Influence of viscoelastic properties of an hyaluronic acid-based hydrogel on viability of mesenchymal stem cells

Abstract

is an open access repository that collects the work of Arts et Métiers ParisTech researchers and makes it freely available over the web where possible. Abstract. BACKGROUND:The present research is involved in the framework of the biotherapy using mesenchymal stem cells (MSCs). Here, MSC encapsulation in a hydrogel based on hyaluronic acid (HA) is investigated to optimize the composition of the biomaterial. METHODS: Several formulations candidates of the hydrogel (9 in total) are postulated as a scaffold for the 3D MSC culture in order to investigate their potential to mimic the in vivo cellular environment. Rheological measurements in oscillation mode of complex modulus and complex viscosity are performed on the different hydrogels. Biological tests are carried out for the measurement of the cell viability of MSC encapsulated in the hydrogels. RESULTS: Rheological and biological findings are correlated together in order to establish relationships between the viscoelastic properties of the hydrogel and the cellular viability of MSC. CONCLUSIONS:In the light of the viaility results, the composition of the hydrogel was related to the MSC proliferation. Thus, such relations are useful tools for scientists offering them more flexibility in the design of their hydrogels while ensuring an acceptable level of MSC viability.

Hydrogel scaffolds based on blood plasma cryoprecipitate and collagen derived from various sources: Structural, mechanical and biological characteristics

“…The viscoelastic properties of hydrogels are an integral result of individual characteristics and interactions of the structural component of a material (for example, fibers or filaments), chemical (for example, ionic bonds), fluid and cells [[12], [13], [14]]. There is no doubt that the viscoelastic properties of both artificial and natural ECMs directly affect cell migration, proliferation and differentiation [[15], [16], [17]]. For example, O. Chaudhuri et al (2016) found out that cells'spreading, proliferation, and osteogenic differentiation of mesenchymal stem cells (MSCs) are all enhanced in cells cultured in gels with faster relaxation [18].…”

Hyaluronic Acid: Incorporating the Bio into the Material

“…• can change viscosity depending on modification 121,137 • can affect hydrophobicity of hydrogel 167,170 incorporate interpenetrating/semiinterpenetrating networks…”

“…137 Variation of viscoelastic properties in this way influences mesenchymal stem cell (MSC) viability. 121 More recently, Lou et al employed a dynamic hydrazone bond to cross-link HA polymers within an interpenetrating network of HA and collagen I in order to confer stress relaxation to the hydrogel. Varying the crosslinker affinity, MW of HA, and concentration of HA allowed for tuning of the relaxation time, with faster relaxation times promoting MSC spreading and focal adhesion formation.…”

“…We have demonstrated that HA-based scaffolds support viability of implanted human pluripotent stem cell-derived dopaminergic neurons and neural progenitor cells for treatment of Parkinson’s disease. , HA-based hydrogels have also been explored as scaffolds for adipose tissue, , cartilage, and bone engineering . Because HA is a major component of endogenous ECM and the mechanics of HA can be tuned through a variety of parameters, HA-based biomaterials with controllable mechanics are also used as a research platform in mechanobiology. − …”

Injectable Systems for Intra-Articular Delivery of Mesenchymal Stromal Cells for Cartilage Treatment: A Systematic Review of Preclinical and Clinical Evidence

“…HA is a non-sulfated glycosaminoglycan (GAG) in cartilaginous ECM maintaining chondrocyte functions and a component of synovial fluid responsible for its viscoelasticy [ 3 ]. In vitro studies revealed that HA modulates inflammation inhibiting matrix metalloproteinases (MMPs) [ 77 ]. HA has potential biological effects, including the enhancement of the chondrogenic effects of MSCs [ 78 ] and the promotion of synovial cell or chondrocyte migration in the presence of basic fibroblast growth factors [ 79 ].…”