The c-axis oriented hcp-Co81Ir19 magnetic films were prepared on different seed layers (Ni, Cu, Ir, Pt, Au and No seed). It was systematically investigated that the factors of surface free energy and strain energy have impact on the orientation and defects and/or internal stress with increasing the lattice mismatches ratio. Moreover, the initial permeability and the natural resonance frequency were discussed in great detail by using the comparison between calculated values and experiment values. We have found that the almost unchanged 4πM
s
and μi
are not affected, while the changed H
c
, intrinsic K
grain and fr
are strongly dependent on a seed layer and the seed layer material. Moreover, the extracted damping constant is sensitive to the defects and/or internal stress and the orientation of grains. Therefore, the soft magnetic properties and microwave properties are adjusted and optimized by the seed layers with different material.