Influence of white spot syndrome virus infection on hepatopancreas gene expression of ‘Huanghai No. 2’ shrimp (Fenneropenaeus chinensis)

Meng, Xianhong; Shi, Xiaoli; Kong, Jie; Luan, Sheng; Luo, Kun; Cao, Baoxiang; Liu, Ning; Lu, Xia; Li, Xupeng; Deng, Kailin; Cao, Jiawang; Zhang, Yingxue; Zhang, Hengheng

doi:10.1007/s11802-017-3250-7

Cited by 2 publications

(1 citation statement)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, contrary to its proposed involvement in shrimp immunity [97,98], as myosins are important factors related to growth performance [18], its hypothesised role in WSSV entry and trafficking may explain the high level of WSD susceptibility of P. vannamei that have been selectively bred for fast growth [99]. During late WSSV infection (48-96 hpi), a contrasting decrease in actins and myosins was reported in the hepatopancreas [100], which may also explain tissue-specific differences in susceptibility as the gills are a main target for WSSV infection. From 6 hpi, enhanced cytoskeletal processes were coupled with the upregulation of WSSV transcripts, such as WSSV thymidylate synthetase, that may offer the virus independence from host transcriptional machinery, and the enrichment of DNA-replication associated GO terms, such as deoxyribonucleotide biosynthetic process, are in line with prior literature stating viral replication begins at 6 hpi [101].…”

Section: Temporal Dynamics In Transcriptional Responses To Wssv Infection Illustrate Mechanisms Of Viral Entry and Replication During Earmentioning

confidence: 93%

Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp

Millard

Bickley

Bateman

et al. 2021

Viruses

View full text Add to dashboard Cite

White Spot Disease (WSD) presents a major barrier to penaeid shrimp production. Mechanisms underlying White Spot Syndrome Virus (WSSV) susceptibility in penaeids are poorly understood due to limited information related to early infection. We investigated mRNA and miRNA transcription in Penaeus vannamei over 36 h following infection. Over this time course, 6192 transcripts and 27 miRNAs were differentially expressed—with limited differential expression from 3–12 h post injection (hpi) and a more significant transcriptional response associated with the onset of disease symptoms (24 hpi). During early infection, regulated processes included cytoskeletal remodelling and alterations in phagocytic activity that may assist WSSV entry and translocation, novel miRNA-induced metabolic shifts, and the downregulation of ATP-dependent proton transporter subunits that may impair cellular recycling. During later infection, uncoupling of the electron transport chain may drive cellular dysfunction and lead to high mortalities in infected penaeids. We propose that post-transcriptional silencing of the immune priming gene Dscam (downregulated following infections) by a novel shrimp miRNA (Pva-pmiR-78; upregulated) as a potential mechanism preventing future recognition of WSSV that may be suppressed in surviving shrimp. Our findings improve our understanding of WSD pathogenesis in P. vannamei and provide potential avenues for future development of prophylactics and treatments.

show abstract

Section: Temporal Dynamics In Transcriptional Responses To Wssv Infection Illustrate Mechanisms Of Viral Entry and Replication During Earmentioning

confidence: 93%

Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp

Millard

Bickley

Bateman

et al. 2021

Viruses

View full text Add to dashboard Cite

show abstract

Association between ALFPm3 single nucleotide polymorphism and white spot syndrome virus resistance in black tiger shrimp Penaeus monodon

et al. 2020

View full text Add to dashboard Cite

Here single nucleotide polymorphisms (SNPs) were associated with white spot syndrome virus (WSSV) resistance in black tiger shrimp Penaeus monodon. SNPs were identified by single-strand conformation polymorphism (SSCP) screening and DNA sequencing of shrimp sampled from 3 families (100 shrimp per family) challenged with WSSV. Shrimp that died over the 14 d challenge trial were designated susceptible, with those remaining alive on Day 14 designated resistant. To compare SNPs, 10 samples from the susceptible and resistant groups, each comprising DNA pooled from 3 shrimp, were amplified by polymerase chain reaction (PCR) using primers to 12 selected genes and screened by SSCP. SNPs were only identified in the anti-lipopolysaccharide factor 3 (ALFPm3) gene product. Analysis of complete ALFPm3 gene sequences confirmed the existence of 3 SNPs (g.934C>G, g.1186A>G, and g.1898C>G) that were polymorphic between the susceptible and resistant groups. Further analyses using specific tetra-primer amplification refractory mutation system PCR primer sets associated these 3 SNPS, and particularly the g.1186A>G SNP, with WSSV resistance. This SNP thus has potential for use as a DNA marker to select for WSSV resistance in P. monodon breeding programs.

show abstract

Influence of white spot syndrome virus infection on hepatopancreas gene expression of ‘Huanghai No. 2’ shrimp (Fenneropenaeus chinensis)

Cited by 2 publications

References 64 publications

Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp

Global mRNA and miRNA Analysis Reveal Key Processes in the Initial Response to Infection with WSSV in the Pacific Whiteleg Shrimp

Association between ALFPm3 single nucleotide polymorphism and white spot syndrome virus resistance in black tiger shrimp Penaeus monodon

Contact Info

Product

Resources

About