological objects, including those due to the accumulation of biogenic magnetic nanoparticles in an organism, are discussed. The aim of the research is to review state-of-the-art methods for detecting the biological effects of non-thermal non-ionizing EMF and EMR and to determine the prospects of using biological testsystems for evaluation of effects of EMFs. Hereby, the main attention is drawn to EMF sensors based on the cultures of microorganisms. The classification of existing test-systems is proposed according to criteria: 1) vitality of cells; 2) motor activity of cells; 3) bioluminescence and color changes under the influence of EMF. The need to develop simple and reliable biological indicators for various types of EMFs, especially for ultrahigh-frequency radiation in connection with the introduction of 5G communications technology, is substantiated. The necessity of standardized test-protocols for comparison of research results is emphasized. It is shown that the description of corresponding experiments should be accompanied by the following features: 1) name of the cell culture; native or modified cells; 2) physical characteristics of EMF or EMR (frequency, power, modulation, source type); 3) exposure duration of; 4) list of parameters to be investigated; 5) research methods; 6) absolute measurements, results and the relative magnitude of an effect; 7) what does the influence depend on (temperature, age of cell culture, composition of the nutrient medium); 8) inheritance of changes in cells; 9) relaxation time of the effect.

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Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

Cited by 12 publications

References 24 publications

Antifungal activity and gene expression of lipopeptide antibiotics in strains of genus Bacillus

Antifungal activity and gene expression of lipopeptide antibiotics in strains of genus Bacillus

Microbiological and genetic characteristics of Bacillus velezensis bacillibactinproducing strains and their effect on the sulfate-reducing bacteria biofilms on the poly(ethylene terephthalate) surface

Prospects of Using Biological Test-Systems for Evaluation of Effects of Electromagnetic Fields

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