Influence of various media constituents on the growth of Cinchona ledgeriana tissue cultures and the production of alkaloids and anthraquinones therein

Abstract

Using the methods reported by De Fossard et al. (11) the influence of various media constituents on the growth and the alkaloid and anthraquinone production in Cinchona ledgeriana callus cultures was studied. Growth and indole alkaloid production (e.g. cinchonamine) was improved by higher auxin levels. The best growth was observed in the light, although many media resulted in no growth at all in the light. Anthraquinone production was highest at lower auxin levels. Quinoline alkaloid levels (e.g. quinidine) were highest in media with low auxin concentrations. Low and medium cytokinin concentration benefited the quinoline alkaloid production.From the results it was concluded that the pathways leading to the various secondary products, anthraquinones, indole alkaloids and quinoline alkaloids are, at least partly, regulated independently.

The influence of initial sucrose and nitrate concentrations on the growth of Cinchona ledgeriana cell suspension cultures and the production of alkloids and anthraquinones

“…The maximum in these spectra at ca. 500 nm was used for the calculations, and alizarin was used as a calibration standard [1].…”

“…In a recent study we have reported on the influence of various medium components on the growth of C. ledgeriana tissue cultures and the production of secondary metabolites therein [ 1 ]. In that study a cell suspension culture was plated on solid media and, subsequently, growth and secondary metabolite production were determined.…”

Influence of nutrient salts, auxins and cytokinins on the in vitro growth of Salvia greggii

“…Media containing 0.25, 0.5 and 1 times the normal MS inorganic salt concentration [4] were prepared and 80 cultures of each medium were established. A second group of experiments were designed to determine the most active auxin and cytokinin or cytokinin combination to use for shoot production since different plants respond to different hormones [2]. To evaluate auxins, indoleacetic acid (IAA), indolebutyric acid (IBA), or NAA were added at 0.5 or 5.0pM to the MS medium.…”

Identification of Subcellular, Structural, and Metabolic Changes Through NMR

“…Several elicitors are being employed in in vitro cultures of plants for the increased production or induced de novo synthesis of secondary metabolites (Sudha and Ravishankar 2003a;Karuppusamy 2009). For example, 2,4-dichlorophenoxy acetic acid (2,4-D), indole acetic acid (IAA), and naphthalene acetic acid (NAA) have increased the production of indole alkaloids in cell cultures of Cinchona ledgeriana (Harkes et al 1985). Half-strength Gamborg's B 5 medium supplemented with 5 % sucrose and 1 mg/l of each of NAA and BA gave the best values in root culture growth of Datura candida x D. aurea (Nussbaumer et al 1998), whereas full-strength B 5 medium supplemented with the same concentrations of NAA and BA gave the best results for hyoscyamine and scopolamine content.…”