Influence of UV-B on expression profiles of genes involved in the development of autophagy by means of microtubules

Olenieva, V. D.; Lytvyn, Dmytro I.; Yemets, А. І.; Blume, Ya. B.

doi:10.15407/dopovidi2018.01.100

Cited by 6 publications

(7 citation statements)

References 14 publications

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“…При цьому вплив усіх досліджуваних стресових чинників мав наслідком негативну регуляцію генау hxk1. Слід зазначити, що раніше нами було показано вплив опромінення УФ-В на рівні експресії генів гексокіназ [17], і було виявлено суттєве підвищення рівнів експресії генів hxk1 та hxk3, та стабільність експресії гена hxk2 після опромінення як у дозі 41 кДж/м 2 , так і 81 кДж/м 2 . Враховуючи, що на сьогоднішній день функціональна роль гексокіназ у клітинах рослин є малодослідженою, отримані дані опосередковано свідчать про специфічність ізотипів гексокіназ для реалізації стрес-індукованої відповіді.…”

Section: результати та обговоренняunclassified

Influence of sucrose starvation, osmotic and salt stresses on expression profiles of genes involved in the development of autophagy by means of microtubules

Olenieva¹,

Lytvyn²,

Yemets³

et al. 2018

Visn. ukr. tov. genet. sel.

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Aim. The aim of this work was to investigate changes in expression profiles of key genes involved in the development of autophagy by means of microtubules under the influence of sucrose starvation, osmotic and salt stresses. Methods. Arabidopsis thaliana seeds were sown aseptically on Murashige and Skoog solid medium. Salt and osmotic stresses were simulated by seed germination and seedlings cultivation on the media containing 150 mM NaCl and 10 mM mannitol, respectively. For investigation of starvation-induced autophagy plants were germinated and grown on sucrose-free medium. Results. Changes in expression of α-tubulin and atg8 genes had clearly defined stressdependent nature. Overexpression of tua1 and atg8e under starvation; tua3 and atg8f under osmotic stress; tua3 and atg8f, atg8e during salt stress indirectly testifies interaction between the structural units of autophagosomes and microtubules. It was shown that influence of investigated abiotic stimuli results in overexpression of elp3 and hda6 genes. Small increase in expression levels of hexokinase 2 and 3 was demonstrated. Conclusions. Transcriptome analysis of key genes involved in realization of autophagy induced by sucrose starvation, osmotic and salt stresses in Arabidopsis thaliana cells was conducted. Received data indirectly testifies interaction between the structural units of autophagosomes and microtubules and enables to point α-tubulin and atg8 genes, which are specific for the realization of autophagy induced by a certain abiotic stimuli. Expression profiles of elp3/deacetylases as well as hexokinases indicate the critical role of α-tubulin acetylation for autophagic response, that is involved in the development of programmed cell death.Keywords: autophagy, sucrose starvation, osmotic stress, salt stress, transcriptome analysis, α-tubulin, atg8.

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Section: результати та обговоренняunclassified

Influence of sucrose starvation, osmotic and salt stresses on expression profiles of genes involved in the development of autophagy by means of microtubules

Olenieva¹,

Lytvyn²,

Yemets³

et al. 2018

Visn. ukr. tov. genet. sel.

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“…Для структурно-биологического моделирования взаимодействия α-тубулина и белка Atg8 были использованы продукты генов tua4 (At1g04820) и atg8a (At4g21980), поскольку ранее нами было показано повышение уровней экспрессии этих генов α-тубулина в ходе развития стресс-индуцированной аутофагии [6,7]. Структуры белков α-тубулина и Atg8a были предсказаны с помощью сервера Swiss-model protein homology modeling [10].…”

Section: материалы и методыunclassified

“…Ранее нами уже было показано, что и у растений развитие аутофагии сопровождается усиленным ацетилированием α-тубулина [5]. Также нами установлено, что при развитии аутофагии, индуцированной воздействием абиотических стрессов, происходит повышение уровней экспрессии генов elp3 и hda6, продукты которых регулируют функциональное состояние МТ путем обратимой реакции ацетилирования α-тубулина [6,7].…”

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Expression profiling of kinesins, involved in the development of autophagy in Arabidopsis thaliana, and the role of tubulin acetylation in the interaction of Atg8 protein with microtubules

Olenieva¹,

Lytvyn²,

Yemets³

et al. 2018

Fakt. eksp. evol. org.

