Influence of visible light and room temperature on cell proliferation in preimplantation rabbit embryos

Schumacher, Armin; Fischer, Bernd

doi:10.1530/jrf.0.0840197

Cited by 53 publications

(22 citation statements)

References 39 publications

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“…The arrested metaphases deserve special attention. They are not likely to be derived from an increased mitotic activity, because the embryonic growth was almost completely inhibited during culture at room temperature (Chang 1948;Schumacher and Fischer 1988). A specific susceptibility of the spindle apparatus towards reduced temperature seems to be the most likely reason.…”

Section: Discussionmentioning

confidence: 99%

Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study

1991

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In a time course study (4-20 h) rabbit early cleavage stages (day 1 p.c.) and compacted morulae (day 3 p.c.) were exposed to visible light or room temperature (23 degrees C), respectively. An 8 h light exposure of day 1 embryos caused alterations in nuclear morphology (lobulated nuclei, loss of nucleolar differentiation), an increased electron density of the cytoplasm, and cellular fragmentation leading to a considerable degeneration of blastomeres (central clustering of organelles, loss of cell surface differentiation) after a 20 h exposure. Room temperature exposure (compacted Day 3 morulae) led to decompaction and a cleavage delay after 8 h. After 10 h, arrested metaphases occurred in all examined morulae. Even after 20 h at 23 degrees C, day 3 embryos were at the decompacted morula stage, and showed metaphase-arrested blastomeres. The general morphology of the blastomeres was unaffected at this temperature, except for vacuolated ser- and cis-side vesicles of the Golgi complex at 8, 12 and 20 h, respectively.

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Section: Discussionmentioning

confidence: 99%

Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study

1991

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“…We have selected the CF-1 mouse for this study because it blocks at the 2-cell stage in all media tested in our laboratory although Dandekar & Glass (1987) Schumacher & Fischer (1988) suggested that as little as 1 h of exposure to light is significantly harmful to Day-1 rabbit embryo development. All solutions, dishes and instruments were maintained at 37°C before use.…”

Section: Introductionmentioning

confidence: 99%

An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro

Chatot¹,

Ziomek²,

Bavister³

et al. 1989

Reproduction

1,074

635

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Summary. One-cell CF-1 \m=x\ B6SJLF1/J embryos, which usually exhibit a 2-cell block to development in vitro, have been cultured to the blastocyst stage using CZB medium and a glucose washing procedure. CZB medium is a further modification of modified BMOC-2 containing an increased lactate/pyruvate ratio of 116, 1 mM-glutamine and 0\m=.\1mM-EDTA but lacking glucose. Continuous culture of one-cell embryos in CZB medium allowed 83% of embryos to develop beyond the 2-cell stage of which 63% were morulae at 72 h of culture, but blastocysts did not develop. However, washing embryos into CZB medium containing glucose after 48 h of culture (3-4-cell stage) was sufficient to allow development to proceed, with 48% of embryos reaching the blastocyst stage by 96 h of culture. Exposure of embryos to glucose was only necessary from the 3\p=n-\4-cell stage through the early morula stage since washing back into medium CZB without glucose at 72 h of culture still promoted the development of 50% of embryos to the blastocyst stage. The presence of glucose in this medium for the first 48 h of culture (1-cell to 4-cell stage) was detrimental to embryo development. Glutamine, however, exerted a beneficial effect on embryo development from the 1-cell to the 4-cell stage although its presence was not required for development to proceed during the final 48 h of culture. Blastocysts which developed under optimum conditions contained an average of 33\m=.\7total cells. The in-vitro development of 1-cell embryos beyond the 2-cell stage in response to the removal of glucose and the addition of glutamine to the culture medium suggests that glucose may block some essential metabolic process, and that glutamine may be a preferred energy substrate during early development for these mouse embryos.

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“…Lighting during embryo manipulation may be harmful for embryo viability, because physiologic brightness during in vivo development is much darker than that during the manipulation (10)(11)(12)(13)(14). Such difference may induce stress-related biologic metabolism or activate biologic reactions that lead to embryo mortality.…”

mentioning

confidence: 99%

Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro

Gong

Lee

et al. 2007

Fertility and Sterility

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Influence of visible light and room temperature on cell proliferation in preimplantation rabbit embryos

Cited by 53 publications

References 39 publications

Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study

Effects of visible light and room temperature on the ultrastructure of preimplantation rabbit embryos: a time course study

An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro

Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro

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