Influence of Water Polarization Caused by Phonon Resonance on Catalytic Activity of Enolase

Danielewicz, Regina; Wandzel, Czesław; Beznosiuk, Jarosław; Szuba, Andrzej; Samsel‐Czekała, M.; Gamian, Andrzej

doi:10.1021/acsomega.0c05199

Cited by 3 publications

(4 citation statements)

References 27 publications

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“…In the present Perspective, we focus on examples based on these enzymes, but note that other completely different kinds of fleeting chiral intermediates also occur in other enzyme mechanisms which likewise have not been analyzed with respect to the configuration of the reactive species. Additional examples also include short-lived chiral octahedral metal complexes, as in some enolases. − Finally, short-lived chiral tetrahedral radicals undergoing fast and reversible racemization also need attention, as in the mechanism of P450-catalyzed oxidative hydroxylation − and in other biological processes. − The proposed classification should be continually extended to include additional enzymatic reactions in which fleeting chiral intermediates occur in already known enzyme catalyzed reactions, but also in newly discovered or artificially engineered enzymes.…”

Section: Proposed Classification Of Structurally Different Fleeting C...mentioning

confidence: 99%

The Unexplored Importance of Fleeting Chiral Intermediates in Enzyme-Catalyzed Reactions

Reetz

Garcia‐Borràs

2021

J. Am. Chem. Soc.

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Decades of extensive research efforts by biochemists, organic chemists, and protein engineers have led to an understanding of the basic mechanisms of essentially all known types of enzymes, but in a formidable number of cases an essential aspect has been overlooked. The occurrence of short-lived chiral intermediates formed by symmetry-breaking of prochiral precursors in enzyme catalyzed reactions has been systematically neglected. We designate these elusive species as fleeting chiral intermediates and analyze such crucial questions as "Do such intermediates occur in homochiral form?" If so, what is the absolute configuration, and why did Nature choose that particular stereoisomeric form, even when the isolable final product may be achiral? Does the absolute configuration of a chiral product depend in any way on the absolute configuration of the fleeting chiral precursor? How does this affect the catalytic proficiency of the enzyme? If these issues continue to be unexplored, then an understanding of the mechanisms of many enzyme types remains incomplete. We have systematized the occurrence of these chiral intermediates according to their structures and enzyme types. This is followed by critical analyses of selected case studies and by final conclusions and perspectives. We hope that the fascinating concept of fleeting chiral intermediates will attract the attention of scientists, thereby opening an exciting new research field.

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Section: Proposed Classification Of Structurally Different Fleeting C...mentioning

confidence: 99%

The Unexplored Importance of Fleeting Chiral Intermediates in Enzyme-Catalyzed Reactions

Reetz

Garcia‐Borràs

2021

J. Am. Chem. Soc.

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“…Water is a polar dielectric and can undergo the phenomenon of electric polarization, reducing its configurational and vibrational entropy. Experiments with the transfer of quantum states showed that when the electric polarization of water changes, the catalytic activity of an intracellular enzyme, enolase, involved in the regulation of the glycolysis pathway, increases (Pietkiewicz et al, 2021). The permanent magnetic field induced by magnetite nanoparticles changes the mobility and orientation of hydrogen protons in resuspending solutions.…”

Section: Discussionmentioning

confidence: 99%

Glycolysis process activation in preserved red blood cells by nanotechnological treatment of resuspending solutions

Belousov

Malygon

Kalynychenko

et al. 2023

Regul. Mech. Biosyst.

