Influence of verapamil on pharmacokinetics of pristimerin in rats

Gao, Xiao‐Dong; Zhang, Yongjie; Wang, Yongdong; Zhang, Yijie; Wang, Yixin; Liu, Shuya; Gao, Xiangwei

doi:10.1002/bmc.3611

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…The complexes of CD and triterpenic acids were prepared according to several reported studies with minor modifications (Gao et al, 2016;Ren et al, 2016). In general, 30% β-CD (30 g β-CD dissolved in 100 mL water) was used for forming the inclusion complexes.…”

Section: β-Cd Inclusion Complexes Of Triterpenic Acidsmentioning

confidence: 99%

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

You

Zhao

et al. 2017

Biomedical Chromatography

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Triterpenic acids are widely distributed in many fruits and are known for their medicinal benefits. The study of bioavailability has been an important task for a better understanding of the triterpenic acids. Although many methods based on fluorescence labeling for triterpenic acid determination have been established, these reported methods needed anhydrous conditions, which are not suitable for the convenient study of triterpenic acid bioavailability. Inspired by that, a versatile method, which overcomes the difficulty of the reported methods, has been first developed in this study. The novel method using 2-[12-benzo[b]acridin-5- (12H)-yl]-acetohydrazide (BAAH) as the fluorescence labeling reagent coupled with high-performance liquid chromatography with fluorescence detection was first developed for the study of triterpenic acid bioavailability. Furthermore, the labeling conditions have been optimized in order to achieve the best fluorescence labeling yield. Under the optimal conditions, the quantitative linear range of analytes was 2-1000 ng mL , and the correlation coefficients were >0.9998. The detection limits for all triterpenic acid derivatives were achieved within the range of 0.28-0.29 ng mL . The proposed method was successfully applied to the study of triterpenic acid bioavailability with excellent applicability and good reproducibility.

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Section: β-Cd Inclusion Complexes Of Triterpenic Acidsmentioning

confidence: 99%

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

You

Zhao

et al. 2017

Biomedical Chromatography

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“…2015; Gao X et al. 2016). To the best of our knowledge, there are little data available for the effects of glycyrrhizin on the pharmacokinetics of paeoniflorin.…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that the glycyrrhizin, a triterpenoid saponin that isolated from liquorice, has anti-inflammatory, hepato-protective and antitumour properties. Some research articles have indicated that the drug-drug interaction might occur when they are coadministered (Hu et al 2003;Gao QT et al 2004;Chen et al 2012;Tai et al 2014;Feng et al 2015;Gao X et al 2016). To the best of our knowledge, there are little data available for the effects of glycyrrhizin on the pharmacokinetics of paeoniflorin.…”

Section: Introductionmentioning

confidence: 99%

Effects of glycyrrhizin on the pharmacokinetics of paeoniflorin in rats and its potential mechanism

Sun

Wang

2019

Pharmaceutical Biology

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Context: Paeoniflorin is reported to possess numerous pharmacological activities. Paeoniflorin and glycyrrhizin are always used together for the treatment of disease in China clinics; however, the drug–drug interaction between glycyrrhizin and paeoniflorin is still unknown. Objective: This study investigates the effects of glycyrrhizin on the pharmacokinetics of paeoniflorin in rats. Materials and methods: The pharmacokinetics of orally administered paeoniflorin (20 mg/kg) with or without glycyrrhizin pre-treatment (at a dose of 100 mg/kg/day for 7 days) were investigated in male Sprague–Dawley rats using LC-MS/MS. Additionally, Caco-2 cell transwell model and rat liver microsome incubation experiments were also conducted to investigate its potential mechanism. Results: The results showed that when the rats were pre-treated with glycyrrhizin, the C max of paeoniflorin decreased from 59.57 ± 10.24 to 45.36 ± 8.61 ng/mL, and AUC 0-inf also decreased from 282.02 ± 35.06 to 202.29 ± 28.28 μg·h/L. The t 1/2 value of paeoniflorin decreased from 8.48 ± 2.01 to 5.88 ± 1.15 h ( p < 0.05). The Caco-2 cell transwell experiments indicated that glycyrrhizin could increase the efflux ratio of paeoniflorin from 2.71 to 3.52, and the rat liver microsome incubation experiments showed that glycyrrhizin could significantly increase its intrinsic clearance rate from 53.7 ± 4.6 to 85.6 ± 7.1 μL/min/mg protein. Conclusions: These results indicated that glycyrrhizin could affect the pharmacokinetics of paeoniflorin, and it might work through decreasing the absorption of paeoniflorin by inducing the activity of P-gp or through increasing the clearance rate in rat liver by inducing the activity of CYP450 enzyme.

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“…Therefore, in the presence of verapamil, the oral bioavailability of Pristimerin is almost twice as high (1728.1 μg h/mL when given with verapamil vs. AUC0-t of 949.2 μg h/mL when given alone) in rats [12]. For these naturally occurring compounds, knowledge of toxicity or medicinal properties is usually earlier than an accurate understanding of the target or mechanism, and there is still a lot of knowledge about Pristimerin.…”

Section: Introductionmentioning

confidence: 99%

Systematic Understanding of the Mechanism of Pristimerin via Computational Target Fishing

Z¹,

Chen²,

Yu³

et al. 2018

Preprint

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Pristimerin (PM) is a naturally occurring quinonemethide triterpenoid compound that isolated from the Celastraceae and Hippocrateaceae families. Its anticancer effects have attracted a great deal of attention, but the mechanisms of action remain obscure. In this study, we screened for the active compounds of Pristimerin using a drug-likeness approach. Potential protein targets of Pristimerin were predicted by PharmMapper and Coremine database. Candidate protein targets were then uploaded to GeneMANIA and GO pathway analysis. Finally, compound-target, target-pathway, and compound-target-pathway networks were constructed using Cytoscape 3.3. The results showed that Pristimerin had good drug ability and identified 13 putative protein targets. Network analysis revealed that these targets are associated with cancer, inflammation and other physiological processes. In summary, Pristimerin is predicted to target a variety of proteins and pathways to form a network that exerts systemic pharmacological effects.

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Influence of verapamil on pharmacokinetics of pristimerin in rats

Cited by 10 publications

References 20 publications

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Development of a facile and sensitive HPLC‐FLD method via fluorescence labeling for triterpenic acid bioavailability investigation

Effects of glycyrrhizin on the pharmacokinetics of paeoniflorin in rats and its potential mechanism

Systematic Understanding of the Mechanism of Pristimerin via Computational Target Fishing

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