Medical augmented reality has undergone great development recently. However, there is a lack of studies to compare quantitatively the different display options available. This paper compares the effects of different graphical overlay systems in a simple micromanipulation task with “soft” visual servoing. We compared positioning accuracy in a real-time visually-guided task using Micron, an active handheld tremor-canceling microsurgical instrument, using three different displays: 2D screen, 3D screen, and microscope with monocular image injection. Tested with novices and an experienced vitreoretinal surgeon, display of virtual cues in the microscope via an augmented reality injection system significantly (p < 0.05) decreased 3D error compared to the 2D and 3D monitors when confounding factors such as magnification level were normalized.