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Currently, the use of nanotechnology opens up new opportunities to influence the processes of anaerobic glycolysis and the activity of hexose monophosphate reactions in preserved erythrocytes. Components containing donor red blood cells on CPDA-1 preservative were examined. Modified solutions of 0.9% NaCl and with 5% glucose were used as resuspending solutions. The solutions were treated with magnetite nanoparticles (ICNB brand) by the Belousov method. The amounts of 2,3-DPG, ATP, reduced glutathione, and glutathione peroxidase were determined by spectrophotometry. This study opens up new possibilities for increasing the shelf life and functional activity of preserved erythrocytes. The study showed a reliable increase in ATP and reduced glutathione, a decrease in 2,3-DPG and glutathione peroxidase. It was found that the activation of anaerobic glycolysis was less pronounced in tests with modified physiological saline than in tests with glucose solution. On the contrary, the pentose glucose oxidation cycle prevailed. A comprehensive analysis of the data obtained indicates the membrane-protective effect of the modified resuspending solutions. The membrane-protective effect is due to an increase in ATP and reduced glutathione, which ensures the redox potential of the cell in an equilibrium state. Magnetite nanoparticles (ICNB) change the mobility and orientation of hydrogen protons in resuspending solutions. This polarizes the aqueous sector of the erythrocyte microenvironment due to van der Waals forces, which is the main reason for activation of ATP phosphate residue hydrolysis and switching of intracellular enzymes regulating anaerobic glycolysis and pentose phosphate cycle into the active state. As a result, transmembrane metabolism and metabolism change, the energy state of erythrocytes changes, and enzymes are activated. All this has a significant impact on the energy supply of preserved red blood cells and preservation of their functional activity under storage conditions at 2 to 6 ºС.

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“…In the experiment, the quantum system used for an isothermal entropy reduction PER® is based on quantum bound entanglement of phonons [ 30 – 32 , and references therein] and on the phenomenon of phonon resonance (interference of phonon modes) in condensed matter (both silicon oxide, i.e. quartz (SiO 2 ) and single crystals of silicon (Si 0 ), aluminium (Alº), glass, and water).…”

Section: Methodsmentioning

confidence: 99%

“…quartz (SiO 2 ) and single crystals of silicon (Si 0 ), aluminium (Alº), glass, and water). The technology was first applied to reduce the entropy of the liquid medium and, consequently, to increase the efficiency of enzymatic processes (enolase enzyme) by about 30% 30 . The phonon signals in this experiment were transferred remotely over a distance of 160 km (from Gliwice to Wroclaw), by two sets of quantum-entangled PER® chips-inductors, each set transmitted phonon modes from one of two types of a generator which were single crystals of SiO 2 and Si 0 .…”

Section: Methodsmentioning

confidence: 99%

The isothermal Boltzmann–Gibbs entropy reduction affects survival of the fruit fly Drosophila melanogaster

Gruss,

Twardowski,

Samsel-Czekała

et al. 2023

Sci Rep

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To the best of our knowledge, this is the first experimental evidence of the effect of isothermal changes in entropy on a living organism. In greater detail, the effect of the reduction of the total Boltzmann–Gibbs entropy (S) of the aquatic environment on the survival rate and body mass of the fruit fly Drosophila melanogaster was investigated. The tests were carried out in standard thermodynamic states at room temperature of 296.15 K and ambient atmospheric pressure of 1 bar. Two variants of entropy reduction (ΔS) were tested for ΔS = 28.49 and 51.14 J K−1 mol−1 compared to the blind and control samples. The entropy level was experimentally changed, using the quantum system for isothermal entropy reduction. This system is based on quantum bound entanglement of phonons and the phenomenon of phonon resonance (interference of phonon modes) in condensed matter (Silicon dioxide (SiO2) and single crystals of Silicon (Si0), Aluminum (Al0) plates (“chips”), glass, and water). All studied organisms were of the same age (1 day). Mortality was observed daily until the natural death of the organisms. The investigations showed that changes in the Boltzmann–Gibbs entropy affected the survival and body mass of the fruit flies. On the one hand, the reduction in entropy under isothermal conditions in the aquatic environment for ΔS = 28.49 J K−1 mol−1 resulted in an extension of the lifespan and an increase in the body mass of female fruit flies. On the other hand, the almost twofold reduction in this entropy for ΔS = 51.14 J K−1 mol−1 shortened the lives of the males. Thus, the lifespan and body mass of flies turned out to be a specific reaction of metabolism related to changes in the entropy of the aquatic environment.

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Influence of Water Polarization Caused by Phonon Resonance on Catalytic Activity of Enolase

Cited by 3 publications

References 27 publications

The Unexplored Importance of Fleeting Chiral Intermediates in Enzyme-Catalyzed Reactions

The Unexplored Importance of Fleeting Chiral Intermediates in Enzyme-Catalyzed Reactions

Glycolysis process activation in preserved red blood cells by nanotechnological treatment of resuspending solutions

The isothermal Boltzmann–Gibbs entropy reduction affects survival of the fruit fly Drosophila melanogaster

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