Influence of water availability on the enzymatic hydrolysis of proteins

Butré, Claire I.; Wierenga, Peter A.; Gruppen, Harry

doi:10.1016/j.procbio.2014.08.009

Cited by 27 publications

(17 citation statements)

References 35 publications

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“…In particular, in the hydromodule 1:2, the higher substrate concentration lead to a higher release of N-species during the first hour, compared to the other ratio, but with a lower value at the end of the reaction, (%DH=17.3 ± 0.1). Conversely, the more diluted solution with hydromodule 1:3, generated more N-free species at the end of the hydrolysis reaction (%DH = 19.2 ± 0.2), consistent with the findings of Butrè et al (2014a). The latter authors demonstrated that water availability plays a crucial role in the hydrolysis of proteins (at constant enzyme/substrate ratio) by increasing protein concentration and decreasing the overall rate of enzymatic protein hydrolysis.…”

Section: Enzymatic Hydrolysis At Lab-and Medium-scalesupporting

confidence: 85%

Enzymatic Hydrolysis as a Way to Recovery Bovine Hides: Laboratory and Medium Scale Trials, Characterization of the Hydrolysates and Scale-Up to Semi-Industrial Scale

et al. 2018

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A current hot topic in the field of environmental protection and development of valuable new products is represented by the recovery of food waste from the meat sector. The present study focused on the recovery of bovine hides by means of a sustainable method. Enzymatic hydrolysis was selected as a potential green methodology for use in the production of protein hydrolysates to be applied on an industrial scale. For this purpose, the enzymatic hydrolysis of bovine hides with Alcalase was investigated following a multiscale approach: lab-scale, medium-scale and semi-industrial pilot plant. Alcalase proved to be highly efficient due to its ability to degrade collagen, the main protein of hides. Under optimized conditions, the hydrolysis of hides at laband medium-scales resulted in approx. 85% protein solubilisation after 6 hours, with a consistent release of free amino acids and a degree of hydrolysis of 17-19%. However, in the pilot plant, solubilisation decreased due to difficulties in mixing bovine hides in the reactor, which was compensated with a longer reaction time to achieve virtually total protein solubilisation (∼98%). The present data therefore demonstrate the applicability of the process at semi-industrial scale in protein recovery with a reduced amount of waste by-products.

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Section: Enzymatic Hydrolysis At Lab-and Medium-scalesupporting

confidence: 85%

Enzymatic Hydrolysis as a Way to Recovery Bovine Hides: Laboratory and Medium Scale Trials, Characterization of the Hydrolysates and Scale-Up to Semi-Industrial Scale

et al. 2018

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“…The main peak (78%) was identified to be the enzyme BLP (23.6 kDa). A second smaller peak (14% of total UV 214 area) was due to the pro-peptide (6.9 kDa) as described previously [17]. The enzyme has an activity of 3.9 AU/mg/min as determined by the azocasein assay.…”

Section: Methodsmentioning

confidence: 64%

“…The BLP (4.5% (w/w) protein, by the Dumas method N × 6.25; activity of 0.3 AU/mg/min as determined by the azocasein assay [16]) was partly insoluble and was fractionated as described before [17]. The freeze dried, water soluble material was found to contain 60% (w/w) protein (N × 6.25).…”

Section: Methodsmentioning

confidence: 99%

Spontaneous, non-enzymatic breakdown of peptides during enzymatic protein hydrolysis

Butré

Bühler

Sforza

et al. 2015

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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“…While the proteases used in this study have specificity for the breakdown of proteins, Alcalase has a preference for large residue carboxyl sites without load of proteins and peptides links to adjacent hydrophobic or aromatic amino acids, such as tyrosine (Butré, Wierenga, & Gruppen, ; Paraman, Hettiarachchy, Schaefer, & Beck, ; Rossini, Noreña, Cladera‐Olivera, & Brandelli, ). Even though the specificities of Neutrase and Protamex are not clearly defined (Dryáková, Pihlanto, Marnila, Čurda, & Korhonen, ), it is known that they are endoproteases characterized by their ability to hydrolyze internal peptides bonds (Liu et al, ), which preferentially release hydrophobic amino acids: phenylalanine, isoleucine, leucine, methionine, and valine (Dijk, Folkertsma, & Dekker, ).…”

Section: Resultsmentioning

confidence: 99%

Section: Nutritional and Bioactive Resultsmentioning

confidence: 99%

Effect of enzymatic hydrolysis on structural characteristics and bioactive composition of bee‐pollen

Zuluaga-Domínguez

Castro‐Mercado

Quicazán

2019

J Food Process Preserv

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Bee‐pollen is a product collected by bees, gathered for human consumption given its nutritional and bioactive characteristics. However, its external structure is extremely recalcitrant and prevents nutrients and bioactive compounds to be completely digested into the gastrointestinal tract. The aim of this study was to assess enzymatic hydrolysis as a strategy to modify the structure of bee‐pollen and promote the release of compounds. Six different commercial enzymes were used and treatments were evaluated by Differential Scanning Calorimetry and Scanning Electron Microscopy, as well as protein, amino acids, phenolics, flavonoids, and antioxidant activity. The calorimetric analysis showed an increase in the heat flow of hydrolyzed products compared to bee‐pollen. It was also found that proteases improved the protein content of about 13%–18%, phenolics 83%–106%, flavonoids 85%–96%, antioxidant activity up to 68%, and increased all essential amino acids. Finally, a total ranking method established as the best treatment hydrolysis with Protamex. Practical applications Bee‐pollen is an attractive food of plant origin from a nutritional and functional point of view. The modification of its structure through enzymatic hydrolysis increases the availability of compounds that theoretically could be assimilated more easily in the gastrointestinal tract, improving the quality of bee‐pollen as a food resource. The treatment is safe, relatively inexpensive and easy to apply, compared to other bee‐pollen modification strategies such as fermentation.

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Influence of water availability on the enzymatic hydrolysis of proteins

Cited by 27 publications

References 35 publications

Enzymatic Hydrolysis as a Way to Recovery Bovine Hides: Laboratory and Medium Scale Trials, Characterization of the Hydrolysates and Scale-Up to Semi-Industrial Scale

Enzymatic Hydrolysis as a Way to Recovery Bovine Hides: Laboratory and Medium Scale Trials, Characterization of the Hydrolysates and Scale-Up to Semi-Industrial Scale

Spontaneous, non-enzymatic breakdown of peptides during enzymatic protein hydrolysis

Effect of enzymatic hydrolysis on structural characteristics and bioactive composition of bee‐pollen

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