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Aim. To investigate the interrelation between changes in the expression levels of kinesin genes that are potentially involved in the development of stress-induced autophagy in Arabidopsis thaliana by means of microtubules, and the structural biology analysis of the role of α-tubulin acetylation in the regulation of interaction of α-tubulin with Atg8. Methods. The simulation of the influence of abiotic stresses. PCR analysis of changes in expression levels of kinesin genes. The molecular dynamics simulations of α-tubulin and Atg8 complexes were performed using the GROMACS 4.5.5 program. Results. It was shown that the changes in expression levels were caused by the influence of stressful stimuli. A significant increase in the transcriptional activity of the KIN5B, KIN12B, KIN12F genes after UV-B irradiation, the KIN6, KIN7O, KIN7D, KIN12B genes under osmotic-, and KIN6, KIN12B under salt stress was detected. By means of bioinformatics it was demonstrated that α-tubulin acetylation provides an enhanced interaction of α-tubulin and Atg8 protein. Conclusions. Obtained data point out the important role of kinesins and α-tubulin acetylation in realization of microtubules’ partaking in the development of stress-induced autophagy in plants. Keywords: microtubules, α-tubulin, kinesins, Atg8 protein, stress-induced autophagy.

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“…Some responses can be seen as adaptive to microgravity conditions. Under the conditions of microgravity, the expression of genes coding for proteins responsible for cell wall metabolism, hormonal status, system polarity, cytoskeleton, oxidative stress, lipid metabolism, cell division, and PCD development is significantly modified [4,6,11,19]. One of the most interesting issues related to microgravity is the induction of autophagy, which is the organism's adaptive response to stress.…”

Section: Introductionmentioning

confidence: 99%

“…One of the most interesting issues related to microgravity is the induction of autophagy, which is the organism's adaptive response to stress. We have recently shown that the development of autophagy in plant cells can be initiated by various abiotic stresses -starvation, osmotic and salt stress, UV-B irradiation [15,19,20]. Although it is considered an important mechanism for maintaining cellular homeostasis, including different stresses, the physiological role and regulation of autophagy in plants remain insufficiently studied [14-16, 19, 20, 28].…”

Section: Introductionmentioning

confidence: 99%

Elucidation of Cellular Mechanisms of Autophagy Involvement in Plant Adaptation to Microgravity Conditions

Yemets¹,

Plokhovska²,

Shadrina³

et al. 2023

Kosm. nauka tehnol.

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It was shown that clinostating conditions induce autophagy without increasing of programmed cell death (PCD) index in the epidermal cells of the root apex of A. thaliana seedlings. After the phase of activation of autophagy, its regulatory weakening occurs, which probably indicates adaptive changes to the conditions of clinostating. The induction of autophagy correlates with an increase in the expression levels of atg8 genes, some of which (atg8e and atg8i) may be involved in the implementation of autophagy under the simulated microgravity conditions. The transcriptional activity of cytoskeleton genes involved in the implementation of stress-induced autophagy, in particular α- and β-tubulin genes, was analyzed. Joint expression of α- and β-tubulin genes and atg8 under the simulated microgravity conditions was revealed. These results illustrate the role of the cytoskeleton in the development of microgravity-induced autophagy and make it possible to identify genes specific to this type of stress. The induction of autophagy and PСD was studied under the action of gamma irradiation as a concomitant factor of space flights, as well as under the combined action of acute irradiation and clinostating. Gamma irradiation in doses equivalent to those in the spacecraft cabin (1 - 6 Gy) induced dose-dependent changes in the topology and cytogenetic state of the root apical meristem, as well as slightly inhibited of the early plant development. In the meristem, heterogeneity increased, PCD indexes, mainly proliferative death and autophagy, increased. With the combined action of gamma irradiation (2 Gy) and clinostating, the density of autophagosomes in the epidermal cell root apices of 6-day-old seedlings increased (24 hours after irradiation), and after 4 days it decreased, compared to the non-irradiated control.

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Influence of UV-B on expression profiles of genes involved in the development of autophagy by means of microtubules

Cited by 6 publications

References 14 publications

Influence of sucrose starvation, osmotic and salt stresses on expression profiles of genes involved in the development of autophagy by means of microtubules

Influence of sucrose starvation, osmotic and salt stresses on expression profiles of genes involved in the development of autophagy by means of microtubules

Expression profiling of kinesins, involved in the development of autophagy in Arabidopsis thaliana, and the role of tubulin acetylation in the interaction of Atg8 protein with microtubules

Elucidation of Cellular Mechanisms of Autophagy Involvement in Plant Adaptation to Microgravity Conditions

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