Influence of Vitamin D Metabolites on Plasma Cytokine Concentrations in Endurance Sport Athletes and on Multiantigen Stimulated Cytokine Production by Whole Blood and Peripheral Blood Mononuclear Cell Cultures

Abstract

Aim. Our aims were to determine the influence of plasma total 25-hydroxy vitamin D (25(OH)D) status on the plasma cytokine concentrations in athletes and the in vitro effects of different doses of 1, 25 dihydroxyvitamin D3 (1, 25(OH)2D3) on multiantigen stimulated cytokine production by whole blood and peripheral blood mononuclear cell (PBMC) cultures. Methods. Plasma samples from 43 athletes with high and low levels of 25(OH)D were assayed for the concentrations of cytokines. The whole blood samples and PBMCs from healthy subjects were incubated in vitro with a multi-antigen vaccine and different doses of added 1, 25(OH)2D3. The circulating cytokines and stimulated whole blood and PBMC culture production of cytokines were determined using a biochip assay. Results. The circulating interleukin-(IL-)10 and interferon-(IFN-) γ concentrations were significantly higher in the vitamin D sufficient athletes. Furthermore, the production of tumour necrosis factor-(TNF-) α, IL-6, IFN-γ, IL-2, and IL-10 by whole blood culture was significantly inhibited by 1, 25(OH)2D3 concentrations of 1000 pmol/L or 10000 pmol/L. Conclusions. We found that the influence of vitamin D on circulating cytokines might be different in athletes compared with nonathletes and cytokines production by whole blood culture was not influenced by 1, 25(OH)2D3 in concentrations within the normal healthy range.

Vitamin D status and its modulatory effect on interferon gamma and interleukin-10 production by peripheral blood mononuclear cells in culture

“…The findings of our study are consistent with what He et al [21] reported in their study. They cultured both whole blood and PBMCs in presence and absence of vitamin D but used a multi-antigen vaccine containing tetanus, Haemophilus influenzae type b, diphtheria, poliomyelitis and acellular pertussis antigens as a stimulant and assessed the use of different vitamin D concentrations in the culture of six samples.…”

The impact of sleeping with reduced glycogen stores on immunity and sleep in triathletes

“…This decrease in vitamin D was accompanied with a slight decrease in sIgA concentration ( d = 0.23) only in the SL group over weeks of training. This result is in conformity with previous studies reporting a positive correlation between the vitamin D status and the sIgA secretion (He et al 2014 ). However, it is worth noting that the decrease in vitamin D could have been also induced by the low direct exposition to UVB, since this study was conducted in the winter season at a high latitude (Paris, 53°N).…”

Supplemental vitamin D increases serum cytokines in those with initially low 25-hydroxyvitamin D: A randomized, double blind, placebo-controlled study

“…A recent study in endurance athletes demonstrated that vitamin D insufficiency associated with a decrease in IFN-c while 1,25(OH)D inhibited IFN-c production by peripheral blood mononuclear cells [39]. This paradox suggests a pro-inflammatory cytokine decrease with low 25(OH)D and following 1,25(OH)D exposure, thereby underscoring the complexity of investigating the influence of vitamin D on cytokines and extrapolating in vitro results to in vivo conditions.…”